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標題: | 紙式微流道結合時間閥進行紙式免疫分析 Automated Paper-Based Devices by Microfluidic Timing-Valve for Competitive ELISA |
作者: | Yu-Ting Lai 賴昱廷 |
指導教授: | 盧彥文(Yen-Wen Lu) |
關鍵字: | 免疫分析,微流道,益達安,時間閥,自動化, ELISA,paper-based,timing-valve,imidacloprid,automatic, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 台灣農藥檢測目前以儀器分析法為主,雖然儀器分析可以提供精準的結果,但儀器價錢昂貴、檢測過程繁雜、時間耗費較長、且需要專業的操作人員。與此相比,免疫分析法具有高專一性、快速、靈敏、及檢驗成本低等優點,其利用抗體抗原的專一性反應檢測樣品的含量,現今已被廣泛的應用在對於農藥的殘留分析上。然而,免疫分析通常需要一系列複雜的操作步驟,乏味且耗時。
為了將免疫分析的檢測方式普及化,例如應用於紙上,我們需要一種控制流體流動方法,來將複雜的操作步驟整合地且自動地執行,因此, 本研究利用時間閥(timing-valve)的方法配合紙式微流道的設計控制流體在紙上流動的順序,將進行免疫分析所需要的抗原抗體透過流體的操作依次傳輸到檢測區,時間閥的控制方式透過溶解不同濃度的介面活性劑來達到控制流體在紙上流動的順序。 在本論文中以益達安(Imidacloprid)為農藥殘留檢測的例子,並且成功的利用紙式微流道的設計配合時間閥的控制流體機制,達到在紙上進行免疫分析的操作,其結果證實了以簡單的紙式微流道裝置配合時間閥的設計,就可以在紙上進行多步驟的流體操作。 Pesticide residue detection has become increasingly important, as more and more evidence shows the relevance between pesticide exposure and other negative outcomes. Although analytical methods to detect pesticides have been demonstrated, they commonly suffer the need of high equipment cost, well-trained technicians, and complicated procedures. Meanwhile, one of the most common practices for pesticide residue detection is the enzyme-linked immunosorbent assay (ELISA). Recently, to apply ELISA onto a paper-based device as a convenient approach for biochemical analysis fields or pesticide residue detection has recently drawn great attention. Typical ELISA procedures often require a series of steps, including mixing, washing and incubation – these steps can be tedious and time consuming. Thus, a flow-controlling valve method, which allows these ELISA steps to be sequentially conducted, is the key for paper-based ELISA device. This thesis presents a timing-valve mechanism, which allows the complicated procedures of competitive ELISA procedures to be conducted on a paper-based device. The device consists of multiple channels, where the timing-valves regulate liquid flows to sequentially pass, mix and react for ELISA. The timing-valve is achieved using surfactants to dissolve the hydrophobic barriers and to allow fluid to pass through in a timed duration. Imidacloprid (e.g. small molecule pesticide) is tested in our proposed device and similar ELISA results were confirmed between traditional microplate and paper-based ELISA. A simplified fluidic manipulation procedure is thus validated, and it permits only single-step application of the sample solution for on-site detection. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78002 |
DOI: | 10.6342/NTU201700616 |
全文授權: | 有償授權 |
顯示於系所單位: | 生物機電工程學系 |
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