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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧彥文(Yen-Wen Lu) | |
dc.contributor.author | Yu-Ting Lai | en |
dc.contributor.author | 賴昱廷 | zh_TW |
dc.date.accessioned | 2021-07-11T14:39:22Z | - |
dc.date.available | 2022-09-29 | |
dc.date.copyright | 2017-09-29 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-02-16 | |
dc.identifier.citation | Abe, K., K. Suzuki and D. Citterio (2008). 'Inkjet-printed microfluidic multianalyte chemical sensing paper.' Analytical chemistry 80(18): 6928-6934.
Aharonson, N. (1987). 'Potential contamination of ground water by pesticides.' Pure and applied chemistry 59(10): 1419-1446. Baskaran, S., R. S. Kookana and R. Naidu (1997). 'Determination of the insecticide imidacloprid in water and soil using high-performance liquid chromatography.' Journal of Chromatography A 787(1): 271-275. Blasco, C., M. Fernández, Y. Picó, G. Font and J. Mañes (2002). 'Simultaneous determination of imidacloprid, carbendazim, methiocarb and hexythiazox in peaches and nectarines by liquid chromatography–mass spectrometry.' Analytica Chimica Acta 461(1): 109-116. Bonmatin, J., I. Moineau, R. Charvet, C. Fleche, M. Colin and E. Bengsch (2003). 'A LC/APCI-MS/MS method for analysis of imidacloprid in soils, in plants, and in pollens.' Analytical Chemistry 75(9): 2027-2033. Carrilho, E., A. W. Martinez and G. M. Whitesides (2009). 'Understanding wax printing: a simple micropatterning process for paper-based microfluidics.' Analytical chemistry 81(16): 7091-7095. Chen, H., J. Cogswell, C. Anagnostopoulos and M. Faghri (2012). 'A fluidic diode, valves, and a sequential-loading circuit fabricated on layered paper.' Lab on a Chip 12(16): 2909-2913. Cheng, C. M., A. W. Martinez, J. Gong, C. R. Mace, S. T. Phillips, E. Carrilho, K. A. Mirica and G. M. Whitesides (2010). 'Paper‐Based ELISA.' Angewandte Chemie International Edition 49(28): 4771-4774. Chin, C. D., T. Laksanasopin, Y. K. Cheung, D. Steinmiller, V. Linder, H. Parsa, J. Wang, H. Moore, R. Rouse and G. Umviligihozo (2011). 'Microfluidics-based diagnostics of infectious diseases in the developing world.' Nature medicine 17(8): 1015-1019. Dungchai, W., O. Chailapakul and C. S. Henry (2011). 'A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing.' Analyst 136(1): 77-82. Jeschke, P., R. Nauen, M. Schindler and A. Elbert (2011). 'Overview of the status and global strategy for neonicotinoids.' J Agric Food Chem. 59(7): 2897-2908. Abe, K., K. Suzuki and D. Citterio (2008). 'Inkjet-printed microfluidic multianalyte chemical sensing paper.' Analytical chemistry 80(18): 6928-6934. Aharonson, N. (1987). 'Potential contamination of ground water by pesticides.' Pure and applied chemistry 59(10): 1419-1446. Baskaran, S., R. S. Kookana and R. Naidu (1997). 'Determination of the insecticide imidacloprid in water and soil using high-performance liquid chromatography.' Journal of Chromatography A 787(1): 271-275. Blasco, C., M. Fernández, Y. Picó, G. Font and J. Mañes (2002). 'Simultaneous determination of imidacloprid, carbendazim, methiocarb and hexythiazox in peaches and nectarines by liquid chromatography–mass spectrometry.' Analytica Chimica Acta 461(1): 109-116. Bonmatin, J., I. Moineau, R. Charvet, C. Fleche, M. Colin and E. Bengsch (2003). 'A LC/APCI-MS/MS method for analysis of imidacloprid in soils, in plants, and in pollens.' Analytical Chemistry 75(9): 2027-2033. Carrilho, E., A. W. Martinez and G. M. Whitesides (2009). 'Understanding wax printing: a simple micropatterning process for paper-based microfluidics.' Analytical chemistry 81(16): 7091-7095. Chen, H., J. Cogswell, C. Anagnostopoulos and M. Faghri (2012). 'A fluidic diode, valves, and a sequential-loading circuit fabricated on layered paper.' Lab on a Chip 12(16): 2909-2913. Cheng, C. M., A. W. Martinez, J. Gong, C. R. Mace, S. T. Phillips, E. Carrilho, K. A. Mirica and G. M. Whitesides (2010). 'Paper‐Based ELISA.' Angewandte Chemie International Edition 49(28): 4771-4774. Chin, C. D., T. Laksanasopin, Y. K. Cheung, D. Steinmiller, V. Linder, H. Parsa, J. Wang, H. Moore, R. Rouse and G. Umviligihozo (2011). 'Microfluidics-based diagnostics of infectious diseases in the developing world.' Nature medicine 17(8): 1015-1019. Dungchai, W., O. Chailapakul and C. S. Henry (2011). 'A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing.' Analyst 136(1): 77-82. Eddleston, M. and D. N. Bateman (2012). 'Pesticides.' Medicine 40(3): 147-150. Hsu, C.-K., H.-Y. Huang, W.-R. Chen, W. Nishie, H. Ujiie, K. Natsuga, S.-T. Fan, H.-K. Wang, J. Y.-Y. Lee and W.-L. Tsai (2014). 'Paper-Based ELISA for the Detection of Autoimmune Antibodies in Body Fluid-The Case of Bullous Pemphigoid.' Analytical chemistry 86(9): 4605-4610. Jeschke, P., R. Nauen, M. Schindler and A. Elbert (2011). 'Overview of the status and global strategy for neonicotinoids.' J Agric Food Chem. 59(7): 2897-2908. Lei, K. F., C.-H. Huang, R.-L. Kuo, C.-K. Chang, K.-F. Chen, K.-C. Tsao and N.-M. Tsang (2015). 'Paper-based enzyme-free immunoassay for rapid detection and subtyping of influenza A H1N1 and H3N2 viruses.' Analytica chimica acta 883: 37-44. Lutz, B. R., P. Trinh, C. Ball, E. Fu and P. Yager (2011). 'Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.' Lab on a Chip 11(24): 4274-4278. Martinez, A. W., S. T. Phillips, M. J. Butte and G. M. Whitesides (2007). 'Patterned paper as a platform for inexpensive, low‐volume, portable bioassays.' Angewandte Chemie International Edition 46(8): 1318-1320. Martinez, A. W., S. T. Phillips, Z. Nie, C.-M. Cheng, E. Carrilho, B. J. Wiley and G. M. Whitesides (2010). 'Programmable diagnostic devices made from paper and tape.' Lab on a Chip 10(19): 2499-2504. Navalon, A., A. Gonzalez-Casado, R. El-Khattabi, J. L. Vilchez and A. R. Fernández-Alba (1997). 'Determination of imidacloprid in vegetable samples by gas chromatography–mass spectrometry.' Analyst 122(6): 579-581. Shih, C.-M., C.-L. Chang, M.-Y. Hsu, J.-Y. Lin, C.-M. Kuan, H.-K. Wang, C.-T. Huang, M.-C. Chung, K.-C. Huang and C.-E. Hsu (2015). 'Paper-based ELISA to rapidly detect Escherichia coli.' Talanta 145: 2-5. Songjaroen, T., W. Dungchai, O. Chailapakul and W. Laiwattanapaisal (2011). 'Novel, simple and low-cost alternative method for fabrication of paper-based microfluidics by wax dipping.' Talanta 85(5): 2587-2593. Van Emon, J. M., J. N. Seiber and B. D. Hammock (1989). 'Immunoassay techniques for pesticide analysis.' Analytical methods for pesticides and plant growth regulators 16: 217-263. Wang, T., M. Zhang, D. D. Dreher and Y. Zeng (2013). 'Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip.' Lab on a Chip 13(21): 4190-4197. Xing, C., L. Liu, S. Song, M. Feng, H. Kuang and C. Xu (2015). 'Ultrasensitive immunochromatographic assay for the simultaneous detection of five chemicals in drinking water.' Biosens Bioelectron. 66: 445-453 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78002 | - |
dc.description.abstract | 台灣農藥檢測目前以儀器分析法為主,雖然儀器分析可以提供精準的結果,但儀器價錢昂貴、檢測過程繁雜、時間耗費較長、且需要專業的操作人員。與此相比,免疫分析法具有高專一性、快速、靈敏、及檢驗成本低等優點,其利用抗體抗原的專一性反應檢測樣品的含量,現今已被廣泛的應用在對於農藥的殘留分析上。然而,免疫分析通常需要一系列複雜的操作步驟,乏味且耗時。
為了將免疫分析的檢測方式普及化,例如應用於紙上,我們需要一種控制流體流動方法,來將複雜的操作步驟整合地且自動地執行,因此, 本研究利用時間閥(timing-valve)的方法配合紙式微流道的設計控制流體在紙上流動的順序,將進行免疫分析所需要的抗原抗體透過流體的操作依次傳輸到檢測區,時間閥的控制方式透過溶解不同濃度的介面活性劑來達到控制流體在紙上流動的順序。 在本論文中以益達安(Imidacloprid)為農藥殘留檢測的例子,並且成功的利用紙式微流道的設計配合時間閥的控制流體機制,達到在紙上進行免疫分析的操作,其結果證實了以簡單的紙式微流道裝置配合時間閥的設計,就可以在紙上進行多步驟的流體操作。 | zh_TW |
dc.description.abstract | Pesticide residue detection has become increasingly important, as more and more evidence shows the relevance between pesticide exposure and other negative outcomes. Although analytical methods to detect pesticides have been demonstrated, they commonly suffer the need of high equipment cost, well-trained technicians, and complicated procedures.
Meanwhile, one of the most common practices for pesticide residue detection is the enzyme-linked immunosorbent assay (ELISA). Recently, to apply ELISA onto a paper-based device as a convenient approach for biochemical analysis fields or pesticide residue detection has recently drawn great attention. Typical ELISA procedures often require a series of steps, including mixing, washing and incubation – these steps can be tedious and time consuming. Thus, a flow-controlling valve method, which allows these ELISA steps to be sequentially conducted, is the key for paper-based ELISA device. This thesis presents a timing-valve mechanism, which allows the complicated procedures of competitive ELISA procedures to be conducted on a paper-based device. The device consists of multiple channels, where the timing-valves regulate liquid flows to sequentially pass, mix and react for ELISA. The timing-valve is achieved using surfactants to dissolve the hydrophobic barriers and to allow fluid to pass through in a timed duration. Imidacloprid (e.g. small molecule pesticide) is tested in our proposed device and similar ELISA results were confirmed between traditional microplate and paper-based ELISA. A simplified fluidic manipulation procedure is thus validated, and it permits only single-step application of the sample solution for on-site detection. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:39:22Z (GMT). No. of bitstreams: 1 ntu-106-R03631036-1.pdf: 2451665 bytes, checksum: 2229616c1f248c0271ea8cc92746caaa (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 中文摘要 i
Abstract ii Table of Contents iv List of Figures vii List of Tables xiii Chapter 1 Introduction 1 1.1 Pesticide and Pesticide Detection 1 1.2 Paper-Based Assay 3 1.3 Thesis Structure 3 Chapter 2 Literature Review 5 2.1 Fabrication Method 6 2.2 Paper-based ELISA 11 2.3 Valve and Switch 12 2.4 Lateral Flow Immunoassays (LFIA) 17 Chapter 3 Materials and Methods 20 3.1 ELISA and Indirect ELISA 20 3.1.1 Indirect Competitive ELISA Principle 20 3.1.2 Plate-based ELISA Procedure 22 3.2 Design and Fabrication of Our Paper-based Device 24 3.2.1 Timing-valve Principle 24 3.2.2 Device Design – First Generation 25 3.2.3 Device Design – Second Generation 26 3.2.4 Device Fabrication 31 3.3 Materials 32 3.4 Data Processing 33 3.5 Color Detection Program in the Android Platform 33 Chapter 4 Results and Discussions 35 4.1 The Enzyme-Linked Immunosorbent Assay (ELISA) 35 4.2 Optimization 36 4.2.1 Timing-valve 36 4.2.2 Membrane Blocking 42 4.2.3 Second Antibody (2nd Ab) 44 4.3 Performance of Automated Paper-based Devices 45 4.4 Android Application 49 4.5 Discussion 52 Chapter 5 Conclusions 55 5.1 Conclusions 55 5.2 Prospective 56 Appendix 1 58 References 61 | |
dc.language.iso | en | |
dc.title | 紙式微流道結合時間閥進行紙式免疫分析 | zh_TW |
dc.title | Automated Paper-Based Devices
by Microfluidic Timing-Valve for Competitive ELISA | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 周呈霙(Cheng-Ying Chou),許如君(Ju-Chun Hsu) | |
dc.subject.keyword | 免疫分析,微流道,益達安,時間閥,自動化, | zh_TW |
dc.subject.keyword | ELISA,paper-based,timing-valve,imidacloprid,automatic, | en |
dc.relation.page | 64 | |
dc.identifier.doi | 10.6342/NTU201700616 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-02-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
顯示於系所單位: | 生物機電工程學系 |
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