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標題: | 異源表現綠竹BoMSP41對於水稻生長及穀粒外觀及品質之影響 Effects of ectopic expression of Bambusa oldhamii MSP41 (BoMSP41) on the growth, grain appearance and quality of rice (Oryza sativa, L.) |
作者: | Wei-Han Shiu 徐暐涵 |
指導教授: | 王愛玉 |
共同指導教授: | 洪傳揚 |
關鍵字: | 種子大小,毛狀體,節間延長,白堊質,株高, seed size,trichome,internode elongation,chalkiness,plant height, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | BoMSP41 (Monocot specific protein-41 in Bambusa oldhamii) 為綠竹中之未知功能蛋白質。其基因表現量於綠竹較快速生長期的節間中大幅提升,可能參與綠竹生長。為了探討BoMSP41 的功能,先前已建立帶有FLAG-BoMSP41 或 BoMSP41- FLAG 之轉殖水稻(Oryza sativa L. cv Tainung 67)。本研究針對異源表現BoMSP41 對於轉殖株生長之影響進行觀察。比較野生株與轉殖株種子大小,發現轉殖株的種子長度增加,厚度縮小。另外也發現到,轉殖株內外穎上的毛狀體(trichome) 和野生株相比較為稀疏。此外,轉殖株的種子與野生株相比含有較高比例的白堊質。另外,轉殖株的穗長、第二及第三節的節間長度較野生株短,造成植株矮化的性狀。推測於水稻中異源表現BoMSP41 可能參與水稻節間中的細胞增生或細胞延長,毛狀體的分化及種子發育。 BoMSP41 (Monocot specific protein-41 in Bambusa oldhamii) is a protein with unknown function in bamboo. Its gene expression is highly up-regulated in the internode of bamboo during rapid growth stage, suggesting that BoMSP41 may involve in bamboo growth. To get insights into the function of BoMSP41, the FLAG-BoMSP41 or BoMSP41-FLAG gene have been introduced into rice (Oryza sativa L. cv Tainung 67) previously. The effect of ectopic expression of BoMSP41 on rice growth was investigated in this study. Comparing to the wild type, the seeds of transgenic rice showed an increase in seed length but a decrease in seed thickness. Besides, the density of trichomes on the lemma/palea of seed in transgenic plants was less than that of wildtype. Additionally, the seeds of transgenic rice contained higher content of chalkiness than wildtype. Moreover, the length of panicle, the second and the third internodes was decreased in transgenic plants, which resulted in a dwarf phenotype. The results suggest that ectopically expressed BoMSP41 may involve in cell proliferation and/or elongation in internodes, differentiation of trichome and seed development. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77964 |
DOI: | 10.6342/NTU201701721 |
全文授權: | 有償授權 |
顯示於系所單位: | 生化科技學系 |
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