利用微生物細胞聚集現象移除水中疏水性汙染物

Abstract

本實驗的研究目的主要在於探討利用細胞結塊的方式快速、大量地移除水中疏水性汙染物。 本實驗分別使用Rhodococcus erythropolis NTU-1以及未知的微生物NTU-X進行疏水性汙染物移除實驗，所用的兩種疏水性汙染物則為可溶於其他疏水性有機化合物的Sudan I(蘇丹紅)和不易溶於其他疏水性有機化合物的聚乙烯塑膠微粒。 在Sudan I移除實驗中發現，NTU-1和NTU-X皆能在短時間內移除大量的Sudan I：在700 ppm Sudan I的條件下，3天內，90%以上的Sudan I能被移除。移除Sudan I主要是藉由細胞聚集包覆Sudan I再將含有高濃度Sudan I的細胞結塊以物理法撈除，而非利用生物降解分解Sudan I，故能在短時間內移除大量汙染物。 在塑膠微粒移除實驗中，NTU-1和NTU-X也能在短時間內移除大量的塑膠微粒，且對不同粒徑的塑膠微粒也都具有良好的移除效果：700 ppm PE塑膠微粒的條件下，3天內，80%以上的PE塑膠微粒可被移除。其移除原理也是利用細胞聚集包覆PE塑膠微粒再將含有高濃度PE塑膠微粒的細胞結塊以物理法撈除。 利用NTU-1和NTU-X在正十六烷的環境下生長會產生細胞結塊的特性來移除水中疏水性汙染物，結合了物理移除及生物移除法之優點，能在短時間內移除高濃度之汙染物，此法為移除水中疏水性汙染物提供一個相當具競爭及發展性之移除方法。

This thesis focused on utilizing biofloccule formation of R. erythropolis NTU-1 and NTU-X to removal of hydrophobic pollutants in aqueous systems. In this study, two different kinds of hydrophobic pollutants were chosen. Sudan I, a kind of diazo dye, was used to represent oil-soluble hydrophobic pollutants in aqueous solutions. On the other hand, PE microplastics were selected to represent oil-insoluble hydrophobic pollutants in aqueous solutions. Results showed that both NTU-1 and NTU-X could rapidly remove high concentrations of containments. At least 90% Sudan I and 80% PE microplastics at the concentration of 700 ppm could be removed in 3 days. The main mechanism of removal of hydrophobic compounds is the aggregation of containments and cells, and followed subsequently physical separation. The utilizing of NTU-1 and NTU-X provided a perspective technique that had advantages of both physical and biological methods for remediation of removing hydrophobic compounds in aqueous systems.