以核黃素交聯膠原蛋白增強軟顎組織的機械性質

Abstract

打鼾是由於軟顎的塌陷導致上呼吸道阻塞，而氣流強行通過氣道所引起軟顎的震動並發出聲音就是我們常見的打鼾。本篇研究中，利用核黃素與UVA和膠原蛋白的交聯來增加組織的剛性，而用塗抹、注射和可溶性微針的方式來給予組織核黃素，並比較幾個常見的藥物傳遞方式的效果和效率。可溶性微針結合水溶性藥物是近幾年很有發展的給藥方式，並在實驗中測試其刺入組織的力和深度等特性，來評估所使用的製作微針的高分子濃度，最後選擇50% PVP來製作微針。根據我們的實驗結果，50% PVP微針包裹2% 核黃素相較於其他藥物傳遞方式有最好的效率使組織的剛性增加，而且在流變分析中，也證明經過治療的組織有較好的阻尼能力，並防止組織震動。綜上所述，本篇研究描述了特殊的可溶性微針結合水溶性藥物的方法，並證實可以穿透表皮組織，將藥物傳到較深的位置，使組織的剛性和阻尼能力增加。因此，本研究提供一個有潛力的治療方法和給藥工具來治療打鼾，並增加治療效果和病患的接受度與順應性。

Snoring is due to the collapse of the soft palates resulting in obstruction of the upper airway. In addition, the vibration of the soft palates caused by the air passing through the airway and making a sound are our common snoring. In this study, the crosslinking of riboflavin with UVA and collagen was used to increase the stiffness of the tissue. In this research, smearing, injection, and dissolving microneedles were used to administer riboflavin, and the effects and efficiencies of these drug delivery methods were compared. Dissolving microneedles combined with water-soluble drugs is a very developmental method of administration in recent years. In the experiments, the characteristics of the force and depth of the microneedles insertion into the tissue were tested to evaluate the polymer concentration of microneedles to used. Finally, 50% PVP was selected to fabricated microneedles. According to our results, 50% PVP microneedles contained 2% riboflavin have the best efficiency compared to other drug delivery methods to increase the stiffness of the tissue. And in the rheological analysis, it also proved that the treated tissue has better damping ability and prevents tissue vibration. In summary, this study describes a method of combining special soluble microneedles with water-soluble drugs. It was confirmed that it can penetrate the epidermal tissue and transfer the drug to a deeper position, which increases the stiffness and damping capacity of the tissue. Therefore, this study provides a potential therapeutic method and drug delivery tool to treat snoring, and increase the therapeutic effect and the acceptance and compliance of patients.