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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76725
標題: | 以X光為光源照射鈰參雜(銪參雜)碳酸鈣產生自由基用於腫瘤治療 Ce-doped (or Eu-doped) CaCO3 exposed to X-ray to induce free radicals for tumor treatment |
作者: | Wei-Yun Wang 王維筠 |
指導教授: | 林?輝 |
關鍵字: | 光動力治療,鈰參雜碳酸鈣,銪參雜碳酸鈣,X光,自由基, photodynamic therapy,cerium-doped calcium carbonate,europium-doped calcium carbonate,X-ray,free radical, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | Malignant neoplasms have been the first leading cause of death for consecutive 33 years in Taiwan. There are many kinds of therapeutic treatment for cancer. However, no matter what kind of therapy, side effect will always be there for patients. Photodynamic therapy limits the damage by utilizing its wavelength, it is used as a treatment targeting only surface cancer nowadays. In this study, cerium-doped calcium carbonate and europium-doped calcium carbonate are irradiated with higher penetration X-ray. With free radical generated, it can kill cancer cells and it is so called, 'photodynamic therapy.' We hope to apply the technique to deeper cancer treatments.
We successfully synthesize spherical porous cerium-doped calcium carbonate and europium-doped calcium carbonate by calcium nitrate and sodium carbonate. To enhance surface areas and accelerate reaction rate, polyvinyl alcohol and Tween 80 was added. We measure materials basic properties by using X-ray diffraction, scanning electron microscope and energy dispersive spectrometer. The X-ray diffraction pattern exhibits the characteristic reflection of vaterite. Cerium-doped calcium carbonate particle size is about 1-3 μm, and europium-doped calcium carbonate is about 4 μm. The energy dispersive spectrometer analysis shows that they have cerium and europium doping. Free radical generation detection was done by methylene blue test. Through ISO 10993 WST-1 and LDH test, both cerium-doped calcium carbonate and europium-doped calcium carbonate are shown to be biocompatible materials. LDH test and Live/Dead assay in vitro study show photodynamic therapy group killing more tumor cells than the group which only has the materials added without X-ray exposure. In vivo study, we have similar results. The tumor with cerium-doped calcium carbonate or europium-doped calcium carbonate injected to the tumor site and irradiated with X-ray groups have smaller tumor size than the group irradiated with only X-ray or untreated control group. At the twelfth day of this experiment, the cerium-doped calcium carbonate with X-ray group tumor size shrinks to 14.8% and europium-doped calcium carbonate with X-ray group tumor size shrinks to 5.4%. We can try to find out the perfect material concentration and X-ray dose to have a better tumor treatment effect in the future. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76725 |
DOI: | 10.6342/NTU201601528 |
全文授權: | 未授權 |
顯示於系所單位: | 材料科學與工程學系 |
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