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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林?輝 | |
| dc.contributor.author | Wei-Yun Wang | en |
| dc.contributor.author | 王維筠 | zh_TW |
| dc.date.accessioned | 2021-07-10T21:35:44Z | - |
| dc.date.available | 2021-07-10T21:35:44Z | - |
| dc.date.copyright | 2016-10-14 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-07-29 | |
| dc.identifier.citation | [1] 2014 statistics of causes of death. Ministry of Health and Welfare; 2015.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76725 | - |
| dc.description.abstract | Malignant neoplasms have been the first leading cause of death for consecutive 33 years in Taiwan. There are many kinds of therapeutic treatment for cancer. However, no matter what kind of therapy, side effect will always be there for patients. Photodynamic therapy limits the damage by utilizing its wavelength, it is used as a treatment targeting only surface cancer nowadays. In this study, cerium-doped calcium carbonate and europium-doped calcium carbonate are irradiated with higher penetration X-ray. With free radical generated, it can kill cancer cells and it is so called, 'photodynamic therapy.' We hope to apply the technique to deeper cancer treatments.
We successfully synthesize spherical porous cerium-doped calcium carbonate and europium-doped calcium carbonate by calcium nitrate and sodium carbonate. To enhance surface areas and accelerate reaction rate, polyvinyl alcohol and Tween 80 was added. We measure materials basic properties by using X-ray diffraction, scanning electron microscope and energy dispersive spectrometer. The X-ray diffraction pattern exhibits the characteristic reflection of vaterite. Cerium-doped calcium carbonate particle size is about 1-3 μm, and europium-doped calcium carbonate is about 4 μm. The energy dispersive spectrometer analysis shows that they have cerium and europium doping. Free radical generation detection was done by methylene blue test. Through ISO 10993 WST-1 and LDH test, both cerium-doped calcium carbonate and europium-doped calcium carbonate are shown to be biocompatible materials. LDH test and Live/Dead assay in vitro study show photodynamic therapy group killing more tumor cells than the group which only has the materials added without X-ray exposure. In vivo study, we have similar results. The tumor with cerium-doped calcium carbonate or europium-doped calcium carbonate injected to the tumor site and irradiated with X-ray groups have smaller tumor size than the group irradiated with only X-ray or untreated control group. At the twelfth day of this experiment, the cerium-doped calcium carbonate with X-ray group tumor size shrinks to 14.8% and europium-doped calcium carbonate with X-ray group tumor size shrinks to 5.4%. We can try to find out the perfect material concentration and X-ray dose to have a better tumor treatment effect in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:35:44Z (GMT). No. of bitstreams: 1 ntu-105-R03527011-1.pdf: 5178724 bytes, checksum: 14fc9800b739d7202916a718be39d5e4 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 論文口試委員會審定書 i
致謝 ii 中文摘要 iii ABSTRACT iv 目錄 vi 圖目錄 viii 表目錄 x 縮寫目錄 x 第一章 簡介 1 1.1 前言 1 1.2 治療方法 2 1.2.1 外科手術(Surgery) 2 1.2.2 化學藥物治療(Chemotherapy) 3 1.2.3 放射線治療(Radiation therapy) 3 1.2.4 免疫治療(Immunotherapy) 4 1.2.5 熱治療(Hyperthermia) 5 1.2.6 光動力療法(Photodynamic therapy) 5 1.3 光敏感藥物(Photosensitizer) 6 1.4 研究目的 8 第二章 理論基礎 9 2.1 光動力療法機制 9 2.2 主體材料-碳酸鈣 10 2.3 鈰及銪的參雜 10 2.4 細胞吞噬作用 11 第三章 實驗方法 12 3.1 實驗儀器 12 3.2 實驗藥品 13 3.3 實驗架構 15 3.4 材料製備 16 3.5 材料分析 17 3.5.1 X光繞射分析儀(XRD, X-ray Diffraction) 17 3.5.2 掃描式電子顯微鏡(SEM, Scanning Electron Microscope) 17 3.5.3 能量散射光譜儀(EDS, Energy Dispersive Spectrometer) 18 3.5.4 亞甲基藍測試 18 3.6 生物相容性測試 19 3.6.1 細胞株培養 19 3.6.2 材料萃取液製備 20 3.6.3 WST-1細胞活性測試 20 3.6.4 LDH細胞毒性測試 21 3.7 in vitro體外實驗 23 3.7.1 細胞株培養 23 3.7.2 照射光源 23 3.7.3 WST-1、LDH材料測試方法 23 3.7.4 LIVE/DEAD分析 24 3.8 in vivo體內實驗 25 3.8.1 實驗方法 25 3.8.2 安全性測試 26 3.9 統計分析 26 第四章 結果與討論 27 4.1 材料性質分析 27 4.1.1 X光繞射分析 27 4.1.2 表面型態分析 29 4.1.3 成分分析 31 4.1.4 亞甲基藍測試 33 4.2 生物相容性 34 4.2.1 WST-1細胞活性 34 4.2.2 LDH細胞毒性 35 4.3 in vitro體外實驗 36 4.3.1 WST-1細胞活性 36 4.3.2 LDH細胞毒性 38 4.3.3 LIVE/DEAD分析 40 4.4 in vivo體內實驗 44 4.4.1 存活率及體重變化 44 4.4.2 腫瘤大小變化 45 4.4.3 組織切片分析 48 4.4.4 血液分析 51 第五章 總結 53 參考文獻 54 | |
| dc.language.iso | zh-TW | |
| dc.title | 以X光為光源照射鈰參雜(銪參雜)碳酸鈣產生自由基用於腫瘤治療 | zh_TW |
| dc.title | Ce-doped (or Eu-doped) CaCO3 exposed to X-ray to induce free radicals for tumor treatment | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳克紹,楊禎明,張國基 | |
| dc.subject.keyword | 光動力治療,鈰參雜碳酸鈣,銪參雜碳酸鈣,X光,自由基, | zh_TW |
| dc.subject.keyword | photodynamic therapy,cerium-doped calcium carbonate,europium-doped calcium carbonate,X-ray,free radical, | en |
| dc.relation.page | 56 | |
| dc.identifier.doi | 10.6342/NTU201601528 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2016-07-30 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 材料科學與工程學系 | |
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