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Quantification and Analysis of Chicken Cornea during Development
|Publication Year :||2016|
The optical microscope has been developed for many centuries of science, first with the invention and use of lenses, and then with the perfection of lenses and illumination. Today, due to the integrity, fineness and nondestructive of measurement, optical microscopy has been widely used in the biological and medical imaging.
Cornea, the outermost layer of the eye, is mainly composed of fibrotic collagen. Any distortion of cornea collagen orientation can result in significant visual deterioration. In order to understand how corneal collagen is organized and the probability of fabricating the artificial cornea to cure corneal diseases. It is an important work to realize the development of cornea.
In this thesis, the main purpose is to obtain the development of chicken embryo cornea. Leghorn species was chosen as the experiment animal, for observing the collagen pattern and measuring the size, also the transparency of cornea. We first utilized high resolution optical microscopy to show the detail of structure. Then we used the dissecting microscope and Vernier caliper to evaluate the dimensions of cornea during growth. At last, the spectrometer was used to study the transparency in the wavelength range of 200 nm -1000 nm. The observation period of chicken embryo cornea development started from 10 to 19 day of post fertilization. Three embryos were used to support the reliability of the experiment for each day of post fertilization. The result of this study not only shows the growth details of cornea, but also provides the valuable information for us to inference the internal structure of chicken embryo.
|Appears in Collections:||物理學系|
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