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標題: | 以氫氧基磷灰石奈米粒子載體摻雜鈰做為癌症療法之研究 The Study of HAp Doped with Cerium as a Active Particle for Cancer Therapy |
作者: | Yu-Hsiang Ni 倪鈺翔 |
指導教授: | 林?輝 |
關鍵字: | 氫氧基磷灰石,鈰離子,光動力治療, hydroxyapatite,cerium,photodynamic therapy, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 癌症在現今社會癌症是重要的健康問題,目前對於癌症的治療方法皆有優缺點,而其中光動力治療是非侵入性、對人體的副作用小、能進行標靶性治療的優點,但是其受到光線本身穿透性的限制導致治療的深度有限。近年來許多針對金屬氧化物和錯合物的奈米粒子進行照射高能輻射線的腫瘤生長研究,發現照射後能產生大量的活性氧化物 (reactive oxygen species; ROS) ,並且奈米粒子不會屯聚在周圍的正常組織,並能治療深層的腫瘤,但這些奈米粒子並非可被生物降解 (biodegradable) 。
考量到磷酸鈣陶瓷系統 (calcium phosphate) 與人體骨骼和牙齒擁有類似的組成成分,且擁有優異的生物相容性以及生物活性,所以近年來普遍被應用於生物醫學領域,而其中又以氫氧基磷灰石 (hydroxyapatite; HAp) 的應用最為常見,主要的原因是氫氧基磷灰石擁有高度的穩定性,不容易受pH值、溫度及生物環境影響而變質,因此將金屬元素摻雜進氫氧基磷灰石的奈米粒子再施以X光照射,以期能達到與氧化物和錯合物的奈米粒子類似的效果。 本研究使用氫氧基磷灰石奈米粒子作為載體,將鈰摻雜進去,再施以X光照射,在WST-1測試結果顯示摻雜鈰之氫氧基磷灰石擁有良好的生物相容性,在照X光的LDH測試顯示此材料具有毒殺肺癌細胞A549的效果。 Cancer is an important healthy issue in the society. The treatments of cancer have pros and cons respectively, and photodynamic therapy (PDT) is noninvasive, targeted therapy, has minimal side effect among them. However, the treatment depth of PDT is limited by the penetration of light. Many researches study the metal oxides and complexes nanoparticles on the apoptosis of tumors after radiation, and then they found after irradiation these nanoparticles produce numerous reactive oxygen species (ROS), furthermore, these particles don’t accumulate at normal tissue around the tumor, cure tumor that is at deeper site. Nerveless, these nanoparticles are not biodegradable. Consider the calcium phosphate ceramic system is the composition of human bones and teeth, and have excellent biocompatibility and bioavailability, so calcium phosphate system is used in biomedical application widely. Due to the high stability at the change of pH value, temperature and biological environment, Hydroxyapatite (HAp) is the most common. We doped metal element into HAp nanoparticles and observe the apoptosis of cancer cells after giving a certain x-ray irradiation dose. We expect the effect is the same to metal oxides nanoparticles. In this study, the HAp nanoparticles are used as bio-carrier that are doped with cerium. The WST-1 assay shows good biocompatibility of Ce-doped HAp and the LDH assay with x-ray irradiation shows that the Ce-doped HAp can kill A549 cells after radiation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76689 |
DOI: | 10.6342/NTU201602550 |
全文授權: | 未授權 |
顯示於系所單位: | 材料科學與工程學系 |
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