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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76689完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林?輝 | |
| dc.contributor.author | Yu-Hsiang Ni | en |
| dc.contributor.author | 倪鈺翔 | zh_TW |
| dc.date.accessioned | 2021-07-10T21:35:07Z | - |
| dc.date.available | 2021-07-10T21:35:07Z | - |
| dc.date.copyright | 2016-10-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-16 | |
| dc.identifier.citation | 1. 行政院衛生福利部網站, 2014.
2. World Health Organization web site. 3. Niederhuber JE et al, Abeloff's Clinical Oncology, 4th edition, 407-416, 2008. 4. Olivier Cyrille Louis Haas, Radiotherapy Treatment Planning: New System Approaches, 1-32, 1999. 5. Deborah Tomlinson Nancy E. Kline, Pediatric Oncology Nursing, chapter8, 161-193, 2005. 6. Abdul Saied et al, Immunotherapy for solid tumors - a review for surgeons. Journal of Surgical Research, 525-535, 1999. 7. P.R. Stauffer, Hyperthermia Therapy for Cancer, Physical Medicine and Rehabilitation, 115–151, 2014. 8. Byoung-chan Bae, Development of Polymeric Cargo for Delivery of Photosensitizer in Photodynamic Therapy, International Journal of Photoenergy, 14 pages, 2012. 9. Lei Teng et al, Current Applications of 5–ALA in Glioma Diagnostics and Therapy, Clinical Management and Evolving Novel Therapeutic Strategies for Patients with Brain Tumors, chapter 12, 2013. 10. Caroline M Moore et al, Photodynamic therapy for prostate cancer—a review of current status and future promise. Nature Reviews Urology 6, 18-30, 2009. 11. Ti-Chen Chen et al, Luminol as the light source for in situ photodynamic therapy, Process Biochemistry, 1903–1908, 2012. 12. Laurence Maggiorella et al, Nanoscale radiotherapy with hafnium oxide nanoparticles. Future Oncol 8(9), 1167–1181, 2012. 13. Deep Kwatra et al. Nanoparticles in radiation therapy: a summary of various approaches to enhance radiosensitization in cancer, Transl Cancer Res 2(4), 330-342, 2013. 14. Vuk Uskokovic´ et al, Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents, Journal of Biomedical Materials Research B: Applied Biomaterials, 152-191, 2011. 15. M. A. Jakupec et al, Pharmacological properties of cerium compounds, Physiology Biochemistry and Pharmacology, 101-111, 2005. 16. Ramovatar Meena et al, Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7), Journal of Nanoparticle Research 14:712, 2012. 17. Zhongli Shi et al, Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cell, Acta Biomaterialia, 338–345, 2009. 18. Sheng-Hua Chu et al, in vitro and in vivo radiosensitization induced by hydroxyapatite nanoparticles, Neuro-Oncology 15(7), 880-890, 2013. 19. Helen E. Townley et al, Nanoparticle augmented radiation treatment decreases cancercell proliferation, Nanomedicine: Nanotechnology, Biology, and Medicine 8,526–536, 2012. 20. Stefanie Klein et al, Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells, Biochemical and Biophysical Research Communications, 217–222, 2013. 21. Pedro M. David Gara et al, ROS enhancement by silicon nanoparticles in X-ray irradiated aqueous suspensions and in glioma C6 cells. Journal of Nanoparticle Research 14:741, 2012. 22. Alexander Peetsch et al, Silver-doped calcium phosphate nanoparticles: Synthesis, characterization, and toxic effects toward mammalian and prokaryotic cells, Colloids and Surfaces B: Biointerfaces, 724-729, 2013. 23. C.S. CIOBANU et al, BET and XRD studies on the hydroxyapatite and europium doped hydroxyapatite, Journal of Optoelectronics and Advanced Materials, 821- 824, 2011. 24. Matthew Bilton et al, Comparison of Hydrothermal and Sol-Gel Synthesis of Nano-Particulate Hydroxyapatite by Characterisation at the Bulk and Particle Level, Open Journal of Inorganic Non-metallic Materials, 1-10, 2012. 25. Ying Xue et al, The Vital Role of Buffer Anions in the Antioxidant Activity of CeO2 Nanoparticles, Chemistry - A European Journal, 11115–11122, 2012. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76689 | - |
| dc.description.abstract | 癌症在現今社會癌症是重要的健康問題，目前對於癌症的治療方法皆有優缺點，而其中光動力治療是非侵入性、對人體的副作用小、能進行標靶性治療的優點，但是其受到光線本身穿透性的限制導致治療的深度有限。近年來許多針對金屬氧化物和錯合物的奈米粒子進行照射高能輻射線的腫瘤生長研究，發現照射後能產生大量的活性氧化物 (reactive oxygen species; ROS) ，並且奈米粒子不會屯聚在周圍的正常組織，並能治療深層的腫瘤，但這些奈米粒子並非可被生物降解 (biodegradable) 。
考量到磷酸鈣陶瓷系統 (calcium phosphate) 與人體骨骼和牙齒擁有類似的組成成分，且擁有優異的生物相容性以及生物活性，所以近年來普遍被應用於生物醫學領域，而其中又以氫氧基磷灰石 (hydroxyapatite; HAp) 的應用最為常見，主要的原因是氫氧基磷灰石擁有高度的穩定性，不容易受pH值、溫度及生物環境影響而變質，因此將金屬元素摻雜進氫氧基磷灰石的奈米粒子再施以X光照射，以期能達到與氧化物和錯合物的奈米粒子類似的效果。 本研究使用氫氧基磷灰石奈米粒子作為載體，將鈰摻雜進去，再施以X光照射，在WST-1測試結果顯示摻雜鈰之氫氧基磷灰石擁有良好的生物相容性，在照X光的LDH測試顯示此材料具有毒殺肺癌細胞A549的效果。 | zh_TW |
| dc.description.abstract | Cancer is an important healthy issue in the society. The treatments of cancer have pros and cons respectively, and photodynamic therapy (PDT) is noninvasive, targeted therapy, has minimal side effect among them. However, the treatment depth of PDT is limited by the penetration of light. Many researches study the metal oxides and complexes nanoparticles on the apoptosis of tumors after radiation, and then they found after irradiation these nanoparticles produce numerous reactive oxygen species (ROS), furthermore, these particles don’t accumulate at normal tissue around the tumor, cure tumor that is at deeper site. Nerveless, these nanoparticles are not biodegradable.
Consider the calcium phosphate ceramic system is the composition of human bones and teeth, and have excellent biocompatibility and bioavailability, so calcium phosphate system is used in biomedical application widely. Due to the high stability at the change of pH value, temperature and biological environment, Hydroxyapatite (HAp) is the most common. We doped metal element into HAp nanoparticles and observe the apoptosis of cancer cells after giving a certain x-ray irradiation dose. We expect the effect is the same to metal oxides nanoparticles. In this study, the HAp nanoparticles are used as bio-carrier that are doped with cerium. The WST-1 assay shows good biocompatibility of Ce-doped HAp and the LDH assay with x-ray irradiation shows that the Ce-doped HAp can kill A549 cells after radiation. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:35:07Z (GMT). No. of bitstreams: 1 ntu-105-R02527022-1.pdf: 1381382 bytes, checksum: 98aa9c95fdd461343c37594a56a415df (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 中文摘要.........................................................................................................................i
Abstract..........................................................................................................................ii 目錄...............................................................................................................................iii 圖目錄............................................................................................................................v 表目錄..........................................................................................................................vii 第一章 簡介..................................................................................................................1 1-1 前言.....................................................................................................................1 1-2 癌症.....................................................................................................................2 1-3 癌症治療.............................................................................................................3 1-3-1 手術切除.....................................................................................................3 1-3-2 放射線療法.................................................................................................3 1-3-3 化學藥物療法.............................................................................................4 1-3-4 免疫療法.....................................................................................................5 1-3-5 熱治療.........................................................................................................6 1-3-6 光動力治療.................................................................................................6 1-4 光動力治療機制.................................................................................................7 1-5 體內的光動力治療.............................................................................................8 1-6 奈米粒子於癌症治療的應用.............................................................................9 1-7 研究目的...........................................................................................................10 1-8 奈米粒子對細胞的影響...................................................................................11 第二章 材料與方法....................................................................................................13 2-1 實驗儀器...........................................................................................................13 2-2 實驗藥品...........................................................................................................15 2-3 實驗流程與材料製備.......................................................................................16 2-3-1 實驗架構...................................................................................................16 2-3-2 摻雜鈰之氫氧基磷灰石奈米粒子製備...................................................17 2-4 材料分析以及生物相容性測試.......................................................................18 2-4-1 X光繞射分析儀.........................................................................................18 2-4-2 穿透式電子顯微鏡...................................................................................19 2-4-3 X光能譜分析儀.........................................................................................19 2-4-4 X射線光電子能譜儀.................................................................................20 2-4-5 感應耦合電漿質譜儀...............................................................................21 2-4-6 WST-1生物相容性測試............................................................................22 2-4-7 LDH細胞毒性測試....................................................................................24 2-4-8 照射X光之細胞毒性測試.......................................................................25 第三章 結果與討論....................................................................................................26 3-1 摻雜鈰之氫氧基磷灰石奈米粒子之材料性質分析.......................................26 3-1-1 X光繞射圖譜分析 (XRD)........................................................................26 3-1-2 穿透式電子顯微鏡分析 (TEM)..............................................................27 3-1-3 X光能譜分析儀 (EDS).............................................................................29 3-1-4 X射線光電子能譜儀分析 (XPS).............................................................31 3-1-5 感應耦合電漿質譜儀分析 (ICP-MS).....................................................33 3-2 摻雜鈰之氫氧基磷灰石奈米粒子之生物方面分析.......................................34 3-2-1 生物相容性測試.......................................................................................34 3-2-2 細胞毒性測試...........................................................................................35 3-2-3 進行照光的細胞毒性測試...................................................................... 36 第四章 結論................................................................................................................37 第五章 參考文獻........................................................................................................38 | |
| dc.language.iso | zh-TW | |
| dc.title | 以氫氧基磷灰石奈米粒子載體摻雜鈰做為癌症療法之研究 | zh_TW |
| dc.title | The Study of HAp Doped with Cerium as a Active Particle for Cancer Therapy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊禎明,張淑真,郭士民 | |
| dc.subject.keyword | 氫氧基磷灰石,鈰離子,光動力治療, | zh_TW |
| dc.subject.keyword | hydroxyapatite,cerium,photodynamic therapy, | en |
| dc.relation.page | 40 | |
| dc.identifier.doi | 10.6342/NTU201602550 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2016-08-17 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 材料科學與工程學系 | |
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