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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76380
標題: | 利用一種重複性DNA序列的分佈建立縐葉菸草的核型並定位5S和26S rRNA基因 Karyotype of Nicotiana plumbaginifolia based on distribution of a repetitive DNA sequence and mapping of 5S and 26S rRNA genes |
作者: | Chung-Ju Wang 王中茹 |
出版年 : | 1998 |
學位: | 碩士 |
摘要: | 縐葉菸草(Nicotiana plumbaginifolia)有十對末端中節或近端中節的染色體,利用傳統細胞學的技術無法輕易地將每一對染色體加以區別。利用螢光原位雜交(fluorescence in situ hybridization, FISH)的技術,藉著一個稱為NP3R的重複性DNA序列在染色體上的分佈,建立N. plumbaginifolia的核型。NP3R是一個165 bp頭尾相接的重複性序列(tandemly repetitive sequence),在十對染色體上的分佈由於有位置和數量的差異,故能清楚地鑑別N. plumbaginifolia的每一對染色體。此外,利用原位雜交重複標定和多顏色螢光原位雜交(multi-color FISH)的方法,將5S rRNA基因定位於第二對染色體長臂的近端,26S rRNA基因定位在第九對及第十對染色體的末端。 Nicotiana plumbaginifolia has 10 pairs of acrocentric or telocentric chromosomes, which could not be distinguished from each other by conventional cytological techniques. The somatic karyotype of this species was constructed based on fluorescence in situ hybridization (FISH) to a 165 bp tandemly repeated sequence, NP3R. Variation in the spatial and quantitative distribution of this repeat allowed the identification of each of the 10 pairs of N. plumbaginifolia chromosomes. Two-step FISH and multi-color FISH showed that 5S rDNA was clustered in a proximal region in the long arm of chromosome 2, and that 26S rDNA was clustered in the terminal regions of chromosomes 9 and 10. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76380 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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