一 PHD finger 蛋白質的 cDNA 選殖及其在自體免疫病人自體抗體之偵測

Abstract

本論文研究一段新的基因，它所encode的protein具有PHD/ LAP finger，命名為Requiem 6，文中除了研究細胞中它的messenger RNA及蛋白質表現情形之外，也找到了全長cDNA，同時發現Requiem 6與DNA-dependent protein kinase catalytic subunit (DNA-PKcs)存在於同一個complex中。 同樣會與 DNA-dependent protein kinase (DNA-PKcs)形成complex的Ku protein早在1985年就知道它是自體抗原（autoantigen）。而DNA-PKcs是1996年才被發現為自體抗原，同時發現DNA-PKcs自體抗體與Ku protein自體抗體的存在，兩者之間有正相關性。本論文在氣喘(asthma)、全身性硬化症、疑似palindromic syndrome、紅斑性狼瘡(SLE)、乾性角膜結合膜炎（Sj?gren's syndrome）、類風濕關節炎(rheumatoid arthritis)這類自體免疫病患中測得Requiem 6自體抗體，而有Requiem 6自體抗體的病人會伴隨著DNA-PKcs自體抗體存在，卻不一定有Ku protein自體抗體。

cDNA encoding a PHD Finger protein, Requiem 6, was cloned. The expression of Requiem 6 mRNA was found in many tissues and cells .Requiem 6 is associated with a complex containing DNA-dependent protein kinase catalytic subunit (DNA-PKcs). Recently, patients have been identified with antibodies to the DNA-PKcs that appears to be associated with anti-Ku antibody. Anti-Requiem 6 auto-antibody was found to be present in patients of systemic lupus erythematosus, Sj?gren's syndrome, rheumatoid arthritis, asthma, palindromic syndrome and multiple sclerosis. The appearance of anti-Requiem 6 and anti-DNA-PKcs auto-antibodies in patients with autoimmune conditions is strikingly similar. These results suggest that Requiem 6 and DNA-PKcs exist in the same complex.