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標題: | 小白鼠細胞素IL-10的訊息傳遞及對轉錄因數AGP/EBP的調控 Signal Transduction of Mouse Interleukin 10 and Regulation of AGP/EBP |
作者: | 林主國 |
出版年 : | 1994 |
學位: | 碩士 |
摘要: | 近年來在細胞素( cytokine )之訊息傳遞( signal transduction )方面的研究有相當的進展, STAT ( signal transduction and activation of transcription )家族被發現參與許多細胞素的訊息傳遞路徑, STAT 家族具有訊息傳遞以及轉錄活化之雙重功能,其中 ISGF-3α p91 (又名 STAT1α )參與幹擾素 α/β、 γ(IFNα/β、γ)之訊息傳遞路徑,並可調控早期基因( early gene )的表現,為了瞭解 p91 與轉錄因數 AGP/EBP 之間的關係,我製作了抗 P91 之單株抗體,利用抗體為工具,進一步研究 p91 與 AGP/EBP 之間的關係。 此外, mIL-10 為一新的細胞素,關於其訊息傳遞及標的法因的瞭解都不多,雖已知在單核細胞內與 p91 之活化有關,但本論文發現在小白鼠 C127 乳線瘤細胞中仍是由 p91 家族參與傳訊過程。 最後,本論文又試著探討 mIL對 AGP/EBP 基因之調控,發現mIL-10在 C127 細胞中可以促進 AGP 甩 BP 在 RNA 上之表現,所以mIL-10、 P91 與 AGP/EBP 三者之間可能存在著某些微妙的關係。 In recent years, considerable progress has been made on signal transduction pathways of different cytokines. The STAT family of proteins, carrying out dual functions: signal transduction and activation of transcription, are involved in many cytokine-induced signal transductions. p91 (ISGF-3α, STAT1α) was originally identified to be involved in IFN α/β, γ signalling pathway. It has been shown that the activated p91 can stimulate the expression of a set of early genes. In this thesis, anti-p91 monoclonal antibodies were prepared and subsequently used for investigation of IL-10 signal transduction. The signalling pathway of mIL-10 was studied. mIL-10 can activate p91 in monocytes. It can also induce the translocation of p91 family proteins from cytosol to nucleus in C127 cell. mIL-10 can induce the expression of AGPIEBP gene. However, the role of p91 in the mIL-10-induced expression of AGP/EBP remains to be clarified. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76021 |
全文授權: | 未授權 |
顯示於系所單位: | 生化科學研究所 |
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