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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林主國 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:18Z | - |
| dc.date.available | 2021-07-01T08:17:18Z | - |
| dc.date.issued | 1994 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76021 | - |
| dc.description.abstract | 近年來在細胞素（ cytokine ）之訊息傳遞（ signal transduction ）方面的研究有相當的進展， STAT ( signal transduction and activation of transcription ）家族被發現參與許多細胞素的訊息傳遞路徑， STAT 家族具有訊息傳遞以及轉錄活化之雙重功能，其中 ISGF－3α p91 （又名 STAT1α ）參與幹擾素 α/β、 γ（IFNα/β、γ）之訊息傳遞路徑，並可調控早期基因（ early gene ）的表現，為了瞭解 p91 與轉錄因數 AGP/EBP 之間的關係，我製作了抗 P91 之單株抗體，利用抗體為工具，進一步研究 p91 與 AGP/EBP 之間的關係。 此外， mIL-10 為一新的細胞素，關於其訊息傳遞及標的法因的瞭解都不多，雖已知在單核細胞內與 p91 之活化有關，但本論文發現在小白鼠 C127 乳線瘤細胞中仍是由 p91 家族參與傳訊過程。 最後，本論文又試著探討 mIL對 AGP/EBP 基因之調控，發現mIL-10在 C127 細胞中可以促進 AGP 甩 BP 在 RNA 上之表現，所以mIL-10、 P91 與 AGP/EBP 三者之間可能存在著某些微妙的關係。 | zh_TW |
| dc.description.abstract | In recent years, considerable progress has been made on signal transduction pathways of different cytokines. The STAT family of proteins, carrying out dual functions: signal transduction and activation of transcription, are involved in many cytokine-induced signal transductions. p91 (ISGF-3α, STAT1α) was originally identified to be involved in IFN α/β, γ signalling pathway. It has been shown that the activated p91 can stimulate the expression of a set of early genes. In this thesis, anti-p91 monoclonal antibodies were prepared and subsequently used for investigation of IL-10 signal transduction. The signalling pathway of mIL-10 was studied. mIL-10 can activate p91 in monocytes. It can also induce the translocation of p91 family proteins from cytosol to nucleus in C127 cell. mIL-10 can induce the expression of AGPIEBP gene. However, the role of p91 in the mIL-10-induced expression of AGP/EBP remains to be clarified. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:18Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 中文摘要（Chinese abstract）……………………………………1 英文摘要（English abstract）……………………………………2 第一章 概論（Introduction)……………………………………3 第二章 材料與方法（Materials & Methods）…………………8 一、 選殖人類ISGF-3α, p91片段基因至蛋白質表現載體……8 二、 以鎳離子管柱?Nickel (II) column?純化融合蛋白質……9 三、 抗人類ISGF-3α, p91單秣抗體之製作……………………11 四、 以Baculovirus生產細胞素mouse Interleukin-10………13 五、 老鼠乳線瘤C127細胞核萃取液及細胞質液之抽取……14 六、 免疫沈澱法（Immunoprecipitation）…………………16 七、 聚丙烯醯胺膠體電泳（SDS-PAGE）……………………18 八、 西方墨點法(Western Blot）……………………………21 九、 C127細胞核醣核酸（RNA）之抽取…………………23 十、 北方墨點法（Northern Blot）……………………………24 十一、 細胞轉染（Transfection）…………………………27 十二、 Chloramphenicol Acetyl Transferase(CAT)Assay…28 第三章 實驗結果（Results）…………………………………30 第四章 討論（Discussions)……………………………………52 參考文獻（References)…………………………………………56 | |
| dc.language.iso | zh-TW | |
| dc.title | 小白鼠細胞素IL－10的訊息傳遞及對轉錄因數AGP/EBP的調控 | zh_TW |
| dc.title | Signal Transduction of Mouse Interleukin 10 and Regulation of AGP/EBP | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 62 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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