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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | C.H.WONG | en |
| dc.contributor.author | 翁?惠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:52Z | - |
| dc.date.available | 2021-07-01T08:16:52Z | - |
| dc.date.issued | 1977 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75968 | - |
| dc.description.abstract | 台灣飯匙倩蛇毒心藏毒由60個氨基酸所構成，其中含四對雙硫鍵。為瞭解構造與生物活性的關係，首先以化學合成方法加以探討。 經由逐步固相合成及片斷固相合成所得產物以液態氟化氫除去所有保護基，然後以分子篩過濾分離出還原態心臟毒。再將其置於適當水溶液中令其自動氧化並進行立體特異組合而成氧化態心臟毒。粗產物經分子篩過瀘，陽離子交換，及親和柱層分離重複純化，精製而成人工合成心臟毒。最後收量以片斷固相合成法較高（0.5%)，且較容易純化。 人工合成心臟毒其氨基酸組成分析，電泳分析，薄層色層分析及 CD -光譜分析發現與天然心臟毒相同。免疫活性，頸肌收縮活性及毒性試驗也與天然所表現相同（約90%)。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1977 | en |
| dc.description.tableofcontents | I．Intruduction…………………………………………………………………………1 I-1 Structure and function of cardiotoxin………………………………………………1 I-2 Propose of the chemical synthesis………………………………………………………1 I-3 Stwategy and tactics of the synthesis…………………………………………………1 II. Experimental methods……………………………………………………………………9 II-1.Symbols of abbreviations and the materials used……………………………………9 II-2 Physical, chemical characterization of the synthetic product…………………10 II-3 Preparation of amino acid derivative……………………………………………12 II-4 Preparation of peptide fragment…………………………………………………12 II-4-1 DCC-method………………………………………………………………………12 II-4-2 DCC-HONSu method……………………………………………………………13 II-4-3 DCC-HOBt method……………………………………………………………13 II-4-4 Active ester method…..…………………………………………………………13 II-5 Attachment of C-terminal amino acid to resin…………………………………14 II-6 Stepwise solid phase synthesis of protected polypeptide……………………15 II-7 Fragment solid phase synthesis of protected peptide…………………………15 II-8 Liquid HF cleavage…………………………………………………………………16 II-9 Refolding of reduced natural CTX………………………………………………17 II-10 Purification of the synthetic CTX in a reduced state the folding……………17 II-10-1 For the fragment solid phase synthesis………………………………………17 II-10-2 For the stepwise solid phase synthesis………………………………………18 II-11 Purification of the synthesized CTX after oxidation………………………….18 II_12Preparation of anti-CTX sera and immunoadsorbant………………………..18 II-13 The bioassay……………………………19 III.RESULTS..........................................................39 III-1 Preparation of protected amino acid and peptide fragment for solid phase synthesis…………39 III-2 Refolding of reduced natural cardiotoxin………………………………39 III-3 The stepwise solid phase synthesis of cardiotoxin ………………40 III-4 The fragment solid phase synthesis of cardiotoxin …………………41 IV. Discussion………………………………62 V. Acknowledgement…………………63 VI References…………………………64 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣飯匙倩蛇毒心?毒之化學合成 | zh_TW |
| dc.title | TOTAL SYNTHESIS OF TAIWAN COBRA CARDIOTOXIN | en |
| dc.date.schoolyear | 65-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| Appears in Collections: | 生化科學研究所 | |
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