篩選具有硝化及脫氮能力的細菌

Abstract

本實驗以本省含氮廢棄物做?分離源，篩選兼具硝化及脫氮作用的細菌，並利用菌落雜交技術挑出能與國外購買之Thiosphaera pantotrpha之DNA片段雜交之菌株，加以鑑定之後，分別命名?Alcaligenes faecalis type II、Alcaligenes faecalis ss faecalis和一株未能鑑定出菌名之Selection yu，探討其在不同酸鹼值、不同電子供給者、不同氧氣含量與不同抑制劑的添加下，對硝化及脫氮作用的影響。並與購買來的Thiosphaera pantotropha、Paracoccus denitrificans、Thiobacillus denitrificans做比較。 Thiosphaera pantotropha在pH 6.5、氧量?大氣氧量的40％、添加10 mM硫酸鈉及氰化鉀時，硝化作用最佳；pH 6.5、氧量?大氣氧量的25％、添加10 mM硫代硫酸鈉及hydrazine時，脫氮效果最好。 Paracoccus denitrificans在pH 7.0、氧量?大氣氧量的30％、添加10 mM硫代硫酸鈉及氰化鉀時，硝化作用最佳；pH 7.5、氧量?大氣氧量的30％、添加20 mM硫代硫酸鈉及hydroxylamine、hydrazine時，脫氮效果最好。 Thiobacillus denitrificans在pH 6.5、氧量?大氣氧量的25％、添加20 mM硫酸鈉時及氰化鉀時，硝化作用最佳；pH 7.5、氧量?大氣氧量的30％、添加10 mM硫酸鈉時及hydroxylamine時，脫氮效果最好。 Alcaligenes faecalis type II在pH 6.0、氧量?大氣氧量的40％、添加10 mM硫酸鈉及hydrazine時，硝化效果最佳；pH 7.0、氧量?大氣氧量的40％、添加20 mM硫代硫酸鈉及hydroxylamine時，脫氮效果最好。 Alcaligenes faecalis ss faecalis在pH 7.0、氧量?大氣氧量的40％、添加20 mM亞硫酸鈉及氰化鉀時，硝化作用最佳；pH 6.0、氧量?大氣氧量的35％、添加20 mM硫代硫酸鈉及hydrazine、hydroxylamine時，脫氮效果最好。 Selection yu在pH 7.0、氧量?大氣氧量的30％、添加20 mM硫酸鈉及氰化鉀時，硝化作用最佳；pH 7.5、氧量?大氣氧量的30％、添加20 mM硫代硫酸鈉及hydrazine、hydroxylamine時，脫氮效果最好。

The studies was trying to isolate bacteria, which could perform nitrification and denitrification, from nitrogen containing wastes from Taiwan. The isolates which could hybridized with the probes made from DNA fragments of Thiosphaera pantotropha, were selected and identified. Two were named Alcaligenes faecalis type II and Alcaligenes faecalis ss faecalis and another unidentified was named Selection yu. The effect of different pH values, electron donors, oxygen contents and inhibitors to nitrification and denitrification of the isolates were studied. We also compare the isolated bacteria with Thiosphaera pantotropha, Paracoccus denitrificans and Thiobacillus denitrificans. When the pH value was adjusted to 6.5, oxygen content adjusted to 40% of air saturation, 10 mM sodium sulfate or potassium cyanide added into the medium, the nitrification rate of Thiosphaera pantotropha was the best. When the pH value was adjusted to 6.5, oxygen content adjusted to 25% of air saturation, 10 mM sodium thiosulfate or hydrazine added into the medium, the denitrification rate was the best. When the pH value was adjusted to 7.0, oxygen content adjusted to 30% of air saturation, 10 mM sodium thiosulfate or potassium cyanide added into the medium, the nitrification rate of Paracoccus denitrificans was the best. When the pH value was adjusted to 7.5, oxygen content adjusted to 30% of air saturation, 20 mM sodium thiosulfate, hydroxylamine or hydrazine added into the medium, the denitrification rate was the best. When the pH value was adjusted to 6.5, oxygen content adjusted to 25% of air saturation, 20 mM sodium sulfate or potassium cyanide added into the medium, the nitrification rate of Thiobacillus denitrificans was the best. When the pH value was adjusted to 7.5, oxygen content adjusted to 30% of air saturation, 10 mM sodium sulfate or hydroxylamine added into the medium, the denitrification rate was the best. When the pH value was adjusted to 6.0, oxygen content adjusted to 40% of air saturation, 10 mM sodium sulfate or hydrazine added into the medium, the nitrification rate of Alcaligenes faecalis type II was the best. When the pH value was adjusted to 7.0, oxygen content adjusted to 40% of air saturation, 20 mM sodium thiosulfate or hydroxylamine added into the medium, the denitrification rate was the best. When the pH value was adjusted to 7.0, oxygen content adjusted to 30% of air saturation, 10 mM sodium thiosulfate, 20 mM sodium sulfate or potassium cyanide added into the medium, the nitrification rate of Alcaligenes faecalis ss faecalis was the best. When the pH value was adjusted to 6.0, oxygen content adjusted to 35% of air saturation, 20 mM sodium thiosulfate, hydroxylamine or hydrazine added into the medium, the denitrification rate was the best. When the pH value was adjusted to 7.0, oxygen content adjusted to 30% of air saturation, 20 mM sodium sulfate or potassium cyanide added into the medium, the nitrification rate of Selection yu was the best. When the pH value was adjusted to 7.5, oxygen content adjusted to 30% of air saturation, 20 mM sodium thiosulfate, hydroxylamine or hydrazine added into the medium, the denitrification rate was the best.