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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | 蕭悅臣 | zh_TW |
dc.date.accessioned | 2021-07-01T08:14:50Z | - |
dc.date.available | 2021-07-01T08:14:50Z | - |
dc.date.issued | 1989 | |
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USA. 84,8498-8501. 12. Welder,J. (1988).Antisense DNA and RNA: progress and prospects. Genes & Development. 2,502-504. 13. Green,N. R. (1989).When the products of oncogenes and anti-oncogenes meet.Cell. 56,1-3. 14. Hudziak,R. M.,Schlessinger,J. and Ullrich,A. (1987) Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells. Proc. Natl. Aced. Sci. USA. 84,7159-7163. 16. Spiegelman,B. M.,Frank,N. and Green,H.(1983).Molecular cloning of mRNA from 3T3 adipocytes. J. B. C. 258,10083-10089. 17. Stuart G. W.,Searle,P. F.,Chen,H. Y.,Brinster,R. L. and Palmiter,R. D. (1984). A 12-base-pair DNA motif tjat is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene. Proc. Natl. Acad. Sci. USA. 81,7318-7322. 18. Davis,L. G.,Dibner,M. D. and Battey,J. F. (1986).Basic methods in molecular biology. 19. Melton,D. A. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75711 | - |
dc.description.abstract | 目前對癌症的研究,已知癌細胞的形成和oncogene有密切的關係,本實驗以B104-1 cell為model利用Antisense RNA techingue去抑制癌細胞的oncogene功能,以期瞭解是否可將癌化細胞轉變為正常的纖維細胞(NIH3T3) .進一步更以Native癌細胞作為觀察對象,是否也能經由抑制其oncogene的功能而轉化為正常細胞. 我們先construct antisense DNA vector,再將antisense vector送入癌細胞,挑選其transfectonts.由southern blot analysis知道Antisense DNA已插入癌細胞的Genomic DNA上.將挑選到的transfectants 做in vitro Biologioal assay(Soft agar assay) ,發現有partially revert現象,另外又發現一種類似revertant cell line.進一步以Native cancer cell做soft agar assay,也發現有partially revert甚至有些transfectant有fully revert回normal cell的現象. | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
dc.description.tableofcontents | 一.中文摘要………………1 二.緒言……………………2 三. 實驗過程簡圖………………7 四.實驗步驟…………………8 五.實驗材枓,試劑和儀器……………13 六.結果…………………18 七.討論………………………32 八. 參考文獻………………………35 九.謝辭………………………………38 | |
dc.language.iso | zh-TW | |
dc.title | 利用反順序核糖核酸技術將癌化的癌細胞轉變?正常的細胞 | zh_TW |
dc.title | Reversion of Cancer Cells to Normal Cells By Antisense-Oncogene | en |
dc.date.schoolyear | 77-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 41 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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