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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 蕭悅臣 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:50Z | - |
| dc.date.available | 2021-07-01T08:14:50Z | - |
| dc.date.issued | 1989 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75711 | - |
| dc.description.abstract | 目前對癌症的研究，已知癌細胞的形成和oncogene有密切的關係，本實驗以B104-1 cell為model利用Antisense RNA techingue去抑制癌細胞的oncogene功能，以期瞭解是否可將癌化細胞轉變為正常的纖維細胞（NIH3T3) ．進一步更以Native癌細胞作為觀察對象，是否也能經由抑制其oncogene的功能而轉化為正常細胞． 我們先construct antisense DNA vector，再將antisense vector送入癌細胞，挑選其transfectonts．由southern blot analysis知道Antisense DNA已插入癌細胞的Genomic DNA上．將挑選到的transfectants 做in vitro Biologioal assay(Soft agar assay) ，發現有partially revert現象，另外又發現一種類似revertant cell line．進一步以Native cancer cell做soft agar assay，也發現有partially revert甚至有些transfectant有fully revert回normal cell的現象． | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 一．中文摘要………………1 二．緒言……………………2 三. 實驗過程簡圖………………7 四．實驗步驟…………………8 五．實驗材枓，試劑和儀器……………13 六．結果…………………18 七．討論………………………32 八. 參考文獻………………………35 九．謝辭………………………………38 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用反順序核糖核酸技術將癌化的癌細胞轉變?正常的細胞 | zh_TW |
| dc.title | Reversion of Cancer Cells to Normal Cells By Antisense-Oncogene | en |
| dc.date.schoolyear | 77-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 41 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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