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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75502完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 舒佩芸 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:33Z | - |
| dc.date.available | 2021-07-01T08:13:33Z | - |
| dc.date.issued | 1984 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75502 | - |
| dc.description.abstract | 本實驗之目的，乃是在培養虎皮蛙之細胞。並研究適合此種細胞生長之環境因數，諸如溫度、滲透壓、犢牛血清濃度等，並就已培養出之各器官細胞，做其外形之觀察與比較，以期建立虎皮蛙之永久細胞株。俾對此種蛙類之病毒診斷研究能有所幫助。 目前已建立虎皮蛙之心臟、腎臟、舌、肺臟、脾臟、肝臟細胞之初級培養，均可在短期（7?11天）內形成密集細胞層。而繼代培養之細胞如心臟細胞已培養至第20代，舌及肺臟細胞已培養至第7?8代，生長情形相當良好。其他腎、脾、肝臟細胞，在初級培養時，細胞生長及□離現象極活?，易形成密集細胞層，但繼代培養後，細胞生長情形不妙，原因不明。 依據所培養之6種不同器官之細胞，發現初級培養細胞多為上皮樣細胞，而繼代培養細胞以紡錘形細胞佔優勢。在掃描式電子顯微鏡下觀察，發現心臟、腎臟、脾臟細胞其表面微絨毛(microvilli)數目極少，有少數較大之泡沫狀突起(blebs)。 虎皮蛙心臟細胞最適宜生長溫度為24?31℃，滲透壓值為150?250mOsm/kg時生長良好。細胞隨培養液之血清濃度增高，生長速率亦隨之變快。在2％及5％犢牛血清之培養液內，則生長極?緩慢，細胞表面附著效應約為13-16％，顯示細胞未變性。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:33Z (GMT). No. of bitstreams: 0 Previous issue date: 1984 | en |
| dc.description.tableofcontents | 一、 摘要------------------------i 二、 謝辭------------------------iii 三、 目錄------------------------iv 四、 內容------------------------1 第一章 緒言------------------------1 第二章 材料與方法------------------------4 第一節 材料------------------------4 2.1-1 虎皮蛙 2.1-2 林格試液 2.1-3 組織洗滌液 2.1-4 胰蛋白?溶液 2.1-5 培養液 2.1-6 不同滲透壓培養液之製備 2.1-7 不同犢牛血清濃度培養液之製備 第二節 方法------------------------9 2.2-1 虎皮蛙細胞之初級培養 2.2-2 繼代培養 2.2-3 溫度對蛙心臟細胞生長速率之影響 2.2-4 滲透壓對蛙心臟細胞生長速率之影響 2.2-5 血清濃度對蛙心臟細胞生長速率之影響 2.2-6 蛙心臟細胞之表面附著效應 2.2-7 細胞大小的測量 2.2-8 掃描式電子顯微鏡之觀察 第三章 結果------------------------15 3-1 虎皮蛙心臟細胞之初級培養及繼代培養 3-2 虎皮蛙腎臟細胞之初級培養及繼代培養 3-3 虎皮蛙舌細胞之初級培養及繼代培養 3-4 虎皮蛙肺臟細胞之初級培養及繼代培養 3-5 虎皮蛙脾臟細胞之初級培養及繼代培養 3-6 虎皮蛙肝臟細胞之初級培養及繼代培養 3-7 溫度對虎皮蛙心臟細胞生長速率之影響 3-8 滲透壓對虎皮蛙心臟細胞生長速率之影響 3-9 血清濃度對虎皮蛙心臟細胞生長速率之影響 3-10 虎皮蛙心臟細胞之表面附著效應 3-11 細胞大小之測量 第四章 討論------------------------24 五、 圖表------------------------29 六、 參考文獻------------------------48 | |
| dc.language.iso | zh-TW | |
| dc.title | 虎皮蛙細胞培養及其特性之研究 | zh_TW |
| dc.title | Studies on the Characteristics of Cell Culture Derived from Tissues of Frog Rana tigerina | en |
| dc.date.schoolyear | 72-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 51 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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