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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75458完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Tschining Chang | en |
| dc.contributor.author | 張俊英 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:18Z | - |
| dc.date.available | 2021-07-01T08:13:18Z | - |
| dc.date.issued | 1982 | |
| dc.identifier.citation | 1. Lee, C.Y.(1979): Adv. Cytophamacology. 3：l-16.
2. Tu, A. T. (1973）: Annu. Rev.Biochem., 42- 3. Lee, C. Y.〔1972): Annu. Rev. Pharmacal., 12: 265-286 4. Dowdall, M. J., Folhman, J.P. and Watts, A. (1979): Adv. Cytophamacology. 3: 63-76。 5. Kelly, R. B., Wedel, R. J. and Strong, P. N. (1979): Adv. cytophamacology，3：77-85. 6. Lee, C. Y., Chang, S. L. and Luh, S. H. (1972): J. Chromatogra., 72: 71-82. 7. Barnard, E. A., Wieckowski, J. and Chiu T. H. (1971): Nature (London), 234: 207-209. 8. Brockes, J. P. and Hall, Z. W. (1975): Biochemistry, 14: 2092-2099。 9. Chang, C. C., Chen, T. F. and Lee, C. Y.(1973）: J. Pharmacol. Exp. Ther., 184: 339-345. 10. Oberg, S. G. and Kelly, R. B. (1976): B. B. A., 433：662-673。 11. MacDremot，J., Westgaard, R. H. and Thompson, E. J. （1978): Biochem. J., 175: 281-288。 12. Lin, C. S.，Wang, C. L. and Blackwell, R. Q. (1975): Toxicon, 13：31-36。 13. Eterovic, V. A. Hebert, M. S. Hanley, M. R. and Bennett E. L. (1975): Toxicon, 13：37-48. 14. Reisfeld R. A. Lewis U. J. and Williams, D. E. (1962): Nature (London), 195: 281-283. 15. Hunter, W. M. and Greenwood, F.C. (1962): Nature (London) 194: 495-498. 16. Gingsborg, B.L. and Warriner, J. (1960): Brit. J. Pharmacol., 15: 410-411. 17. Whittaker, V, P. (1969): in A Lajtha (Editor) Handbook of neurochemistry, 2：327-□. Pienum publishing Corp. New York. 18. Lowry, O. H., Rosebrough， N. J., Farr, A. L. and Randall , R. J. (1951): J. Biol. chem., 193：265-275. 19. Almon, R. R., Andrew, C. G. and Appel, S. H.(1974): Biochemistry, l3：5522-5528。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75458 | - |
| dc.description.abstract | 臺灣雨傘節神經毒素乙，一直是研究神經傳導作用深具潛能的工具之一。由蛇囊取得之粗蛇毒，經由離子交換樹脂分離後，可得15區分。其中第十三區分再由分析法即可得純化之蛇毒神經毒素乙。該毒素可用適當之放射性碘加以標識而不改變其結構與毒性。如此，使得試驗得以在極微量的層次（pmole）進行。在神經毒素對腦神經鍵體作用的研究中，我們發現毒素結合於鍵體的量非常少，而且偵測不到專一結合的現象。因此，神經毒素對神經鍵體的作用必須做更進一步的研究，方能得到明確的答案。 | zh_TW |
| dc.description.abstract | β-BuTx can be modified by chroramine T iodination method, without lose its blocking activity to neuromuscular preparation. With this [125 I] β-BuTx, the binding assay was performed. The total and non specific bound was surprisingly low，and no specific binding was demonstratable. Thus, the toxicity of toxin PLA still remains elusive. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:18Z (GMT). No. of bitstreams: 0 Previous issue date: 1982 | en |
| dc.description.tableofcontents | List of tables List of figures Summary in Chinese Summary in English……………………………………………………………………………1 Introduction……………………………………………………………………………………1 Materials and Methods 1?Materials……………………………………………………………………………………3 2、Instruments………………………………………………………………………………3 3?Methods 3.1 Fractionation of Bungagus multicinctus Venom……………………………………4 3.2 Polyacrylamide gel electrophoresis…………………………………………………5 3.3 Preparation of 125I-labelled β-bungarotoxin……………………………………5 3.4 Structure determination…………………………………………………………………6 3.5 Bioassay on chick biventer cervicis nerve-muscle preparation…………7 3.6 Preparation of synaptosome from guinea pig brain…………………………7 3.7 Protein concentration determination……………………………………………8 3.8 Binding of 125I-labelled toxin to membrane Result…………………………9 Result 1. Fractionation of Venom ……………………………………………………………10 2. Effects of iodotoxin ………………………………………………………………11 3. Binding of 125I-labelled toxin to synaptosome ……………………………22 Discussion………………………………………………………………………………………23 References………………………………………………………………………………………25 Acknowledgement……………………………………………………………………………27 | |
| dc.language.iso | zh-TW | |
| dc.title | 雨傘節神經毒素乙之化學修飾及其對神經鍵體的作用 | zh_TW |
| dc.title | Chemical Modifications of β-Bungorotoxin and ITS Effects On Synaptosome from Guinea Pig Brain | en |
| dc.date.schoolyear | 70-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 34 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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