酵母RNA對泥鰍血紅素及紅血球數量的影響

Abstract

本研究是試驗植物性RNA對於動物體內血紅素及紅血球數量的影晌。所用的材料為酵母RNA及泥鰍Misgurnus anguillicaudatus(CANTOR) 實驗分成三批進行，每次劑量不同，由低濃度至高濃度，腹腔注射，注射量是每尾0.1ml。 第一批，泥鰍140尾，分成五組，第一組為對照組，第二級為實驗對照組，注射0.7% NaCl，共餘三實驗組，分別各注射酵母RNA 0.02, 0.04和0.08mg。 第二批，泥鰍170雙，分成六組，第一組為對照組，第二組為實驗對照組，注射0.7% NaCl，共餘四實驗組，分別注射酵母RNA，0.5,10,15。和2.0mg。 第三批，泥鰍140尾，分成五組，第一組為對照組，第二組為實驗對照組，注射純水，共餘三實驗組，各注射酵母RNA，20，4.0和60mg. 每批實驗，每五天注射及測定一次，連續七次，每次每組各實驗泥鰍四尾，以供測定共血紅素及紅血球數量，測定血紅素用Baush and Lomb Spectronic 20 Colorimeter和Coleman Junior? II Spectrophotometer Model 6/2等二種光電比色計，測定紅血球用血球計數器。 根據測定結果，除了第一批第三組注射酵母0.02mg外，其餘各組經酵母RNA處理者，共血紅素，紅血球數量和對照組此較，均有增加。 第一批，注射酵母RNA 0.04mg者，血紅素增加9.00%，紅血球數量增加8.31％，注射酵母RNA 0.08mg者，血紅素增加6.35%，紅血球數量增加8.00％。 第二批，注射酵母RNA 0.5mg者，血紅素增加4.67%，紅血球數量增加1244%；注射酵母RNA 10mg者，血紅素增加6.28%，紅血球數量增加15.54%；當注射酵母RNA 15mg，血紅素增加3.96%，紅血球數量增加9.27%;注射酵母RNA 2.0mg者，血紅素增加6.11%，紅血球數量增加11.21%,第三批，注射酵母RNA 20mg者，血紅素增加10.99%，紅血球數量增加6.98%；注射酵母RNA 4.0mg者，血紅素增加11.11%，紅血球數量增加9.73%；注射酵母RNA 6.0mg者，血紅素增加9.01%，紅血球數量增加10.28%。經測定共7值，均有顯著性的增加(P:<0.01)。故植物性RNA，在泥鰍體內能參與蛋白質的合成作用，使血紅素增加。

This study was conducted to determine the effects at yeast RWA on the. synthesis of hemoglobin. and RBC count in pond loach Misgurnus anguillicaudatus (CANTOR) The experiment was divided into 3 parts. Every part took the different dosage of yeast RNA. The experimental animals were injected intraperitonialy with 0.1 ml solntion per animal. Part I. 140 of pond 1oack were allotted. into 5 groups by random. Group A was used as the initial eontrol. Group B, the experimental control, was injected with 7% NaCl solution. The other three groups ere inject repectively with 0.02, 0.04 and 0.08 mg/O. lml of yeast RNA. Part II. 170 of pond loach were alloted into 6 groups by random. Group A was used as the initial control. Croup B, the experimental control, injeced with 0.7% NaCl solutio. The other four groups were injected respectively with 0.5, 1.0, 1.5 and 2.0 mg/0.1 ml of ysast A. Part III. 140 of pond loach were allotted into groups by random. Group A was se s the initial control. Group B, the experimental control, was injected with pure water. The other three groups were injected respectively with 2.0, 4.0, and 6.0 mg/0.l ml of yeast RNA in water. All of thee were injected at 5-day interval. Bach five day, 4 Bond loach from each group were Sacrified for determing the hemoglobin and RNA count. The results of these experiments were as followsa: 1. The experiment groups of Bart I were compaced wish initial control, pond leach injected with the doses of 0.04mg and 0.08mg PNA Were found that their hemoglobin concentration were 9.00% and 6.35% higher than the initial control reepetively, and that their RBC court were 8.31% and 8.00% higher than the control, too. 2. Part II. Pond loach injected with the doses of G. S. 1.0, 1.5, and 2.0 mg RNA were found that tmeir hemoglobin concentration were 4.67%, 6.28%, 3.96% and 6.11% higher than the conirol resiectively, and that their RBO count were l2.44% 15.54% 9.27% and 11.21%-higher than the eontrol. 3. Part III, Pond loach injected with the doses of 2.0, 4.0, and 6.0 mg RNA were found that their hemoglobin concentration. were 10.99%, 11.11%,and 9.01% higher then the initial control reonectivesly and their RBC count were 6.98%, and 10.28% higher than the control. 4. The difference between the. experimentel groups and the control denerming by the F test method, was significance in their bemoglobin conoentrition and RBC count.