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標題: | Bcl - 2 細胞凋亡家族基 因轉殖對斑馬魚肝臟器官發育之調控 Apoptotic Bcl-2 family genes regulate the zebrafish liver organogenesis through transgenesis |
作者: | Chih-Hung Cheng 鄭智鴻 |
出版年 : | 2003 |
學位: | 碩士 |
摘要: | 斑馬魚在研究發展上有許多實驗上的優點,雖然在傳統上已經用他們來闡明早期胚發育的機制,但是他們也能夠被善加地利用在較後期的發育上,例如在器官發生的發展方面調查問題。 Bcl - 2 基因群為調控細胞凋亡(apoptosis)進行之基因,而細胞凋亡是脊椎動物早期發育的一個十分重要部分,它涉及在雕塑器官的形狀上,所以當肝臟形成時,促細胞凋亡和抗細胞凋亡之間的平衡被打破將可能導致肝臟過度增殖或者發育不全。而為瞭解肝臟形成的發育過程,利用斑馬魚作為一個模式系統,並使用實驗室中已有的表現綠螢光肝臟之基因轉殖魚,來瞭解過量表現 BLP1 和 Mcl-1a 基因時會對肝臟的器官發生產生如何的影響。從基因轉殖魚分析的結果,發現到過量表現BLP1 和Mcl-1a 基因於斑馬魚幼魚發育期間會導致肝臟的過度生長,並且發現到許多調節生長的基因受到改變,如XBP -1和 C /EBP? 等基因都會增加表現。 Zebrafish embryos have several experimental advantages for studying development. Although they have traditionally been used to elucidate mechanisms of early development, they can also be exploited to investigate issues later in development such as organogenesis. Previous studies have identified genes of the Bcl-2 family as a group of evolutionarily conserved genes that regulate cellular apoptosis. Since apoptosis is an essential part of normal embryonic development in vertebrates, it is involved in sculpturing organs. For instance, interruption of the balance between apoptosis and anti-apoptosis in liver formation may cause liver hyperplasia or hypoplasia. To understand the apoptosis regulated liver formation, we have used the zebrafish as a model to understand the liver organogenesis by overexpressing BLP1 and Mcl-la genes in the liver of transgenic zebrafish, LF2.8-TG1, whose liver could express GFP. Results from the transient transgenic analysis suggest that the zebrafish BLP1 and Mcl-la genes might interrupt the liver formation during zebrafish embryogenesis. Results from our findings in the enlarged liver indicate that overexpression of Bcl-2 family proteins in zebrafish leads to liver overgrowth, and several transcription factors, such as XBP-1 and C/EBP? are up regulated. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75432 |
全文授權: | 未授權 |
顯示於系所單位: | 漁業科學研究所 |
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