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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Huan-Chin Tseng | en |
| dc.contributor.author | 曾煥清 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:59Z | - |
| dc.date.available | 2021-07-01T08:12:59Z | - |
| dc.date.issued | 2003 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75392 | - |
| dc.description.abstract | MpSv-2基因被證實在小鼠的儲精囊表現，其表現受到了雄性素的刺激，而儲精囊是小鼠體內唯一表現MpSv-2基因的器官。MpSv-2基因cDNA序列一共有2733鹼基對，其中包含一個完整的ORF含有2463鹼基對，可以轉錄含有820個胺基酸的蛋白質，推估分子量為90,200 Da。完成MpSv-2基因的基因組序列分析發現，MpSv-2基因一共含有5個 exon及4個intron。在延伸至2002個鹼基的啟動子區域序列中，以電腦軟體分析得到6個可能的雄性素反應單元，l個在啟動子區域、2個在第1個exon中、3個在第2個intron中。 MpSv-2基因所轉譯的蛋白透過電腦分析，發現極有可能是分泌性蛋白，且其估計分子量與小鼠儲精囊分泌性蛋白SVS I很接近，以胰蛋白?分解SVS I後進行MALDI-TOF質譜分析各片段之分子量，證實了SVS I是MpSv-2基因所轉譯的蛋白。由免疫組織染色實驗證實了SVS I是由儲精囊表皮細胞所分泌，並且儲存於儲精囊腔道中。經由轉麩胺醯?所催化的交聯實驗證實，SVS I蛋白可以與SVSⅡ以及SVSⅢ一起被作用而形成高分子聚合物， SVSⅠ蛋白為轉麩胺醯?受質之一。 | zh_TW |
| dc.description.abstract | The genomic structure of MpSv-2, a novel androgen-regulated gene exclusively expressed in mouse seminal vesicle, was analyzed to establish a 5-flanking region of 2002 bp, five exons of 2038、277、 134、139 and 133 bp and 4 introns of 1060、278、240 and 509 bp. The length of MpSv-2 cDNA is 2733bp. The putative MpSv-2 translate protein contains 820 amino aids that sum to give a molecular mass of 90,200 Da. The translate protein was predicted to a secretory protein and the molecular mass of MpSv-2 translate protein is similar to mouse seminal vesicle secretion protein SVS Ⅰ. Trypsin digestion of SVS Ⅰand MALDI-TOF mass spectral analysis supported this protein as derived from MpSv-2. SVSⅠwas immuolocalized to both the epithelium of the primary and secondary folds of the seminal vesicle.All of mouse SVSⅠ-Ⅲ were proven to be substrates of transglutaminase and could be cross-linked readily after the enzyme reaction. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2003 | en |
| dc.description.tableofcontents | 縮寫表 Ⅲ 摘要 Ⅳ Abstract Ⅴ 第一章 緒論 1 1.1 生殖學概論 1 1.2 哺乳類雄性生殖系統 2 1.3 附屬性腺的研究 3 1.3.1 附屬性腺的差異 3 1.3.2 附屬性腺的生理功能 4 1.3.3 附屬性腺的病理研究 4 1.4 前列腺的發育與功能 4 1.5 儲精囊發育與功能 5 1.6 交配栓 7 1.6.1 交配栓的形成 7 1.6.2 交配栓的功能 7 1.7 研究背景 7 1.7.1 本論文研究緣起 8 1.7.2 論文研究重點 8 第二章 材料與方法 9 實驗材料 9 實驗方法 9 2.1 聚合?連鎖反應 9 2.2 洋菜膠體電泳 11 2.3 DNA片段溶離 12 2.4 限制?反應 13 2.5 小量質體抽取 14 2.6 DNA接合反應質體轉型(Transformation) 15 2.7 質體轉型(Transformation) 15 2.9 MpSV-2基因組片段的製備 20 2.10 Total RNA的萃取 22 2.11 RNA品質的檢定 23 2.12 北方點墨法(Northern blotting) 24 2.13 逆轉錄-聚合?連鎖反應(RT-PCR) 27 2.14 表現載體之誘導 28 2.15 SDS-聚丙烯醞胺膠體電泳 29 2.16 Coomassie Blue G-250染色 30 2.17 西方墨點法 30 2.18 製備SVS I抗體 32 2.19 蛋白質定量 32 2.20 免疫組織化學法 33 2.21 Refolding Inclusion body 35 2.22 蛋白質溶液之透析及濃縮 36 第三章 實驗結果 3.1 尋找小鼠pSV-2基因 37 3.2 MpSv-2之基因組序列與結構分析 38 3.3 MpSv-2基因在各器官間的表現 38 3.4 MpSv-2基因在儲精囊中的表現情形 38 3.5 小鼠MpSv-2基因的表現受到雄性素的刺激 39 3.6 小鼠SVS I蛋白是MpSv-2所轉譯的蛋白 39 3.7 小鼠SVS I抗體的製備 40 3.8 小鼠SVS I蛋白在儲精囊表皮細胞表現 40 3.9 小鼠SVS I蛋白參與交聯作用 40 第四章 討論與未來展望 62 4.1 小鼠的儲精囊中也有MpSv-2的基因的表現 62 4.2 小鼠SVS I基因的表現 62 4.3 小鼠SVS I與TGase的作用 62 4.4 未來研究的方向 63 References 65 附錄 | |
| dc.language.iso | zh-TW | |
| dc.title | 小白鼠儲精囊分泌SIS I蛋白之研究 | zh_TW |
| dc.title | Study of mouse SVS I protein in mouse seminal vesicle secretion | en |
| dc.date.schoolyear | 91-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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