Mitomycin-C對雞胚胎內分泌器官組織化學之影響

Abstract

Some preliminary work relating the effect of mitomyein-C en chick embryos-, has been reported from this laboratory. in this investigation two levela of mitomyein-C (10ug/egg or 20ug/egg) were injected into the fertilized White Leghorn eggs wiich had been incubated for five days in the auto-rotating incubator. On the 13th or 19th day of incubation, the embryos were taken out and the endocrine organs such as the hypophyais, thyroid glands, adrenal glands, thymuses as well s the ovaries or testis were dissected, fixed and proeessed for histochomical studies. The results shoved that the higher the dosage of mitomyoin-C injected the more retarded the. development of the embryos end the higher the motility rate, and vies verss. histochenically, functional degression of the abnormal embryos were observed. There was a decrease of the glycogen content in the endocrine organs. There were less changes in the 10ug group than in the 20ug group. Lipid were notably ipoereasd in the hypophymis and-the gonads of the 13th day chick embryos but were decreased in the 19th day. Lipasd. were decreased in both the 19 or 20ug/egg groups. Alkaline phophatase was inereased in both the 10 or 20ug groups. It was observed that there were only item cells in the hypophymis of the 13th day chick embryos, end differenciated cells in the 19th day. The increase in the number of lymphocytes with accompanying decrease in the retieuloeytes. in the thymuses of the treated groups was observed, and there were quickly growth of thymuses from 13th to 19th day. It was concluded that mitomyein-C effected the normal chemical development of the endocrine organs of the chick embryos.