飼料中添加類胰島素成長因數對赤鰭笛鯛稚魚成長之影響

Abstract

赤鰭笛鯛(Lutjanus erythropterus)俗稱紅魚或海雞母，體略成側扁型，在成長上較為緩慢，約需要二年的時間才能達到上市的規格600-1000g。因此本研究以大腸桿菌生產吳郭魚的類胰島素成長因數(Insulin-like growth factor, IGF)添加於飼料中，探討成長之效果。 實驗一：飼料以魚粉為主要蛋白質來源，以每100g飼料無添加、添加含IGF之E.coli粉末0.04g及0.4g共計3組，結果以每100g飼料中添加含IGF之E.coli粉末0.4g組增重率113％及飼料效率88%，效果最佳。 實驗二：飼料以魚粉為主要蛋白質來源，以每100g飼料無添加、添加不含IGF之E.coli粉末0.4g、含IGF之E.coli粉末0.4g、純化的IGF 0.16mg、動物膠包覆純化的IGF 0.16mg、純化的IGF 0.08mg、動物膠包覆純化的IGF 0.08mg共計7組，結果以每100g飼料中添加含IGF之E.coli粉末組0.4g及動物膠包覆純化的IGF 0.16mg組，其增重率260％及252％和飼料效率89％及88％有最佳效果。

Red striped snapper (Lutjanus erythropterus) grow slowly, it about need two years to reach the marketing size 600-1000g. The aim of this study is to evaluate that the effect of Insulin-like growth factor (IGF) of tilapia procreate from recombinant E. coil add to feed of red striped snapper. Experiment one: Fish meal is the main protein source in diets, the three diets separately control, to add IGF contained in E.coli 0.04g, IGF contained in E.coli 0.4g per100g diet. The results of this experiment, the best weight gain 113% and feed efficiency 88% in feed the diet to add IGF contained in E.coli 0.4g per 100g diet. Experiment two: Fish meal is the main protein source in diets, the seven diets separately control, to add no recombinant E.coli, IGF contained in E.coli 0.4g, pure IGF 0.l6mg, with gelatin containing pure IGF 0.16mg, pure IGF 0.08mg, with gelatin containing pure IGF 0.08mg per 100g diet. The results of this experiment, the diets supplemented IGF contained in E.coli 0.4g and with gelatin containing pure IGF 0.16mg per100g diet have the best weight gain 260% and 252%; feed efficiency 89% and 88%.