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Title: | 界面活性劑虛擬微流道之尺寸調控 Dimension Adjustment of Surfactant Formed Virtual Microchannels |
Authors: | Sheng-Jie Jian 簡聖杰 |
Advisor: | 范士岡(Shih-Kang Fan) |
Keyword: | 介電濕潤,虛擬微流道,尺寸調控,界面活性劑,拉伸DNA, Electrowetting,virtual microchannel,dimension adjustment,surfactant,stretch DNA, |
Publication Year : | 2019 |
Degree: | 碩士 |
Abstract: | 本研究利用電控微流體平台上介電濕潤技術作為驅動液體的機制,我們所使用的工作流體為臨界微胞濃度以上之界面活性劑,因為界面活性劑的分子性質,能夠降低液體的表面張力,我們可以在此平台建立一肥皂泡膜,利用介電濕潤改變液體接觸角達到操縱液體的能力,也可利用介電濕潤使兩側蓄液槽液滴壓力改變進而達到尺寸調控的目的。在此,我們得到當兩側蓄液槽液滴壓力相同時,能夠控制肥皂泡膜的寬度,寬度最細能夠達到0.6 μm,而當兩側蓄液槽液滴壓力形成壓力差時,能控制液體流通在此肥皂泡膜中的方向,並且藉由調整壓力差能夠調整液體流動的速度,由於此肥皂泡膜流道不需實體邊界就能形成流道,稱此流道為虛擬微流道。
在含有界面活性劑的溶液中添加入微球、血液細胞或磁珠,通過施加電壓改變虛擬微流道寬度,能夠控制微球及紅血球的通過與否,亦可利用界面活性劑親油端的特性,在環境中添加入矽油,使磁珠在液體中被截取出來,達到純化檢體的目的。進一步在含有界面活性劑的工作流體中添加入經過螢光染劑染色過後的DNA,DNA會隨液體流動並流經過虛擬微流道,藉由虛擬微流道入口前端的流場拉伸梯度,預期能夠觀察到DNA解開原本的纏繞狀態並被拉伸的過程,並利用在工作流體中添加蔗糖以增加DNA在液體中的鬆弛時間,增加從拉伸狀態回復纏繞狀態的時間。 In this study, we use the technique of electrowetting-on-dielectric (EWOD) as the mechanism for driving the liquid on the electromicrofluidic platform. The liquid utilized is a surfactant with the cell concentration above the critical value, because the molecular property of the surfactant can reduce the surface tension of the liquid. We can build a soap bubble film on this platform using the electrowetting phenomenon to change the contact angle of liquid and engendering the ability of manipulating liquid. It is also feasible to dimension adjustment of reservoirs at both ends respectively by the hydraulic pressure difference between the reservoirs that have induced by contact angle change as electrowetting occur. Here we dominate the least width of soap bubble film to be 0.6 μm, as the hydraulic pressure in two reservoirs at both ends are consistent. When the hydraulic pressure between two reservoirs lead to be distinct, we can control the hydraulic pressure by applying voltages to manipulate flow rate and direction of liquid in the soap bubble film. So, we simply formed a so-called virtual microchannel without any physically restricted boundaries. We tried to use different width of virtual microchannel to achieve varying applications by mixing polystyrene microspheres, red blood cells and magnetic beads in liquid with surfactant. Observing the red blood cells and polystyrene microspheres passing through a virtual microchannel can have functions as flow cytometry. Besides with the lipophilic property of surfactant, purifying samples with the virtual microchannel can be fulfilled by infusing silicone oil around when the samples passing through it. Moreover, we add DNA stained fluorescent dye (YOYO-1) into liquid sample to stretch and elongate DNA by the significant gradient of fluidic stress at entrance of virtual microchannel. To improve the effect of observation. Increasing the relaxing time of DNA during elongating state and recovery state. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74974 |
DOI: | 10.6342/NTU201902625 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 機械工程學系 |
Files in This Item:
File | Size | Format | |
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ntu-108-1.pdf Restricted Access | 3.49 MB | Adobe PDF |
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