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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 呂廷璋(Ting-Jang Lu) | |
dc.contributor.author | Kai-Yi Lin | en |
dc.contributor.author | 林凱翊 | zh_TW |
dc.date.accessioned | 2021-06-17T09:09:21Z | - |
dc.date.available | 2024-11-04 | |
dc.date.copyright | 2019-11-04 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-10-16 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74876 | - |
dc.description.abstract | 蜂蜜中含有從蜜源植物傳遞而來的化學物質,具有區分蜂蜜的植物來源的應用價值,因此本研究使用UHPLC-DAD-HESI-Q/Orbitrap高解析質譜儀分別建立蜂蜜中特定目標物與非特定目標物兩套分析方法。特定目標物以蜂蜜中主要的多酚類化合物與離層素為基礎,可檢測14種酚酸、16種類黃酮與2種離層素的組成與含量,研究結果顯示台灣產蜂蜜主要酚酸為protocatechuic acid、4-hydroxybenzoic acid、p-coumaric acid、salicylic acid,主要類黃酮為luteolin。台灣產龍眼蜜中兩種構型的abscisic acids含量皆高於泰國產龍眼蜜,因此具有做為判別來自不同產地龍眼蜜的潛力。非特定目標物分析則藉由高解析質譜儀建構蜂蜜樣品的指紋圖譜,搭配主成分分析進行區分。實驗結果顯示,相較於以特定目標物主成分分析的結果,非特定目標物分析可藉由第二主成分進一步區分來自台灣產龍眼蜜與泰國產龍眼蜜。本研究除了所開發的特定目標物分析方法,有助於了解多酚類化合物與離層素於蜂蜜中的含量與分布外,非特定目標物搭配主成分分析則提供一套區分蜂蜜植物來源,更有效的方法與新的思路。 | zh_TW |
dc.description.abstract | Honey contains chemicals that transferred from the botanical sources through the bee, and these chemicals have application value to discriminate a botanical source of honey. Therefore, this study used UHPLC-DAD-HESI-Q/Orbitrap high-resolution mass spectrometer to establish two sets of analysis methods for targeted and non-targeted analysis in honey. The targeted analysis was based on the main polyphenolics and abscisic acids in honey. There were 14 phenolic acids, 16 flavonoids and 2 forms of abscisic acids that can be determined in targeted analysis. The results showed that the main phenolic acids in Taiwanese honey were protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, salicylic acid, and the main flavonoids was luteolin. The content of abscisic acids in Taiwanese longan honey were higher than Thai longan honey, so it has the potential to discriminate longan honey from different regions. The non-targeted analysis used a high-resolution mass spectrometer to construct fingerprints of the honey samples, which was discriminated against by principal component analysis. The experimental results show that it can further discriminate Taiwanese longan honey from Thai longan honey by the second principal component. In addition to the targeted analysis developed in this study, it is helpful to understand the content and distribution of polyphenolics and abscisic acids in honey. The non-targeted analysis with principal component analysis provides a more effective method and new idea for botanical discrimination of honey. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T09:09:21Z (GMT). No. of bitstreams: 1 ntu-108-R05641014-1.pdf: 12285111 bytes, checksum: 96d2329f73f52864cd9cd0d183e362bd (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝i
摘要ii Abstract iii 目錄I 圖目錄V 表目錄XVI 壹、 前言1 貳、 文獻回顧2 2.1蜂蜜2 2.1.1簡介2 2.1.2製造流程2 2.1.3蜂蜜標準3 2.1.3.1品質標準3 2.1.3.2真實性標準4 2.2蜂蜜成份指標6 2.2.1醣類6 2.2.2胺基酸、蛋白質與酵素9 2.2.3維生素11 2.2.4礦物質11 2.2.5有機酸12 2.2.6香味物質13 2.2.7離層素14 2.2.8多酚類化合物15 2.3多酚類化合物與離層素之分析方法20 2.3.1樣品前處理20 2.3.1.1直接稀釋進樣法20 2.3.1.2液相-液相萃取法20 2.3.1.3固相萃取法22 2.3.2分離方法26 2.3.2.1高效液相層析法26 2.3.2.2毛細管電泳法27 2.3.3檢測方法28 2.4非特定目標物分析30 2.4.1指紋圖譜30 2.4.2數據前處理33 2.4.3多變量分析33 參、 實驗目的與研究架構35 肆、 材料與方法36 4.1實驗材料36 4.1.1蜂蜜36 4.1.2高果糖糖漿37 4.2藥品與試劑37 4.2.1標準品37 4.2.2化學藥品38 4.3儀器設備與軟體40 4.3.1樣品前處理40 4.3.2超高效液相層析串聯質譜儀42 4.3.3軟體43 4.4實驗方法44 4.4.1樣品前處理44 4.4.2多酚類化合物與離層素定量分析46 4.4.3非特定目標物分析51 伍、 結果與討論56 5.1蜂蜜中多酚類化合物與離層素定量分析56 5.1.1蜂蜜樣品前處理56 5.1.2酚酸、類黃酮與離層素定性分析58 5.1.3標準曲線、方法定量極限及回收率66 5.1.4蜂蜜中酚酸、類黃酮與離層素之含量72 5.1.5特定目標物之主成分分析85 5.2蜂蜜之非特定目標物分析88 5.2.1實驗條件之選擇88 5.2.2非特定目標物之主成分分析90 5.2.3未知物鑑定95 陸、 結論98 柒、 參考文獻99 捌、 附錄116 8.1查核樣品滯留時間與濃度品質管制116 8.1.1查核樣品滯留時間品質管制119 8.1.2查核樣品(相對)含量濃度管制125 8.2多酚類與小分子化合物品質管制平台之建立131 8.2.1多酚類化合物與小分子化合物之萃取方法131 8.2.2含蜂蜜多酚類化合物查核樣品原液之配製132 8.2.2.1液體樣品處理流程132 8.2.2.2固體樣品處理流程132 8.2.2.3蜂蜜樣品處理流程132 8.2.2.4含蜂蜜多酚類化合物查核樣品原液配製流程133 8.2.3多酚類化合物與小分子化合物之分離134 8.2.4軌道阱式質譜儀多酚類與小分子化合物鑑定135 | |
dc.language.iso | zh-TW | |
dc.title | 蜂蜜中化合物之特定及非特定目標分析 | zh_TW |
dc.title | Targeted and Non-targeted Analysis for Chemical Compounds in Honey | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張永和,魏國?,駱錫能,楊恩誠 | |
dc.subject.keyword | 蜂蜜,高解析質譜儀,非特定目標物分析,多酚類化合物,離層素, | zh_TW |
dc.subject.keyword | Honey,HRMS,Non-targeted analysis,Polyphenolics,Abscisic acid, | en |
dc.relation.page | 271 | |
dc.identifier.doi | 10.6342/NTU201904211 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-10-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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