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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 郭柏齡(Poling-Kuo) | |
dc.contributor.author | JUI-KANG KUO | en |
dc.contributor.author | 郭瑞崗 | zh_TW |
dc.date.accessioned | 2021-06-17T08:22:47Z | - |
dc.date.available | 2022-08-31 | |
dc.date.copyright | 2019-08-19 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-13 | |
dc.identifier.citation | [1] J. R. Hilton, D. T. Williams, B. Beuker, D. R. Miller, and K. G. Harding. Wound Dressings in Diabetic Foot Disease. Clin Infect Dis, 2004.
[2] Selvaraj Dhivya, Viswanadha Vijaya Padma, and Elango Santhini. Wound dressings – a review. Biomedicine (Taipei), 2015. [3] Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of International Medical Research, 2009. [4] Azar Nourian Dehkordi, Fatemeh Mirahmadi Babaheydari, Mohammad Chehelgerdi, Shiva Raeisi Dehkordi. Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies. Stem Cell Research & Therapy, 2019 [5] Chin Hsiao Tseng. Prevalence and Risk Factors of Diabetic Foot Problems in Taiwan: A cross-sectional survey of non-type 1 diabetic patients from a nationally representative sample. Diabetes Care, 2003. [6] Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringdén O. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol, 2003. [7] Wang L, Wang L, Cong X, Liu G, Zhou J, Bai B, Li Y, Bai W, Li M, Ji H, Zhu D, Wu M, Liu Y. Human umbilical cord mesenchymal stem cell therapy for patients with active rheumatoid arthritis: safety and efficacy. Stem Cells Dev, 2013. [8] Darwin J. Prockop and Joo Youn Oh. Mesenchymal stem/stromal cells (MSCs): Role as guardians of inflammation. Mol Ther, 2012. [9] Hongyan Tao, Zhibo Han, Zhong Chao Han, and Zongjin Li. Proangiogenic Features of Mesenchymal Stem Cells and Their Therapeutic Applications. Stem Cells International, 2016. [10] 林泰元、陳耀昌、黃彥華. (新型第M462274號) 一種可由特定組織中取得成體幹細胞以製成生物製劑系統。中華民國新型專利2013.9.21 ~ 2023.02.20. [11] 林泰元、陳耀昌、侯勝茂、嚴孟祿、黃彥華、吳孟學. (發明第I419971號) 一種成體幹細胞之組織分解、細胞黏附與萃取寄培養技術。中華民國發明專利 2013.12.21 ~ 2031.09.27. [12] Wesley M. Jackson, Leon J. Nesti, and Rocky S. Tuan. Concise Review: Clinical Translation of Wound Healing Therapies Based on Mesenchymal Stem Cells. STEM CELLS Translational Medicine, 2012. [13] Linhao Wang, Fang Wang, Liling Zhao, Wenjun Yang, Xinxing Wan, Chun Yue, and Zhaohui Mo. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats. Stem Cells International, 2019. [14] Juliane Ertl, Melanie Pichlsberger, Alexandru Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H. Geyer, Barbara Maurer-Gesek, Wolfgang J. Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars Peter Kamolz, and Ingrid Lang. Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds. Placenta, 2018. [15] Koehler, J., Brandl, F. P., Goepferich, A. M. Hydrogel wound dressings for bioactive treatment of acute and chronic wounds. European Polymer Journal, 2018. [16] Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Ali Tamayol, Nasim Annabi, and Ali Khademhosseini. Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials, 2015. [17] An I. Van Den Bulcke, Bogdan Bogdanov, Nadine De Rooze, Etienne H. Schacht, Maria Cornelissen, and Hugo Berghmans. Structural and rheological properties of methacrylamide modified gelatin hydrogels. Biomacromolecules, 2000. [18] Mingyue Sun, Xiaoting Sun, Ziyuan Wang, Shuyu Guo, Guangjiao Yu, and Huazhe Yang. Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue. Polymers, 2018. [19] Chang, H. Projector-based 3D printing for pcMSC cell sheets. Master Thesis of Department of Biomedical Electronics and Bioinformatics College of Electrical Engineering and Computer Science. National Taiwan University. 4-21pp. [20] Monica M. Laronda, Alexandra L. Rutz, Shuo Xiao, Kelly A. Whelan, Francesca E. Duncan, Eric W. Roth, Teresa K. Woodruff, and Ramille N. Shah. A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. Nature Communications, 2017. [21] Yanen Wang, Kai Wang, Xinpei Li, Qinghua Wei, Weihong Chai, Shuzhi Wang, Yu Che, Tingli Lu, and Bo Zhang. 3D fabrication and characterization of phosphoric acid scaffold with a HA/β-TCP weight ratio of 60:40 for bone tissue engineering applications. PLoS ONE, 2017. [22] Ali Bagheri and Jianyong Jin. Photopolymerization in 3D Printing. ACS Applied Polymer Materials, 2019. [23] Kakasheva-Mazhenkovska L, Milenkova L, Gjokik G, Janevska V. Variations of the histomorphological characteristics of human skin of different body regions in subjects of different age. Prilozi, 2011. [24] ISO 10993-5:2009(E) Biological evaluation of medical devices — Part 5: Tests for in vitro cytotoxicity [25] ISO 10993-12:2012(E) Biological evaluation of medical devices — Part 12: Sample preparation and reference materials | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74170 | - |
dc.description.abstract | 間質幹細胞擁有高度的免疫調節以及再生能力,也因此近幾年來間質幹細胞一直都是細胞治療的主力人選,至今也已有眾多臨床試驗證實間質幹細胞在傷口癒合的過程中有良好的表現。然而間質幹細胞促進傷口的癒合的機制以及怎樣給予細胞才能達到最好的效果還尚未有一個完整的解釋。這份研究中我們提出多種細胞治療傷口貼布好讓細胞可以在治療期間停留在傷口處,這些以明膠為基底的傷口貼布透過3D列印技術可以客製化的製作出各種形狀,以配合各種不同的臨床需求。細胞在這樣的貼布裡面能夠維持活性超過12小時,這樣的保存條件也讓細胞貼布在未來有機會變成一個令人期待的新興產品。 | zh_TW |
dc.description.abstract | Mesenchymal stem cells (MSCs) have been shown to be potential candidates to cell therapy due to their immunomodulation and regeneration abilities. Lots of clinical trials have reached successful results that MSCs could enhance the wound healing process. However, the mechanism how MSCs affect the process and what is the most efficient way to apply cells to the wound still need to be investigated. Some novel cell-embedded patches are introduced in this study to keep the cells at the wound site. The GelMA-based wound healing patch can be fabricated on customization to any shape to meet the clinical needs through 3D printing technique. Most of the cells could survive for more than 12hrs under proper condition, which made the patch a promising product for clinical use in the near future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:22:47Z (GMT). No. of bitstreams: 1 ntu-108-R05945004-1.pdf: 5520137 bytes, checksum: 7f5ce2ad458d2a2bbeeee68707211ad0 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iii Content iv Chapter 1 Introduction 1 Chapter 2 Materials and Methods 8 2.1 GelMA fabrication 8 2.2 3D printing material preparation 9 2.3 3D printing 10 2.3.1 3D printer setup 10 2.3.2 line width 11 2.3.3 Logo and customized shape printing 12 2.3.4 3D structure 14 2.3.5 Shape-changing property study 15 2.4 Stem-cell-embedded wound healing patch fabrication 16 2.4.1 Cell culture 16 2.4.2 GelMA film 16 2.4.3 Double-layer hexagon grids patch fabrication 17 2.4.4 In-situ GelMA patch 18 2.4.5 Sandwich-patch 19 2.4.6 Fishbone GelMA chain fabrication 20 2.5 Manufacture of handheld UV LED light source 21 2.6 Cell viability test 23 2.7 GelMA cytotoxicity 24 2.7.1 direct contact sample preparation and MTT assay 24 2.7.2 extract sample preparation and MTT assay 25 2.8 GelMA decomposition test 26 2.8.1 MMP2 inhibitor 26 2.8.2 protease inhibitor cocktail 28 2.9 Animal testing 29 Chapter 3 Results and discussion 30 3.1 3D printing 30 3.1.1 Line width 30 3.1.2 Logo, customized shape, and 3D structure printing 33 3.1.3 Shape-changing property 36 3.2 Stem-cell embedded patch and cell viability 38 3.2.1 GelMA film 38 3.2.2 Double-layer hexagon grids patch 40 3.2.3 In-situ GelMA patch 43 3.2.4 Sandwich patch 45 3.2.5 Fishbone GelMA chain 48 3.2.6 pcMSC maintain high viability in the patches 50 3.3 GelMA cytotoxicity evaluation 51 3.3.1 Sample extract test 51 3.3.2 Raw material direct contact test 53 3.4 GelMA decomposition test 55 3.4.1 MMP2 inhibitor 55 3.4.2 protease inhibitor cocktail 58 3.5 Animal testing 60 Chapter 4 Conclusion 65 Reference 66 | |
dc.language.iso | en | |
dc.title | 光聚合3D列印幹細胞傷口貼布 | zh_TW |
dc.title | A novel stem-cell-embedded wound healing patch fabricated by photopolymerization 3D printing | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 趙本秀,黃彥華 | |
dc.subject.keyword | 間質幹細胞,胎盤蛻膜間質幹細胞,甲基丙烯?基明膠,3D列印,傷口癒合, | zh_TW |
dc.subject.keyword | Mesenchymal stem cells (MSCs),pcMSC,GelMA,3D printing,wound healing patch, | en |
dc.relation.page | 68 | |
dc.identifier.doi | 10.6342/NTU201902810 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-14 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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