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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝淑貞(Shu-Chen Hsieh) | |
dc.contributor.author | Wei-Ting Tai | en |
dc.contributor.author | 戴暐庭 | zh_TW |
dc.date.accessioned | 2021-06-17T08:17:22Z | - |
dc.date.available | 2019-08-18 | |
dc.date.copyright | 2019-08-18 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-14 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74030 | - |
dc.description.abstract | 代謝症候群是指血壓或空腹血糖偏高、血脂異常、腹部肥胖等許多與糖尿病和心血管疾病相關的危險因子聚集的現象,主要與生理代謝異常而產生胰島素阻抗的情形有關,而造成胰島素阻抗的原因主要為肥胖。肥胖為攝取高熱量食物以及運動量不足所導致,不過近年來也發現腸道菌相在其中扮演重要角色,而許多研究發現環境中許多物質亦有導致肥胖的疑慮,例如農藥、多氯聯苯、雙酚A等。本研究以使用量較大與使用較廣泛之殺蟲劑陶斯松作為主軸,以一般飲食與高脂飲食小鼠為模式,探討國人飲食中陶斯松的暴露量是否會影響其脂肪組織中脂質合成、代謝以及慢性發炎的反應,亦探討其對於腸道菌相的影響。在一般飲食及高脂飲食下,給予小鼠不同劑量陶斯松持續90天,結果顯示在高脂飲食下低劑量陶斯松對於皮下脂肪組織的脂質堆積較控制組有顯著影響,而腸道菌相的資訊顯示,各組之Firmicutes與Bacteroidetes ratio組間無顯著差異,低劑量和中劑量則可減少Deferribacteres的比例;低劑量亦會減少Verrucomicrobia的比例,脂多醣(lipopolysaccharide, LPS)的數據則顯示本研究所使用的劑量可能尚未影響腸道通透性,此外也以3T3-L1細胞模式探討陶斯松對脂肪細胞脂肪之影響,結果顯示低劑量陶斯松對脂肪細胞分化以及脂解作用的影響明顯高於高劑量處理,綜合上述,本研究發現低劑量的陶斯松對於動物體與脂肪細胞的影響,其相關機制仍有待後續研究進行確認。 | zh_TW |
dc.description.abstract | Metabolic syndrome is a cluster of conditions, including increased blood pressure, high fasting blood sugar, abnormal cholesterol or triglyceride levels, excess body fat around the waist occurs together increasing your risk of heart disease and type 2 diabetes. Main reason of metabolic syndrome is insulin resistance due to metabolic abnormality. Then obesity always leads to insulin resistance. High-density caloric diets coupled with decreased physical activity as the root causes of obesity while the latest researches also regard gut microbiota as crucial factor. However, several studies have indicated the remaining chemical compound in our environment is also the possibility reason of obesity, for example pesticide, polychlorinated biphenyl (PCB), bisphenol A and so on. The aim of this study was to explore the Taiwanese dietary exposure of large usage amount, widely used pesticide-chlorpyrifos whether to affect fat synthesis and metabolism, also the grade of chronic inflammation in adipose tissue and the composition of gut microbiota by normal diet and high fat diet mice model during 90 days. Results showed that weight of inguinal subcutaneous fat in low dose chlorpyrifos group significantly higher than control group. The gut microbiota abundance ratio of phylum Firmicutes and Bacteroidetes didn’t have significantly difference between control and various doses chlorpyrifos group, but it was decreased abundance of Deferribacteres in low dose and medium dose. Abundance of Verrucomicrobia also decreased in low dose. Regardless of diet conditions, the lipopolysaccharide (LPS) concentration in serum didn’t have significantly difference between control and various doses chlorpyrifos group. It could show that dietary exposure chlorpyrifos didn’t affect the intestinal integrity. Furthermore, to investigate the effect of chlorpyrifos in adipocyte by 3T3-L1 cell model. Results showed that low dose significantly inhibited the adipogenesis and lipolysis than control group. In sum, this study found that the effects of low doses of chlorpyrifos on animal and adipocytes, the relevant mechanism remains to be confirmed by future studies. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:17:22Z (GMT). No. of bitstreams: 1 ntu-108-R06641038-1.pdf: 3996666 bytes, checksum: 312a76374fb7d80e201e56547bc5c0ca (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 目錄
謝誌 I 摘要 II Abstract III 表目錄 VIII 第一章 前言 1 第二章 文獻回顧 2 第一節 肥胖 2 2.1.1 肥胖在全球與臺灣的盛行率 2 2.1.2 肥胖、代謝症候群與胰島素阻抗 2 2.1.3 肥胖、慢性發炎與胰島素阻抗 3 2.1.4 環境致胖因子(environmental obesogens) 3 第二節 脂肪組織 4 2.2.1 脂肪組織的基本結構與功能 4 2.2.2 脂肪新生(adipogenesis) 4 2.2.3 脂肪分解(adipolysis) 5 2.2.4 脂肪組織與發炎反應 5 第三節 腸道菌 6 2.3.1 人體內腸道菌的基本介紹 6 2.3.2 腸道菌與能量代謝 7 2.3.3 腸道菌失衡(dysbiosis)與代謝異常 7 第四節 農藥-陶斯松介紹 8 2.4.1 陶斯松簡介與應用 8 2.4.2 陶斯松對生理代謝的影響 8 2.4.3 陶斯松對腸道菌相的影響 9 2.4.4 國人飲食中陶斯松的暴露量 10 第三章 研究目的與實驗架構 10 第一節 研究目的 10 第二節 實驗架構-動物實驗 11 第三節 實驗架構-細胞實驗 12 第四章 實驗材料與方法 12 第一節 實驗材料 12 4.1.1 細胞培養 12 4.1.2 實驗藥品 13 4.1.3 實驗試劑與套組 14 4.1.4 實驗耗材 14 4.1.5 實驗設備 15 第二節 實驗方法 15 4.2.1 動物飼養 15 4.2.2 小鼠血糖測定 16 4.2.3 小鼠血清內脂多醣(Lipopolysaccharide, LPS)含量測定 16 4.2.4 小鼠血清胰島素含量測定 17 4.2.5 小鼠脂肪組織石蠟包埋與H&E染色 17 4.2.6 小鼠糞便採取與DNA萃取 20 4.2.7 聚合酶連鎖反應(polymerase chain reaction, PCR) 21 4.2.8 洋菜膠體電泳(agarose gel electrophoresis) 21 4.2.9 小鼠腸道菌相分析 23 4.2.10 3T3-L1細胞株培養與分化 23 4.2.11 細胞存活率測定(MTT assay) 24 4.2.12 油紅染色法(Oil Red O assay) 24 第三節 統計 25 第五章 實驗結果 25 第一節 動物實驗 25 第二節 細胞實驗 29 第六章 討論 31 第一節 一般飲食組 31 第二節 高脂飲食組 34 第三節 細胞實驗、代謝物與劑量相關文獻討論 36 第七章 結論 39 圖表 40 參考文獻 65 | |
dc.language.iso | zh-TW | |
dc.title | 以小鼠為模式探討國人飲食中陶斯松暴露量對肥胖與腸道菌相之影響 | zh_TW |
dc.title | Effects of Taiwanese dietary chlorpyrifos exposure levels on obesity and gut microbiota in normal and high-fat diet-fed mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃智興(Tze-Sing Huang),郭靜娟(Ching-Chuan Kuo),陳億乘(Yi-Chen Chen) | |
dc.subject.keyword | 農藥,陶斯松,肥胖,腸道菌,脂肪新生,脂肪分解, | zh_TW |
dc.subject.keyword | pesticide,chlorpyrifos,obesity,gut microbiota,adipogenesis,lipolysis, | en |
dc.relation.page | 74 | |
dc.identifier.doi | 10.6342/NTU201903333 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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檔案 | 大小 | 格式 | |
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ntu-108-1.pdf 目前未授權公開取用 | 3.9 MB | Adobe PDF |
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