研發含奈米氟幾丁聚醣抗菌複合樹脂作為溝隙封填劑

Abstract

此研究乃利用 離子凝膠法結合氟化鈉與幾丁聚醣以合成 奈米氟幾丁聚醣 ，並加入樹脂基質中，形成 一種既能抗菌又能釋放氟離子的溝隙封填劑。 本研究包括六個部分，第一個部分製備出 奈米氟幾丁聚醣 和奈米級矽烷化二氧化矽的樣品，與雙酚 A 甲基丙烯酸縮水甘油酯 、 三乙二醇二甲基丙烯酸酯 、樟腦醌 、 對二甲胺基苯甲酸乙酯完成混合，製成 奈米氟幾丁聚醣 樹脂。第二個部分是以傅立葉紅外線光譜儀偵測加氟前後幾丁聚醣的結構變化和粒徑大小，發現奈米氟幾丁聚醣 的結構中除了有 1567 cm-1 和 1417 cm-1 的特徵鋒，還多了 738 cm-1顯示三氟甲基形成的可能性，且其粒徑大小為 629 ± 55 nm 。第三部分為利用吸光值和菌落形成單位比較 0% 、 2% 、 4% 的 奈米氟幾丁聚醣樹脂與 Clinpro TM 溝隙封填劑的抗菌能力，結果 2% 奈米氟幾丁聚醣 樹脂的菌落形成單位為控制組的10%, 4%奈米氟幾丁聚醣樹脂的菌落形成單位為控制組的25%，兩者的抗菌效果無顯著差異，但相較於 Clinpro TM 溝隙封填劑有更明顯的抗菌效果。第四部份為觀察不同含量的奈米氟幾丁聚醣萃取液的細胞存活率 、 細胞毒性的高低，發現4% 奈米氟幾丁聚醣 樹脂萃取液有影響細胞複製的能力，故最後選擇 2% 奈米氟幾丁聚醣樹脂 與 Clinpro TM 溝隙封填劑比較機械性質。第五個部分是比較奈米氟幾 丁聚醣 樹脂與市售產品之間的機械性質， 2% 奈米氟幾丁聚醣樹脂的聚合深度 、表面抗壓強度 、 徑向抗拉/壓強度皆高於 Clinpro TM 溝隙封填劑。抗彎強度方面沒有差異性，流動性方面 較差。最後是進行氟離子釋放與再吸收的實驗，發現 2%奈米氟幾丁聚醣樹脂有與市售產品同樣優良的氟離子釋放與再吸收能力。藉由以上的研究結果發現， 2% 奈米氟幾丁聚醣樹脂具有抗菌能力 、 生物相容性 、 良好的機械性質，能釋放與再吸收氟離子， 具有成為溝隙封填劑的潛力。

The purpose of this study was to develop composite resin containing chitosan fluoride nanoparticle (CF) as pit and fissure sealant . It had the antibacterial characteristics and the ability of fluoride release and recharge. There are six parts in this study. First, to prepare composite resin containing CF and silanized silica nanoparticle , bisphenol A glycerolate dimethacrylate , triethylene glycol dimethacrylate, camphorquinone and ethyl 4-dimethylaminobenzoate were mixed with the filler as pit and fissure sealant. Second, we used fourier transform infrared spectroscopy and particle size distribution analyzer to observe the structure and hydrodynamic radius of CF . We found that besides the characteristic peaks, 1567 cm-1 and 1417 cm-1 , there was an additional peak, 738 cm-1 suspected to be the CF3 group. And the hydrodynamic radius was 629 ± 55 nm. Third, the antibacterial activity of resin containing 0%, 2%, 4% CF were compared with Clinpro TM fissure sealant through the measurement of optical density and colony forming units (CFUs).The CFU ratio of composite resin containing 2% CF was 10% compared to the control group, and composite resin containing 4% CF was 25%, which had no significant difference. But both of them had obviously antibacterial activity compared to Clinpro TM fissure sealant. In the fourth part, we observed the cell viability and toxicity of Clinpro TM fissure sealant and resin containing different concentration of CF. We found that the extract of composite resin containing 4% CF could result in the reduction of cell proliferation. Therefore, we choose composite resin containing 2% CF to compare the mechanical properties with Clinpro TM fissure sealant. In the fifth part, we found the value of curing depth, surface hardness, and diametral tensile strength of composite resin containing 2% CF were higher than Clinpro TM fissure sealant, but there was no difference in the flexural strength and the flowability was comparatively lower. Last, the fluoride release and recharge of comparatively lower. Last, the fluoride release and recharge of composite resin containing 2% CF were higher than Clinpro TM fissure sealant, but there was no difference in the flexural strength and the flowability was comparatively lower. Last, the fluoride release and recharge of composite resin containing 2% CF and Clinpro TM fissure sealant were tested. And we found the ability of fluoride release and recharge of composite resin containing 2% CF was as good as Clinpro TM fissure sealant. From the results above, composite resin containing 2% CF could be a pit and fissure sealant, which had the antibacterial activity, biocompatibility, good mechanical strength, and the ability of fluoride release and recharge.