具電荷調節多孔球粒子之振盪電泳行為

Abstract

本研究以具電荷調節多孔球模型為基礎，探討其振盪電泳行為，以提供更趨真實的模擬系統。多孔球模型為模擬聚電解質的重要模型，而聚電解質有很廣泛的應用價值，如許多生物粒子DNA、RNA和蛋白質等，因此在生物醫學領域常見其重要應用，如DNA疫苗、基因轉殖(gene delivery)、藥物釋放…等。因此多孔球粒子的研究為相當重要的課題！ 振盪電泳為測量粒子性質的重要工具，因為相較於一般直流電泳，除了待測物不需要稀釋之優勢外，還多了頻率之調控參數，更可避免焦耳熱效應的產生，降低實驗誤差。 具電荷調節多孔球模型相較於粒子帶電量固定的假設更為真實，因為大多數聚電解質都具有可解離之官能基。本研究會討論電荷調節現象對於振盪電泳動度的影響，主要探討的參數為：電解質離子強度、官能基電荷密度、官能基解離常數、多孔球摩擦係數、外加交流電場頻率、pH值等，以提供實驗學者參考。

We use the charge-regulated porous particle model as the basis to investigate the electrokinetic behavior in dynamic electrophoresis of colloid particle. Many bioparticles such as potein, DNA and RNA are belonged to polyelectrolyte which has great application in biomedical field and is well modeled as charged porous spheres. So it is important to know the behavior of these particles. Dynamic electrophoresis is an important tool for measuring particle properties. Compared with electrophoresis, dynamic electrophoresis can be operated with concentrated sample. Moreover, dynamic electrophoresis is driven by alternating current (AC), which has advantage of convenience of measure and prevention of Joule heating effect. The charge-regulated porous particle model compared with the constant fixed charged porous particle model is more closed to the reality. Since the most polyelectrolytes have dissociable dunctional group on surface, the charge on them are inconstant. In our study, we will discuss the influence of charge-regulation phenomena to the dynamic mobility. Double-layer thickness, functional charge density, friction coefficient, dissociation constant, pH of solution and the electric-field frequency.