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Title: | 癌症衍生之胞外小體對於TH17細胞的作用 The effect of Tumor-derived Exosomes on TH17 cells |
Authors: | Min Chen 陳名 |
Advisor: | 繆希椿(Shi-Chuen Miaw) |
Keyword: | 癌症衍生之胞外小體,黑色素瘤,癌症轉移,TH17輔助型細胞,可塑性, Tumor-derived exosomes,melanoma,metastasis,TH17,plasticity, |
Publication Year : | 2018 |
Degree: | 碩士 |
Abstract: | 目前已知癌症細胞分泌出的胞外小體(exosomes)會幫助血管新生、凝血機制、調控免疫系統,以及改造週邊的薄壁組織(parenchymal tissue),綜合上述功能,這些胞外小體就具有幫助癌症發展的能力。故在此研究中,我們要探討同為黑色素瘤(melanoma)細胞株的B16-F1以及B16-F10兩者之間的癌症轉移能力是否與胞外小體內容物有關。另一方面,TH17細胞的主要功能為引導更多的免疫細胞到癌症細胞或是病原體所在處,以及事後的組織修復。然而,過去研究已知TH17細胞的可塑性(plasticity)可使其轉變(reprogramming)成其他的輔助型T細胞,甚至能轉變成幫助癌症生長的Treg細胞。因此,進一步我們要證實由B16-F1以及B16-F10產生的胞外小體是否導致TH17細胞轉變成Treg細胞。在本研究中,我們首先分離出B16-F1以及B16-F10產生的胞外小體,並且確認他們的大小和表面蛋白符合胞外小體。再進一步,我們將分離出的B16-F1以及B16-F10胞外小體加入正在分化為TH17細胞的naïve T細胞中,我們發現兩種來源的exosomes隨著施加的exosomes蛋白濃度提高至20 g/ml,皆不影響TH17細胞的可塑性。 Tumor-derived exosomes can promote angiogenesis and coagulation, modulate the immune system, and remodel surrounding parenchymal tissue, which together support tumor progression. Therefore, we aimed to characterize the content within the tumor-derived exosomes from B16-F1 and B16-F10, which are known to have different metastasis potential. Furthermore, TH17 cells are responsible for recruiting immune cells against cancer cells or pathogens, and tissue repairing after pathogen clearance. However, TH17 cells are known to have the plasticity to reprogram into other T helper subtypes, for example, Treg cells, which are pro-tumor growth cells. Therefore, we further examined whether B16-F1/F10 exosomes reprogram TH17 cells into Treg cells. Hence, we first isolated and characterized the size and surface proteins of exosomes by Nanoparticle tracking analysis (NTA) and Western blot, respectively. Furthermore, we cultured TH17 cells with isolated B16-F1- and B16-F10-derived exosomes, and identified the TH17 plasticity by quantitative PCR analysis. The results showed that both B16-F1- and B16-F10-derived exosomes do not affect the expression of TH17 related genes with the concentration of exosome protein up to 20 μg/ml. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72337 |
DOI: | 10.6342/NTU201803397 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 免疫學研究所 |
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ntu-107-1.pdf Restricted Access | 1.97 MB | Adobe PDF |
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