耐熱番茄品系CLN1621柱頭外突性狀的遺傳分析

Abstract

CLN1621是一種耐熱番茄品種，在高溫下顯現柱頭外突的性狀。本研究採用遺傳定位的策略分析造成CLN1621品種柱頭外突表現型的遺傳因子。由CA4和CLN1621雜交產生的F7重組自交系用於收集在熱逆境和正常生長條件下的外表型和基因型資料。數量性狀基因座分析結果顯示在熱逆境生長條件下的四個重複試驗中皆辨識到相同的柱頭外突數量性狀基因座。該數量性狀基因座位於第一對染色體32.4 cM位置，並與單核苷多型性分子標記1_3048786緊密連鎖。柱頭外突數量性狀基因座可歸因於高溫影響花柱長度變化的數量性狀基因的作用。

CLN1621, a heat-tolerant tomato cultivars, shows stigma exsertion phenotype at high temperature. The current study deployed the genetic mapping strategy to characterize the genetic components responsible for the stigma exsertion phenotype in the CLN1621 cultivar. The F7 recombinant inbred lines from the cross of CA4 and CLN1621 were used to collect phenotypic data and genotypic data under heat stress and normal growth conditions. The quantitative trait locus (QTL) analysis consistently identified the same stigma exserted QTL in four replicated trials under heat stress growth condition. This QTL is closely linked with the SNP marker 1_3048786 and is located at 32.4 cM on chromosome 1. The effect of this stigma exsertion QTL is attributed to the QTL controlling variation of style length by high temperature.