以紅血球細胞膜覆蓋磁性奈米粒子並結合化學治療與熱治療

Abstract

癌症在現今治療中伴隨著很多副作用，為了解決此問題，奈米粒子已被廣泛研究治療腫瘤。在理想的藥物傳輸系統下，奈米粒子應該具有高生物相容性並且能在血液中有長時間循環，在利用高滲透長滯留效應(EPR effect)累積在腫瘤位置增強治療腫瘤效果。在此研究中，利用兩高分子mPEG-PCL作為奈米載體包載化療藥物SN38與超氧化鐵USPIO。此外使用天然的紅血球細胞膜修飾奈米微胞(micelles)表面，利用紅血球細胞膜表面特定蛋白質特性，可以躲避巨噬細胞吞噬與網狀內皮系統(RES system)，達到延長血液循環時間效果。透過大小、電位，去鑑定RBC-micelles性質。

Clinically, there are many effective cancer chemo drugs, but they always come with adverse effects due to their non-specific biodistribution. One of the effective approaches for tumor-specific accumulation is using nanoparticle formulation based on the theory of enhanced permeation and retention (EPR) effect. To further enhance tumor accumulation, nanoparticles should be kept in blood with little possibility of being eliminated by reticuloendothelial system (RES). In this study, with the intention of achieving ideal drug delivery in cancer treatment, a theranostic micelle loaded with the chemo drug, SN38 and ultrasmall superparamagnetic iron oxide particles (USPIO) for near infrared irradiation was created and camouflaged with red blood cell (RBC) membrane to prevent from uptake by macrophage and RES. Its structure, size, zeta potential, and the content of RBC-membrane were verified using transmission electron microscopy (TEM), dynamic light scattering (DLS).