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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈立言(Lee-Yan Sheen) | |
dc.contributor.author | Eric TeYu Chen | en |
dc.contributor.author | 陳德育 | zh_TW |
dc.date.accessioned | 2021-05-17T09:20:42Z | - |
dc.date.available | 2013-03-19 | |
dc.date.available | 2021-05-17T09:20:42Z | - |
dc.date.copyright | 2012-03-19 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-02-20 | |
dc.identifier.citation | Agelink, M.W., Andrich, J., Postert, T., Wurzinger, U., Zeit, T., Klotz, P., Przuntek, H. (2001). Relation between electroconvulsive therapy, cognitive side effects, neuron specific enolase, and protein S-100. J Neurol Neurosurg Psychiatry 71, 394-396.
Bolstad, B.M., Irizarry, R.A., Anstrand, M., and Speed, T.P. (2003). A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185-193. Borsini, F., and Meli, A. (1988). Is the forced swimming test a suitable model for revealing antidepressant activity? Psychopharmacology (Berl.) 94, 147-160. Brambilla, P.; Clipriani, A.; Hotopf, M., and Barbui, C. (2005). Side-effect profile of fluoxetine in comparison with other SSRIs, trycyclic and newer antidepressants: a meta-analysis of clinical trial data. Pharmacopsychiatry 38, 69-77. Bremner, J.D., Narayan, M., Anderson, E.R., Staib, L.H., Miller, H.L., and Charney, D.S. (2000). Hippocampal volume reduction in major depression. Am J Psychiatry 157, 115-117. Bwalya, G.M., Srinivasan, V., and Wang, M. (2011). Electroconvulsive therapy anesthesia practice patterns: results of a UK postal survey. J ECT 27, 81-85. Chen, P.J., Hsieh, C.L., Su, K.P., Hou, Y.C., Chiang, H.M., Lin, I.H., and Sheen, L.Y. (2008). The antidepressant effect of Gastrodia elata Bl. on the forced-swimming test in rats. Am J Chin Med 36, 95-106. Cheng, A.T. (1995). Mental illness and suicide. A case-control study in east Taiwan. Arch. Gen. Psychiatry 52, 594-603. Clayton, A.H., and Montejo, A.L. (2006). Major depressive disorder, antidepressants, and sexual dysfunction. J Clin Psychiatry 67, 33-37. Cryan, J.F.; Page, M.E., and Lucki, I. (2005). Differential behavioural effects of the antidepressants reboxetine, fluoxetine, and moclobemide in a modified forced swim test following chronic treatment. Psychopharmacology (Berl.) 182, 335-344. Cryan, J.F.; Valentino, R.J., and Lucki, I. (2005). Assessing substrates underlying the behavioural effects of antidepressants using the modified rat forced swimming test. Neurosci. Biobehav. Rev. 29, 547-569. Czeh, B., Michaelis, T., Watanabe, T., Frahm, J., de Biurrun, G., van Kampen, M., Bartolomucci, A., and Fuchs, E. (2001). Stress-induced changes in cerebral metabolites, hippocampal volume, and cell proliferation are prevented by antidepressant treatment with tianeptine. Proceedings of the National Academy of Sciences of the United States of America 98, 12796-12801. Datto, C.J. (2000). Side effects of electroconvulsive therapy. Depress Anxiety 12, 130-134. Dehmelt, L. (2004). Actin and microtubules in neurite initiation: Are MAPs the missing link? Journal of neurobiology 58, 18-33. Depression. (2011). NIMH. http://www.nimh.nih.gov/health/topics/depression/ Depression. (2011). WHO. http://www.who.int/topics/depression/en/ Detke, M.J., and Lucki, I. (1996). Detection of serotonergic and noradrenergic antidepressants in the rat forced swimming test: the effects of water depth. Behav. Brain Res. 73, 43-36. Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). (2011). Allpsych Online. http://allpsych.com/disorders/dsm.html D'Sa, C., Duman, R.S. (2002). Antidepressants and neuroplasticity. Bipolar Disorders 4, 183-194. Duman, R.S., and Monteggia, L.M. (2006). A neurotrophic model for stress-related mood disorders . Elsevier 59, 1116-1127. Duncan, B.L., and Miller, S.D. (2000). Nefazodone, psychotherapy, and their combination for chronic depression. N Engl J Med 343, 1042; author reply 1042-1043. Eriksson, P.S., Perfilieva, E., Bjork-Eriksson, T., Alborn, A.M., Nordborg, C., Peterson, D.A., and Gage, F.H. (1998). Neurogenesis in the adult human hippocampus. Nature medicine 4, 1313-1317. Falloon, I.R. (2000). Problem solving as a core strategy in the prevention of schizophrenia and other mental disorders. Aust N Z J Psychiatry 34 Suppl, S185-190. Fisar, Z., and Hroudova, J. (2010). Common aspects of neuroplasticity, stress, mood disorders and mitochondrial functions. Act Nerv Super Rediviva 52, 3-20. Ferrill, M.J., Kehoe, W.A., and Jacisin, J.J. (1992). ECT During Pregnancy: Physiologic and Pharmacologic Considerations. Convuls Ther 8, 186-200. Gomez, G.E. (2004). Electroconvulsive therapy: present and future. Issues in Mental Health Nursing 4, 473-486. Gould, E., Vail, N., Wagers, M., and Gross, C.G. (2001). Adult-generated hippocampal and neocortical neurons in macaques have a transient existence. Proceedings of the National Academy of Sciences of the United States of America 98, 10910-10917. Hayley, S., Poulter, M.O., Merali, Z., Anisman, H. (2005). The pathogenesis of clinical depression: Stressor- and cytokine-induced alterations of neuroplasticity. Elsevier 135, 659-678. Heo, J.C., Park, C.H., An, S.M., and Park, J.Y. (2007). Gastrodia elata Blume protects against stress-induced gastric mucosal lesions in mice. International Journal of Molecular Medicine 20, 209-215. Huang, N.K., Chern, Y., Fang, J.M., Lin, C.I., Chen, W.P., and Lin, Y.L. (2007). Neuroprotective principles from Gastrodia elata. J Nat Prod 70, 571-574. Hsieh, M.T., Wu, C.R., and Chen, C.F. (1997). Gastrodin and p-hydroxybenzyl alcohol facilitate memory consolidation and retrieval, but not acquisition, on the passive avoidance task in rats. Journal of Ethnopharmacology 56, 45-54. Hsieh, C.L., Lin, J.J., Chiang, S.Y., Su, S.Y., Tang, N.Y., Lin, G.G., Lin, I.H., Liu, C.H., Hsiang, C.Y., Chen, J.C., and Ho, T.Y. (2007). Gastrodia elata modulated activator protein 1 via c-Jun N-terminal kinase signaling pathway in kainic acid-induced epilepsy in rats. Journal of Ethnopharmacology 109, 241-247. Hwu, H.G., Yeh, E.K., and Chang, L.Y. (1989). Prevalence of psychiatric disorders in Taiwan defined by the Chinese Diagnostic Interview Schedule. Acta Psychiatr Scand 79, 136-147. Jama, A., Cecchi, M., Calvo, N., Watson, S.J., and Akil, H. (2008). Inter-individual differences in novelty-seeking behaviour in rats predict differential responses to desipramine in the forced swm test. Psychopharmacology (Berl.) 198, 333-340. Jiang, W., Gu, W., Brannstrom, T., Rosqvist, R., and Wester, P. (2001). Cortical neurogenesis in adult rats after transient middle cerebral artery occlusion. Stroke 32, 1201-1207. Jung, J.W., Yoon, B.H., Oh, H.R., Ahn, J.H., Kim, S.Y., Park, S.Y., and Ryu, J.H. (2006). Anxiolytic-like effects of Gastrodia elata and its phenolic constituents in mice. Biol Pharm Bull 29, 261-265. Jung, T.Y., Suh, S.I., Lee, H., Kim, I.S., Kim, H.J., Yoo, H.S., and Lee, S.R. (2007). Protective effects of several components of Gastrodia elata on lipid peroxidation in gerbil brain homogenates. Phytother Res 21, 960-964. Kellner, C.H., and Bourgon, L.N. (1998). Combining ECT and antidepressants: time to reassess. J ECT 14, 65-67. Kim, H.J., Lee, S.R., and Moon, K.D. (2003). Ether fraction of methanol extracts of Gastrodia elata, medicinal herb protects against neuronal cell damage after transient global ischemia in gerbils. Phytother Res 17, 909-912. Khawam, E.A., Laurencic, G., and Malone, D.A., Jr. (2006). Side effects of antidepressants: an overview. Cleve Clin J Med 73, 351-353, 356-361. Koketsu, D., Mikami, A., Miyamoto, Y., and Hisatsune, T. (2003). Nonrenewal of neurons in the cerebral neocortex of adult macaque monkeys. J Neurosci 23, 937-942. Kornack, D.R., and Rakic, P. (2001). Cell proliferation without neurogenesis in adult primate neocortex. Science 294, 2127-2130. Kuhn, H.G., Dickinson-Anson, H., and Gage, F.H. (1996). Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. The Journal of neuroscience : the official journal of the Society for Neuroscience 16, 2027-2033. Lee, J., Duan, W., Long, J.M., Ingram, D.K., and Mattson, M.P. (2000). Dietary restriction increases the number of newly generated neural cells, and induces BDNF expression, in the dentate gyrus of rats. Journal of molecular neuroscience : MN 15, 99-108. Lee, J.Y., Jang, Y.W., Kang, H.S., Moon, H., Sim, S.S., and Kim, C.J. (2006). Anti-inflammatory action of phenolic compounds from Gastrodia elata root. Arch Pharm Res 29, 849-858. Lichtenwalner, R.J., Forbes, M.E., Bennett, S.A., Lynch, C.D., Sonntag, W.E., and Riddle, D.R. (2001). Intracerebroventricular infusion of insulin-like growth factor-I ameliorates the age-related decline in hippocampal neurogenesis. Neuroscience 107, 603-613. Liu, J., and Mori, A. (1992). Antioxidant and free radical scavenging activities of Gastrodia elata Bl. and Uncaria rhynchophylla (Miq.) Jacks. Neuropharmacology 31, 1287-1298. Lopez-Rubalcava, C., and Lucki, I. (2000). Strain differences in the behavioural effects of antidepressant drugs in the rat forced swimming test. Neuropsychopharmacology 22, 191-199. Lucassen, P.J., Muller, M.B., Holsboer, F., Bauer, J., Holtrop, A., Wouda, J., Hoogendijk, W.J., De Kloet, E.R., and Swaab, D.F. (2001). Hippocampal apoptosis in major depression is a minor event and absent from subareas at risk for glucocorticoid overexposure. Am J Pathol 158, 453-468. Lucki, I. (1997). The forced swimming test as a model for core and component behavioral effects of antidepressant drugs. Behav. Pharmacol 8, 523-532. Madsen, T.M., Treschow, A., Bengzon, J., Bolwig, T.G., Lindvall, O., and Tingstrom, A. (2000). Increased neurogenesis in a model of electroconvulsive therapy. Biological psychiatry 47, 1043-1049. Magavi, S.S., Leavitt, B.R., and Macklis, J.D. (2000). Induction of neurogenesis in the neocortex of adult mice. Nature 405, 951-955. Malberg, J.E., Eisch, A.J., Nestler, E.J., and Duman, R.S. (2000). Chronic antidepressant treatment increases neurogenesis in adult rat hippocampus. The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 9104-9110. McClintock, S.M., Brandon, A.R., Husain, M.M., and Jarrett, R.B. (2011). A systematic review of the combined use of electroconvulsive therapy and psychotherapy for depression. J ECT 27, 236-243. Miller, L.J. (1994). Use of electroconvulsive therapy during pregnancy. Hosp Community Psychiatry 45, 444-450. Nierenberg, A.A. (1992). The medical consequences of the selection of an antidepressant. J Clin Psychiatry 53 Suppl, 19-24. Niu, Q., Niu, P., and He, S. (2004). Effect of gastrodia elata on learning and memory impairment induced by aluminum in rats. Wei Sheng Yan Jiu 33, 45-48. Nobler, M.S., and Sackeim, H.A. (2008). Neurobiological correlates of the cognitive side effects of electroconvulsive therapy. J ECT 24, 40-45. Parent, J.M., Vexler, Z.S., Gong, C., Derugin, N., and Ferriero, D.M. (2002). Rat forebrain neurogenesis and striatal neuron replacement after focal stroke. Ann Neurol 52, 802-813. Page, M.E.; Brown, K., and Lucki, I. (2003). Simultaneous analyses of the neurochemical and behavioural effects of the norepinephrine reuptake inhibitor reboxetine in a rat model of antidepressant action. Psychopharmacology (Berl.) 165, 194-201. Page, M.E.; Detke, M.J.; Dalvi, A.; Kirby, L.G., and Lucki, I. (1999). Serotonergic mediation of the effects of fluoxetine, but not desipramine, in the rat forced swimming test. Psychopharmacology (Berl.) 147, 162-167. Pittenger, C., and Duman, R.S. (2008). Stress, depression, and neuroplasticity: a convergence of mechanisms. Neuropsychopharmacology 33, 88-109. Porsolt, R.D., Anton, G., Blavet, N., and Jalfre, M. (1978a). Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol 47, 379-391. Porsolt, R.D., Bertin, A., and Jalfre, M. (1978b). 'Behavioural despair' in rats and mice: strain differences and the effects of imipramine. Eur J Pharmacol 51, 291-294. Porsolt, R.D., Le Pichon, M., and Jalfre, M. (1977). Depression: a new animal model sensitive to antidepressant treatments. Nature 266, 730-732. Potter, W.Z., and Rudorfer, M.V. (1993). Electroconvulsive therapy--a modern medical procedure. N Engl J Med 328, 882-883. Qu, T., Brannen, C.L., and Kim, H.M. (2001). Human neural stem cells improve cognitive function of aged brain. Neuroreport 12, 1127-1132. Quackenbush, J. (2002). Microarray data normalization and transformation. Nature Genetics 32, 496-501. Quartermain, D., deSoria, V.G., and Kwan, A. (2001). Calcium channel antagonists enhance retention of passive avoidance and maze learning in mice. Neurobiology of learning and memory 75, 77-90. Quartermain, D., and Garcia deSoria, V. (2001). The effects of calcium channel antagonists on short- and long-term retention in mice using spontaneous alternation behavior. Neurobiology of learning and memory 76, 117-124. Richelson, E. (2003). Interactions of antidepressants with neurotransmitter transporters and receptors and their clinical relevance. J Clin Psychiatry 64 Suppl 13, 5-12. Sambunaris, A., Hesselink, J.K., Pinder, R., Panagides, J., and Stahl, S.M. (1997). Development of new antidepressants. J Clin Psychiatry 58 Suppl 6, 40-53. Scott, B.W., Wojtowicz, J.M., and Burnham, W.M. (2000). Neurogenesis in the dentate gyrus of the rat following electroconvulsive shock seizures. Experimental neurology 165, 231-236. Sheline, Y.I., Wang, P.W., Gado, M.H., Csernansky, J.G., Vannier, M.W. (1996). Hippocampal atrophy in recurrent major depression. Proceedings of the National Academy of Sciences of the United States of America 93, 3908-3913. Shors, T.J., Miesegaes, G., Beylin, A., Zhao, M., Rydel, T., and Gould, E. (2001). Neurogenesis in the adult is involved in the formation of trace memories. Nature 410, 372-376. Smyth, G.K., and Speed, T. (2003). Normalization of cDNA microarray data. Elsevier 31, 265-273. Stunden, C.E., Filosa, J.A., Garcia, A.J., Dean, J.B., and Putnam, R.W. (2001). Development of in vivo ventilatory and single chemosensitive neuron responses to hypercapnia in rats. Respir Physiol 127, 135-155. Sun, X.F., Wang, W., Wang, D.Q., and Du, G.Y. (2004). Research progress of neuroprotective mechanisms of Gastrodia elata and its preparation. Zhongguo Zhong Yao Za Zhi 29, 292-295. Terra, J.L.,( 2008). Suicide risk and depression. Rev Prat. 58, 385-388. Thase, M.E., 2005. Mood disorders: neurobiology. In: B.J. Sadock and V.A. Sadock (Eds.), Comprehensive Textbook of Psychiatry, Lippincott Williams & Wilkins, Philadelphia, pp.1594-1603. Van Praag, H., Christie, B.R., Sejnowski, T.J., and Gage, F.H. (1999a). Running enhances neurogenesis, learning, and long-term potentiation in mice. Proceedings of the National Academy of Sciences of the United States of America 96, 13427-13431. Van Praag, H., Kempermann, G., and Gage, F.H. (1999b). Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus. Nature neuroscience 2, 266-270. Walker, R., and Swartz, C.M. (1994). Electroconvulsive therapy during high-risk pregnancy. Gen Hosp Psychiatry 16, 348-353. World Health Organization. The global burden of disease: 2004 update. WHO: Geneva, 2008. Yang, Y., Han, F.M., Du, P., and Chen, Y. (2010). Pharmacokinetics of gastrodin from compound Tianma granule in rats. Acta pharmaceutica Sinica 45, 484-488. Zhang, B., Kirov, S., Snoddy, J. (2005). WebGestalt: an integrated system for exploring gene sets in various biological contexts. (2005). Nucleic Acids Research 33, 741-748. Zhong, P., Liu, W., Gu, Z., and Yan, Z. (2008). Serotonin facilitates long-term depression induction in prefrontal cortex via p38 MAPK/Rab5-mediated enhancement of AMPA receptor internalization. J Physiol 586, 4465-4479. Zhou, L., Del Villar, K., Dong, Z., and Miller, C.A. (2004). Neurogenesis response to hypoxia-induced cell death: map kinase signal transduction mechanisms. Brain Res 1021, 8-19. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6900 | - |
dc.description.abstract | 憂鬱症是精神疾病中盛行率極高的情感性疾病,並且根據 WHO 公佈的統計顯示, 全球憂鬱症患者逐年攀升,並且將會在2020年成為精神疾病之首。由於目前抗憂鬱劑治療方式會帶給許多病人嚴重的副作用,例如焦慮,腸胃問題,以及性功能衰減。因此如何預防憂鬱症是個重要的課題。本研究應用中藥食療預防醫學探討天麻水萃物對大鼠情感相關腦組織之影響,如前額葉皮質,海馬迴,以及紋狀體。本研究以管餵的方式將0.5 g/kg BW劑量之天麻水萃物 (WGE) 給予 Sprangue-Dawley大鼠,每日一次並為期連續21天,並進一步利用強迫游泳試驗(FST) 來誘導憂鬱症。研究結果顯示,天麻水萃物試驗組 (WGE) 的大鼠在FST 中比控制組 (NE) 的老鼠取得有顯著性差異較低的不活動時間,顯示 FST有成功的誘導大鼠憂鬱症行為。在 21天試驗期結束後,將大鼠犧牲,並取得前額葉皮質,海馬迴,紋狀體等憂鬱症相關之大腦區域組織,接著萃取total mRNA. 進一步利用微陣列分析來探討可能的天麻抗憂鬱機制,並運用即時聚合酶鏈式反應來驗證實驗結果。微陣列分析結果顯示在大鼠前額葉皮質以及海馬迴中,axongenesis, neurogenesis, nervous system development, 以及多巴胺分泌 (dopamine secretion) 為最有可能的機制,根據與資料庫的資料比對後取得的p-value值大小排列,最有可能的機制為最小的數值。然而紋狀體的微陣列分析結果並沒有顯式與憂鬱症有關的機制。即時聚合酶鏈式反應實驗結果驗證了WGE確實有顯著性的提升與神經可塑性 (neuroplasticity) 相關的基因表現量, 如Map1b,RhoA, profilin-1, 以及CRMP2 (p < 0.05).因此,神經可塑性為天麻水萃物之可能的抗憂鬱機制。本研究結果可為未來同一試驗模式但不同體系之研究做為參考,並為未來抗憂鬱製藥的研究有所貢獻,尤其是中藥天麻在基因體學中之抗憂鬱研究領域。 | zh_TW |
dc.description.abstract | Depression has been a serious issue, as the annual worldwide reported cases of depression have been constantly increasing, according to the statistics published by WHO. In fact, WHO predicts that it will be the leading psychological disease by year 2020. However, antidepressants generally demonstrate serious side-effects, such as anxiety disorders, gastrointestinal problems, and sexual dysfunction in patients; therefore, it is important to find an effective way to prevent the occurrence of depression. In this research, Gastrodia eleta Bl., an Oriental herb that has been shown to demonstrate anti-depression effects, was investigated in the form of water extraction. In the present study, the water extract of Gastrodia eleta Bl. (WGE) was orally administered to Sprague-Dawley rats at the dose of 0.5 g/kg BW each day for 21 consecutive days. Forced swimming test (FST) was performed to induce depression in the rodent model, and results showed that the WGE group demonstrated shorter immobile time compared to the negative control group, indicating that FST has successfully induced depression-like behaviours in these rats. Total mRNA samples were then obtained from the frontal cortex, hippocampus, and striatum, after having sacrificed the rats after 21 days. In order to deduce a possible anti-depression pathway at the genomic level, cDNA microarray was performed to generate gene expression profiles of depression relevant brain regions: cortex, hippocampus, and striatum. To confirm the findings, real-time polymerase chain reaction (QRT-PCR) analysis of several neuroplasticity-related, differentially expressed genes, was performed. The microarray data showed that WGE altered axongenesis/neurogenesis, nervous system development, and dopamine secretion pathways in cortex and hippocampus, from the evidence that they yield the lowest p-values from all other pathway matches with the KEGG database. However, there were no depression-related pathways shown in the results of the microarray data for the striatum. QRT-PCR results validated that genes involved in neurogenesis, such as Map1b, RhoA, profilin-1, and CRMP2 were significantly altered (p < 0.05) in the cortex and hippocampus. Therefore, neuroplasticity might be the mechanism that WGE takes. The neuroplasticity effects of WGE in the rodent model of antidepressant action strengthen the case for further testing the results in the same context using other tools, and can serve as the basis of future antidepressant drugs, especially in the area of WGE demonstrating anti-depressant effects in the aspect of genomics. | en |
dc.description.provenance | Made available in DSpace on 2021-05-17T09:20:42Z (GMT). No. of bitstreams: 1 ntu-101-R98641039-1.pdf: 1068180 bytes, checksum: d583f97c4a86e971c7aafe9585082182 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | Table of Contents
ABBREVIATIONS…………………………………………………………………...I ABSTRACT (CHINESE)…………………………………………………………..…II ABSTRACT (ENGLISH)……………………………………………….……………IV TABLE OF CONTENTS………………………………………………………….….V LIST OF TABLES…………………………………………………………………... VI LIST OF FIGURES……………………….………………………………………….VII. Chapter 1: INTRODUCTION…………………………………………………………1 Chapter 2: LITERATURE REVIEW………………………………………………… 3 Chapter 3: PURPOSE OF STUDY………………………………………………….. 17 Chapter 4: EXPERIMENTAL FRAMEWORK……………………………………... 18 Chapter 5: MATERIALS AND METHODS………………………………………… 19 Section 1: Experimental Materials……………………………………………... 19 Section 2: Experimental Methods……………………………………………….20 5.2.1: Sample preparation and dosage………………………………………….20 5.2.2: Animals and treatments……………………………………………….…20 5.2.3: Weight change and food intake………………………………………….21 5.2.4: Forced swimming test…………………………………………………...21 5.2.5: Tissue dissection………………………………………………………...22 5.2.6: RNA extraction, purification, and quality control………………………22 5.2.7: Microarray……………………………………………………………....23 5.2.8: Microarray data analysis………………………………………………..25 5.2.9: QPCR…………………………………………………………………...27 5.2.10: Statistical analysis…………………………………………………… .30 Chapter 6: RESULTS…………………………………………………………………..31 Chapter 7: DISCUSSION……………………………………………………………....73 Section 1: Effect of Gastrodia elata Bl. on weight change and food intake.....73 Section 2: Effect of Gastrodia elata Bl. on rodents’ performance in FST………74 Section 3: Advantages and disadvantages of microarray analysis………………76 Section 4: Microarray analysis on the potential mechanism in the cortex………77 Section 5: Microarray analysis on the potential mechanism in hippocampus…...78 Section 6: Microarray analysis on the potential mechanism in the striatum…….78 Section 7: Neurogenesis, neuroplasticty, neurotransmitters, antidepressants, and depression………………………………………………………………79 Section 8: Overall discussion……………………………………………………84 Chapter 8: CONCLUSION…………………………….................................................85 Chapter 9: REFERENCES…………………………………………………….............86 List of Tables Table 1. Summary of side-effects of different classes of antidepressants…………8 Table 2. List of primers…………………………………………………………….27 Table 3. Gene list of significant genes regulated by WGE in the frontal cortex…..40 Table 4. Table of possible KEGG pathways of genes regulated by WGE in the frontal cortex……………………………………………………………………..45 Table 5. Gene list of significant genes regulated by WGE in the hippocampus…..49 Table 6. Table of possible KEGG pathways of genes regulated by WGE in the hippocampus………………………………………………………….…..60 Table 7. Gene list of significant genes regulated by WGE in the striatum………...65 Table 8. Table of possible KEGG pathways of genes regulated by WGE in the striatum…………………………………………………………………...69 List of Figures Figure 1. Image of dentritic structure under stress….….……………………………...11 Figure2. Genomics of antidepressants and neuroplasticity……………………………12 Figure 3. Genomics of antidepressants, neuroplasticity, and neurotransmitters…..…..13 Figure 4. Genomics of neuroplasticity and environmental stimulators…......................14 Figure 5. Overview of pathology of depression……………………………………….15 Figure 6. Effect of WGE on weight change of rats……………………………………33 Figure 7. Effect of WGE on food intake of rats……………………………………….34 Figure 8. Effect of WGE on FST performance………………………………………..35 Figure 9. Normalized data of microarray analysis…………………………………….36 Figure 10. Principal component analysis of normalized microarray data……………..37 Figure 11. Venn diagram of significant genes in cortex……………………………….38 Figure 12. Heatmap of significant genes in cortex…………………………………….44 Figure 13 Network analysis of significant genes in cortex……………………………47 Figure 14. Venn diagram of significant genes in hippocampus……………………….48 Figure 15. Heatmap of significant genes in hippocampus…………………………….58 Figure 16. Network analysis of significant genes in hippocampus……………………62 Figure 17. Venn diagram of significant genes in striatum……………………………..64 Figure 18. QPCR results of neurogenesis-related genes in cortex…………………….71 Figure 19. QPCR results of neurogenesis-related genes in hippocampus……………..72 | |
dc.language.iso | en | |
dc.title | 以基因體層面探討天麻水萃物之抗憂鬱功效 | zh_TW |
dc.title | Transcriptomic Profile of the Anti-Depressant Effects of Gastrodia elata Bl. | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡孟勳(Motion Tsai),賴文崧(Wen-Sung Lai),蘇冠賓(Cobol Su) | |
dc.subject.keyword | 憂鬱症,強迫游泳試驗,天麻水萃物,微陣列分析,神經可塑性, | zh_TW |
dc.subject.keyword | depression,forced swimming test,Gastrodia elata Bl.,microarray analysis,neuroplasticity, | en |
dc.relation.page | 97 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2012-02-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-101-1.pdf | 1.04 MB | Adobe PDF | 檢視/開啟 |
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