請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68925
標題: | "α2, 8連結之雙唾液酸結構在體液免疫中的角色以及與唾液酸結合之似免疫球蛋白凝集素E型的相互作用" The role of α2, 8-diSialyl motif in humoral immunity and its interaction with Siglec-E |
作者: | Ho-Yang Tsai 蔡和仰 |
指導教授: | 林國儀(Kuo-I Lin) |
關鍵字: | α2,8連結之雙唾液酸結構,唾液酸結合之似免疫球蛋白凝集素E型,第六型α-2,8唾液酸轉移?,體液免疫,B1細胞, α2, 8-diSialyl motif,Siglec-E,ST8sia VI,B1 B cell,humoral immunity, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | The role of increasing α2, 8-diSialyl motif synthesized by ST8Sia VI on differentiating B cell surface was unclear. Additionally, it is reported that there is only Siglec-E that can recognize α2, 8-diSialyl motif. We have previously established B cell specific ST8Sia VI knockout (cKO) mice. Our preliminary results showed that ST8Sia VI cKO mice produced higher levels of IgM after immunization. Therefore, in this thesis, we focused on which subsets of B cells contributed to function of α2, 8-diSialyl motif and whether the interplay of Siglec-E was involved.
First, we generated that Rbpj and ST8Sia VI B cell-specific double knockout mice, which featured marginal zone B (mzB) cells and α2, 8-diSialyl motif deficiency. These mice showed higher IgM production at the levels similar to those produced by ST8Sia VI cKO mice upon TI antigen immunization. Furthermore, sorted follicle B (foB) cells and B1 cells, but not mzB cells, from ST8Sia VI cKO mice showed stronger activation after stimulation with either LPS or anti-IgM. We also generated SS-KO mice, whose ST8Sia VI and Siglece were deleted, to determine the interaction of Siglec-E and α2, 8-diSialyl motif. We found that SS-KO mice still produced higher amounts of antigen specific IgM than wild type (WT) mice did upon NP-Ficoll immunization, but that the levels of antigen specific IgM in SS-KO mice were similar to that in WT mice after NP-LPS immunization. These results suggested that the interaction between α2, 8-diSialyl motif and Siglec-E may be involved in TLR4 signaling. Furthermore, we found that the higher activation of foB cells from ST8Sia VI cKO mice were compromised after co-cultured with macrophages or neutrophils form Siglece KO mice after LPS stimulation. On the other hand, both Siglece KO and ST8Sia VI cKO B1 B cells were activated better after LPS stimulation. In conclusion, α2, 8-diSialyl motif not only plays an inhibitory role on foB cells but also acts through Siglec-E in both cis- and trans- manners on B1 and foB cells separately. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68925 |
DOI: | 10.6342/NTU201703213 |
全文授權: | 有償授權 |
顯示於系所單位: | 免疫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 1.83 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。