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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林國儀(Kuo-I Lin) | |
dc.contributor.author | Ho-Yang Tsai | en |
dc.contributor.author | 蔡和仰 | zh_TW |
dc.date.accessioned | 2021-06-17T02:42:26Z | - |
dc.date.available | 2022-09-13 | |
dc.date.copyright | 2017-09-13 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-16 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68925 | - |
dc.description.abstract | The role of increasing α2, 8-diSialyl motif synthesized by ST8Sia VI on differentiating B cell surface was unclear. Additionally, it is reported that there is only Siglec-E that can recognize α2, 8-diSialyl motif. We have previously established B cell specific ST8Sia VI knockout (cKO) mice. Our preliminary results showed that ST8Sia VI cKO mice produced higher levels of IgM after immunization. Therefore, in this thesis, we focused on which subsets of B cells contributed to function of α2, 8-diSialyl motif and whether the interplay of Siglec-E was involved.
First, we generated that Rbpj and ST8Sia VI B cell-specific double knockout mice, which featured marginal zone B (mzB) cells and α2, 8-diSialyl motif deficiency. These mice showed higher IgM production at the levels similar to those produced by ST8Sia VI cKO mice upon TI antigen immunization. Furthermore, sorted follicle B (foB) cells and B1 cells, but not mzB cells, from ST8Sia VI cKO mice showed stronger activation after stimulation with either LPS or anti-IgM. We also generated SS-KO mice, whose ST8Sia VI and Siglece were deleted, to determine the interaction of Siglec-E and α2, 8-diSialyl motif. We found that SS-KO mice still produced higher amounts of antigen specific IgM than wild type (WT) mice did upon NP-Ficoll immunization, but that the levels of antigen specific IgM in SS-KO mice were similar to that in WT mice after NP-LPS immunization. These results suggested that the interaction between α2, 8-diSialyl motif and Siglec-E may be involved in TLR4 signaling. Furthermore, we found that the higher activation of foB cells from ST8Sia VI cKO mice were compromised after co-cultured with macrophages or neutrophils form Siglece KO mice after LPS stimulation. On the other hand, both Siglece KO and ST8Sia VI cKO B1 B cells were activated better after LPS stimulation. In conclusion, α2, 8-diSialyl motif not only plays an inhibitory role on foB cells but also acts through Siglec-E in both cis- and trans- manners on B1 and foB cells separately. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T02:42:26Z (GMT). No. of bitstreams: 1 ntu-106-R04449007-1.pdf: 1874268 bytes, checksum: d86fca25f5fbba925cecacf2c2003f96 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 致謝 i
中文摘要 ii Abstract iii List of Figures vii Chapter 1 Introduction 1 1.1 B cell development and subsets 1 1.2 Innate humoral immunity and B1 B cells 3 1.3 Glycosylation and α2, 8-diSialyl motif 4 1.4 Siglec-E in immune system 6 Rationales and specific aims 8 Chapter 2 Materials and Methods 10 2.1 Mice 10 2.2 Cell culture 11 2.3 Flow cytometry 12 2.4 ELISA 14 2.5 RNA isolation and RT-qPCR 14 Chapter 3 Results 16 3.1 Follicle B cells contribute more anti-IgM production in late phase of immune response to TI-antigen. 16 3.2 B1 B cells increase in Siglece-/- mice and activate better. 17 3.3 SS-KO mice show normal B cell development. 18 3.4 SS-KO mice respond to NP-Ficoll immunization more vigorously than WT mice but not to NP-LPS immunization. 19 3.5 Total splenic B cells from Siglec-E, ST8Sia VI cKO and dKO mice activated similarly 20 3.6 B1 B cells adopted cis-interaction between α2, 8-diSialyl motif and Siglec-E. 21 Chapter 4 Discussion 23 Chapter 5 References 28 Chapter 6 Figures 36 | |
dc.language.iso | zh-TW | |
dc.title | "α2, 8連結之雙唾液酸結構在體液免疫中的角色以及與唾液酸結合之似免疫球蛋白凝集素E型的相互作用" | zh_TW |
dc.title | The role of α2, 8-diSialyl motif in humoral immunity and its interaction with Siglec-E | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李建國(Chien-Kuo LEE),安形高志(Takashi Angata) | |
dc.subject.keyword | α2,8連結之雙唾液酸結構,唾液酸結合之似免疫球蛋白凝集素E型,第六型α-2,8唾液酸轉移?,體液免疫,B1細胞, | zh_TW |
dc.subject.keyword | α2, 8-diSialyl motif,Siglec-E,ST8sia VI,B1 B cell,humoral immunity, | en |
dc.relation.page | 53 | |
dc.identifier.doi | 10.6342/NTU201703213 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-08-16 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 免疫學研究所 | zh_TW |
顯示於系所單位: | 免疫學研究所 |
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