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標題: | 犬貓血清中非運鐵蛋白結合鐵的定量和腎臟病惡化的相關性 Quantification of non-transferrin bound iron in serum and its association to the progression of canine and feline kidney disease |
作者: | Po-Yao Huang 黃柏堯 |
指導教授: | 李雅珍(Ya-Jane Lee) |
關鍵字: | 血清非運鐵蛋白結合鐵,游離鐵,犬腎臟疾病,貓腎臟疾病, Non-transferrin-bound iron,catalytic iron,labile iron,canine kidney disease,feline kidney disease, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 研究背景 在人類臨床與實驗動物研究中,發現當腎臟疾病造成體內鐵質代謝失去平衡時,觀察到血清中非運鐵蛋白結合鐵(Non-transferrin-bound iron, NTBI)濃度會上昇。 研究目的 我們假設在有腎臟疾病的犬貓病患的血清中,非運鐵蛋白結合鐵濃度相較於健康組會有上昇的現象。血清非運鐵蛋白結合鐵濃度在急性腎損傷或慢性腎衰竭的犬貓中,可能具有成為新興的預後指標的潛力。 研究對象 39隻健康的犬隻, 19隻患有慢性腎衰竭的犬隻,5隻患有急性腎損傷的犬隻;11隻健康的貓,29隻患有慢性腎衰竭的貓,2隻患有急性腎損傷的貓。 實驗方法 將血清經過超過濾去除運鐵蛋白以及其他大分子蛋白後,再使用感應耦合電漿質譜儀(inductively coupled plasma mass spectrometry, ICP-MS)測量其中的血清非運鐵蛋白結合鐵,實驗中同時偵測兩種鐵同位素: 鐵56以及鐵57 實驗結果 血清非運鐵蛋白結合鐵在犬貓的各組中(控制組,慢性腎臟病組,急性腎衰竭組)並無法發現顯著差異。在患有腎臟病的犬隻中,惡化組與無惡化組的血清非運鐵蛋白結合鐵具有差異(血清非運鐵蛋白結合鐵56: Median 319.3 ng/ml vs. 211.3 ng/ml; 血清非運鐵蛋白結合鐵57: Median 682.9 ng/ml vs. 528.5 ng/ml)。血清非運鐵蛋白結合鐵-56在犬隻中與較快的呼吸速率、較多的分葉中性球、較少的嗜酸性球、較低的平均紅血球血紅素、較低的網狀紅血球血紅蛋白當量(reticulocyte hemoglobin equivalent),以及較低的血清游離鈣具有顯著相關性;清非運鐵蛋白結合鐵-57在犬隻中則與較多的分節中性球以及較低的嗜酸性球有顯著相關性。血清非運鐵蛋白結合鐵-56/57在貓中皆與較多的血小板以及較低的體溫顯著相關。 實驗結果與重要發現 血清非運鐵蛋白結合鐵56以及57在患有腎臟病的犬隻具有做為預後指標的潛力。而犬隻與貓血清非運鐵蛋白在腎臟病所扮演的角色以及代謝的途徑可能不同。 Background Non-transferrin-bound iron (NTBI) increases after the disrupted homeostasis of iron was reported in humans and laboratory animals with renal diseases. Objectives We hypothesized that serum NTBI concentration could increase in canine and feline with kidney diseases. It can be a novel prognostic factor of chronic kidney disease and acute kidney injury in small animal medicine. Samples Thirty-nine healthy dogs, 19 dogs with CKD, five dogs with AKI, 11 healthy cats, 29 cats with CKD, two cats with AKI were enrolled. Methods The serum non-transferrin-bound iron concentration was measured using simple ultrafiltration inductively coupled plasma mass spectrometry. Both 56Fe 57Fe (termed as NTBI-56, NTBI-57) were monitored. Results No significant difference was found among groups in dogs and cats (control, AKI, CKD). There was a difference of NTBI-56/57 between dogs with progression and without progression (NTBI-56: Median 319.3 ng/ml vs. 211.3 ng/ml; NTBI-57: Median 682.9 ng/ml vs. 528.5 ng/ml). In dogs, NTBI-56 was significantly correlated with high respiratory rate and segmented cell count, and with low eosinophil count, MCH, reticulocyte hemoglobin equivalent, and serum ionized calcium; NTBI-57 was significantly correlated with high segmented cell count and with low eosinophil count. In cats, both NTBI-56/57 were significantly correlated with high platelet count and with low body temperature.. Conclusions and Clinical Importance NTBI-56/57 may be a prognostic markers in dogs with kidney diseases, and the roles of NTBI may be different between in canine and feline. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68827 |
DOI: | 10.6342/NTU202003668 |
全文授權: | 有償授權 |
顯示於系所單位: | 臨床動物醫學研究所 |
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