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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68467
標題: | 玻尿酸接枝阿黴素及近紅外光染料作為CD44標靶之奈米載體以結合光熱與化學治療 CD44 targeted delivery of hyaluronic acid-Doxorubicin/NIR dye conjugates as nanocarrier for the photothermal/chemotherapy |
作者: | Wan-Yun Lien 連婉蘊 |
指導教授: | 謝銘鈞(Ming-Jium Shieh) |
關鍵字: | 阿黴素,光熱治療,玻尿酸,CD44,奈米顆粒, Doxorubicin,Photothermal Therapy,Hyaluronic Acid,CD44,Nanoparticle, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 本作將光感藥物IR-780與化療藥物阿黴素(doxorubicin)分別接枝在親水的天然高分子玻尿酸上,形成親疏水兩性的高分子:HA-IR與HA-DOX。藉由親疏水的特性,HA-IR與HA-DOX可形成奈米等級的顆粒,本作中稱為HA-IR/DOX NP。根據不同的HA-IR與HA-DOX比例,HA-IR/DOX NP平均粒徑介於120到170奈米不等,其粒徑具有相對狹窄的分佈。由穩定性測試,此奈米顆粒在保存環境下具有一定的穩定性。由細胞測試,HA-IR/DOX NP可以標靶到CD44過度表現的乳癌細胞MDA-MB-231。而光熱測試則可見HA-IR/DOX NP仍具有光熱升溫的效果。此外HA-IR的螢光,可進行小鼠體內造影。由造影結果顯示HA-IR/DOX NP在尾靜脈注射後會累積至腫瘤。由小鼠抗腫瘤試驗,HA-IR/DOX NP在近紅外光雷射照射下,可達到良好的抗腫瘤效果。 In this study, a photothermal drug, IR-780, and a chemotherapy drug, doxorubicin, were conjugated to hyaluronic acid respectively to form two kinds of amphiphilic polymers, HA-IR and HA-DOX. Based on the amphiphilic property, the HA-IR and HA-DOX mixture can self-assembly to nanoparticles, HA-IR/DOX NP. With different HA-IR/HA-DOX ratios, HA-IR/DOX NPs exhibited particle size from 120 to 170 nm with relatively narrow size distribution. The stability test showed that the HA-IR/DOX NP were stable in 4°C storage condition. From in vitro test, HA-IR/DOX NP targeted to CD44-overexpression cancer cell line, MDA-MB-231. The HA-IR/DOX NP showed photothermal effect and phototoxicity both in vitro and in vivo. On the other hand, the fluorescent property of IR-780 provide a function of in vivo imaging, which shows that the HA-IR/DOX NP accumulated to the tumor via EPR effect and CD44-targeting ligand. The antitumor effect showed that the HA-IR/DOX NP combined the chemo-therapy and the photo-thermal therapy. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68467 |
DOI: | 10.6342/NTU201703079 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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