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標題: | 一種新型光敏劑(四氨基鋅酞菁)的特性分析與其損害癌細胞之效果 Characteristics of a New Photosensitizer (tetramino-zinc-phthalocyanine) and Its Cancer Cell Damage Effects |
作者: | Jian-He Yu 余建和 余建和 |
指導教授: | 楊志忠 |
關鍵字: | 光動力療法,四氨基鋅?菁, Photodynamic Therapy,tetramino-zinc-phthalocyanine, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 在本論文中,透過羧基以及胺基的脫水縮合反應,我們成功合成一種用共價鍵連結金奈米粒子的新型光敏劑(四氨基鋅酞菁),能夠有效的降低光敏劑從金奈米顆粒脫離的機率。在合成四氨基鋅酞菁完畢後,我們先測試了基本的光敏劑特性,包括吸收頻譜、產生單態氧能力、細胞暗毒性和細胞損害等,接著選用目前廣泛使用的四磺酸鋁酞菁來做為比較對象,我們將光敏劑接在金奈米棒的樣品泡在水中不同時間後,再利用穿透頻譜和細胞損害實驗來比較光敏劑脫落情形。我們發現四氨基鋅酞菁比起四磺酸鋁酞菁,光敏劑較少脫離,四氨基鋅酞菁只有在泡水十分鐘和泡水三十分鐘間有明顯的差距,然而四磺酸鋁酞菁卻隨著泡水時間增長,細胞損害效果隨之降低。這結果證明了比起用凡德瓦力鍵結的四磺酸鋁酞菁,用共價鍵鍵結的四氨基鋅酞菁能夠更穩定地將光敏劑接在金奈米棒上。 A new type of Pc-series photosensitizer, tetramino-zinc-phthalocyanine (ZnPcN), which can link with alpha-Thio-omega-carboxy poly(ethylene glycol) (HS-PEG-COOH) through covalent bonding, such that its linkage with an Au nanoparticle (NP) becomes stronger for reducing the escape rate is synthesized. After demonstrating its fundamental photosensitizer characteristics, its escape behavior is compared with that of the commonly used PcS-series photosensitizer, tetrasulfonate-aluminum-phthalocyanine (AlPcS), through the measurements of the decreasing trends of absorption and cell damage efficiency with increasing waiting time. It is found that the escape of ZnPcN is indeed slower than that of AlPcS. For ZnPcN, the cell damage efficiency decreases most significantly in the first 10 min of waiting and saturates 30 min after incubation. However, for AlPcS, the cell damage efficiency keeps decreasing with waiting time up to 4 hours after incubation. The stronger chemical bonding between ZnPcN and HS-PEG-COOH leads to less ZnPcN escape and hence a longer waiting time of high cell damage capability, when compared with the electro-static bonding between AlPcS and HS-PEG-NH2. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67617 |
DOI: | 10.6342/NTU201702142 |
全文授權: | 有償授權 |
顯示於系所單位: | 光電工程學研究所 |
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