含氟貼片和高露潔牙膏對牙釉質去礦化的影響及其表面結構分析

Abstract

實驗目的：為了達到齲齒預防或早期治療之目標，本研究以自行開發之含氟貼片，和市售含氟牙膏一同進行對牙釉質表面產生之影響分析，以確立含氟貼片具有安全性、便利性及預防齲齒功能。 實驗方法：將含氟貼片貼附後的牙釉質樣本進行 pH Cycling 實驗，市售牙膏為對照組，使用離子層析儀(IC)分析鈣離子流失和吸收量。並藉由X射線電子能譜儀(XPS)和掃瞄式電子顯微鏡(SEM)分析不同貼附時間對樣本表面結構之影響。 結果：SEM 和 XPS的表面分析和縱深測試結果顯示含氟貼片貼附24小時後，牙釉質表面主要沉積物為氟化鈣和氟磷灰石的混和物，同樣氟濃度的牙膏無法達到此效果，表面僅可能有氟磷灰石和氫氧化鈣存在。離子層析儀分析的結果顯示同樣貼附時間下，氟膠比市售牙膏更能減緩牙釉質中礦物質的流失程度，也能增加牙釉質的再礦化能力。 結論：由實驗結果推斷含氟貼片貼附後能在牙釉質表面形成氟化鈣和氟磷灰石，比牙膏更有效發揮氟化物的效果，不論在居家或臨床使用上，皆有相當潛力成為良好的齲齒預防方法。

Purpose：In order to prevent from dental caries or treat it in early lesion stage, we developed the fluoride releasing strip. To determine the fluoride releasing strip with security, convenience and function to prevent tooth decay, we compared the influence of fluoride strip and commercial toothpaste on enamel surface. Method：This experiment used pH Cycling system to test the specimens after fluoride strip attached, and commercial toothpaste as a control group. We used ion chromatography(IC) to analyze the loss and uptake of calcium. X-ray electron spectroscopy (XPS) and scanning electron microscopy (SEM) were used to analyze the effect of the different adhesion time on the enamel surface structure. Result：XPS and SEM results showed that fluoride strip attached to 24 hours, the main product of the enamel surface is mixture of calcium fluoride and fluorapatite. The same concentration of fluoride in the toothpaste cannot achieve this effect, the surface is only likely presence of fluorapatite and calcium hydroxide. Ion chromatography analysis revealed that the comparative fluoride strip with toothpaste, the fluoride strip to better slow down the degree of enamel mineral loss, and can increase the ability to enamel remineralization. Conclusion：The experimental results infer that the formation of calcium fluoride and fluorapatite in the enamel surface after use of fluoride strip. Contrast with toothpaste, the fluoride strip is more effective to show the anti-caries effects of fluoride. Therefore, in the home or clinical use, the strip has considerable potential to become a good prevention method of dental caries.