動物收容所犬隻及工作人員分離之金黃色葡萄球菌外毒素基因及抗藥性分析

Abstract

本研究針對收容所進行犬隻及工作人員的外鼻腔採樣，經分離出金黃色葡萄球菌 (Staphylococcus aureus; SA) 後，以聚合

The aims are to investigate the prevalence of staphylococcal enterotoxins and methicillin resistant Staphylococcus aureus (MRSA) in the nostrils of dogs and workers at Taoyuan Animal Protection Educative Garden. After S. aureus (SA) were identified, polymerase chain reaction (PCR) and reverse passive latex agglutination (RPLA) were used to detect genotype and phenotype of enterotoxins, respectively. Additionally, SCCmec, multilocus sequence typing (MLST), and exotoxin genes (eta, etb and tst) were screened for MRSA isolates and their relationships were further investigated by multiple-locus variable-number tandem repeat fingerprinting (MLVF). The isolation rates of SA and MRSA for shelter dogs were 49.0% (216/441) and 1.6% (7/441), and for workers were 44.4% (4/9) and 33.3% (3/9), respectively. The isolation rates of SA in summer (61.9%) and in adult dogs (59.2%) were significantly higher than those in winter (35.8%) and in juveniles (33.3%) (P < 0.001). The detection rate of SA with enterotoxn gene (se) in winter was 70.7%. The most frequent enterotoxin genotypes and combination profiles of the SA isolates were (sea, seb, seg, sei, sem, sen, seo, seu) and (sea, sea-seb and seg-sei-sem-sen-seo-seu), respectively. The (sea-seb) was found only in winter and (seg-sei-sem-sen-seo-seu) appeared a higher frequency in summer than in winter. MRSA isolates were identified as SCCmec IV (60.0%) and VII (40.0%), and they shared se combination profiles of (sec-seg-sei-sel-sem-sen-seo-seu) and (seb-sek-seq related). se between MRSA and MSSA were with few overlappins. Antimicrobial testing showed that the SA isolates were resistant to tetracycline (22.6%) and were sensitive to trimethoprim-sulfamethoxazole (100%) and vancomycin (100%). MRSA appeared co-existing among dogs in the same cage on the basis of the identical MLVF and genotype. In conclusion, high SA isolation rate was found in shelter dogs. se distribution in shelter dogs were different from pet dogs and their owners, and 20% isolates carried food poison associated classical se. MRSA in this shelter study showed similar microbiological characteristics to CA-MRSA ST59 reported in Taiwan and ST508 was also reported.