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標題: | 不同溫度及營養元素對水稻蔗糖轉運蛋白基因表現之影響 Effect of Temperature and Nutrient on the Expression of Rice Sucrose Transporter Genes |
作者: | Ping Chung 鍾萍 |
指導教授: | 王淑珍(Shu-Jen Wang) |
關鍵字: | 蔗糖轉運蛋白,水稻,溫度,銨態氮源,缺氮, sucrose transporter,rice(Oryza sativa L.),temperature,ammonium nitrogen,nitrogen starvation, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | 蔗糖為高等植物醣類長距離運輸的主要形式。蔗糖由細胞間質裝載入韌皮部進行運送及由韌皮部卸載至周圍細胞的過程中,蔗糖轉運蛋白 (sucrose transporters; SUTs) 扮演非常重要的角色。而水稻 (Oryza sativa L.) 蔗糖轉運蛋白基因家族成員有OsSUT1、OsSUT2、OsSUT3、OsSUT4及OsSUT5。本研究之目的為探討溫度及氮源對水稻SUT基因表現之影響。研究結果顯示於種子發芽時期,種子胚部的OsSUT1、OsSUT2及OsSUT4在30℃與37℃處理下的基因表現量較20℃處理下高,而在不同溫度處理下分離胚的OsSUT1、OsSUT2及OsSUT4基因表現則與完整種子胚部的表現不同,推測胚乳可提供調控胚中OsSUT基因表現之因子 (例如醣類)。分析OsSUT2及OsSUT4的啟動子活性,結果顯示只有OsSUT4啟動子活性對溫度之反應與基因表現結果相似。另於水稻花粉成熟過程,15/13℃的低溫處理會抑制水稻花藥中OsSUT1、OsSUT3及OsSUT4的表現,進而影響花藥及花粉粒的澱粉累積。在營養因子之相關試驗顯示,在水稻幼苗時期進行缺氮處理會促進其地上部OsSUT1、OsSUT2及OsSUT4的基因表現。另一方面,根部OsSUT1的基因表現則會隨著銨態氮源濃度增加而明顯上升。 Sucrose is the major carbohydrate for long-distance transport in higher plants. Sucrose transporter (SUT) plays an important role to load sucrose into phloem for further transport, and it is also in charge of sucrose unloading from phloem. Rice SUT gene family are composed of five members (i.e. OsSUT1, OsSUT2, OsSUT3, OsSUT4 and OsSUT5). This study was focused on temperature and nitrogen nutrient effects on OsSUT gene expressions at seed germination, seedling growth and pollen maturation stages. The result showed that expressions of OsSUT1, 2 and 4 in embryos of germinating seeds at 30℃ and 37℃ were higher than that at 20℃. Since the effects of temperature on OsSUT1, 2 and 4 expressions in isolated embryos were different with that in embryos of whole seeds, it was suggested that endosperms might provide some factors such as sugars to regulate OsSUT gene expressions in embryos. In addition, the promoter analysis data showed that the OsSUT4 promoter activity responsed to different temperatures were consistent to the gene expression patterns. During pollen maturation, the expressions of OsSUT1, 3 and 4 in anthers were lower at 15/13℃ compared with that at 30/25℃ and 35/30℃. In the study related to nutrient effect on OsSUT expressions, it was indicated nitrogen starvation would enhance OsSUT1, 2 and 4 expressions in shoots. On the other hand, ammonium function as a positive factor to regulate OsSUT1 expression in root tissues. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63567 |
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顯示於系所單位: | 農藝學系 |
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