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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 丁詩同 | |
dc.contributor.author | Ya-Chin Wang | en |
dc.contributor.author | 王雅青 | zh_TW |
dc.date.accessioned | 2021-05-16T16:25:42Z | - |
dc.date.available | 2014-03-15 | |
dc.date.available | 2021-05-16T16:25:42Z | - |
dc.date.copyright | 2013-03-15 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-02-21 | |
dc.identifier.citation | 1. Yang, R.Z. et al. Acute-phase serum amyloid A: an inflammatory adipokine and potential link between obesity and its metabolic complications. PLoS Med 3, e287 (2006).
2. Trayhurn, P. & Wood, I.S. Adipokines: inflammation and the pleiotropic role of white adipose tissue. Br J Nutr 92, 347-55 (2004). 3. Falcao-Pires, I., Castro-Chaves, P., Miranda-Silva, D., Lourenco, A.P. & Leite-Moreira, A.F. Physiological, pathological and potential therapeutic roles of adipokines. Drug Discov Today 17, 880-9 (2012). 4. Koerner, A., Kratzsch, J. & Kiess, W. Adipocytokines: leptin--the classical, resistin--the controversical, adiponectin--the promising, and more to come. Best Pract Res Clin Endocrinol Metab 19, 525-46 (2005). 5. Vigerust, N.F. et al. Krill oil versus fish oil in modulation of inflammation and lipid metabolism in mice transgenic for TNF-alpha. Eur J Nutr (2012). 6. Wang, Y.C. et al. Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin. J Nutr Biochem 21, 317-24 (2010). 7. Oliver, E., McGillicuddy, F., Phillips, C., Toomey, S. & Roche, H.M. The role of inflammation and macrophage accumulation in the development of obesity-induced type 2 diabetes mellitus and the possible therapeutic effects of long-chain n-3 PUFA. Proc Nutr Soc 69, 232-43 (2010). 8. Finucane, M.M. et al. National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9.1 million participants. Lancet 377, 557-67 (2011). 9. Ofei, F. Obesity - a preventable disease. Ghana Med J 39, 98-101 (2005). 10. Ruzickova, J. et al. Omega-3 PUFA of marine origin limit diet-induced obesity in mice by reducing cellularity of adipose tissue. Lipids 39, 1177-85 (2004). 11. Aguilera, A.A., Diaz, G.H., Barcelata, M.L., Guerrero, O.A. & Ros, R.M. Effects of fish oil on hypertension, plasma lipids, and tumor necrosis factor-alpha in rats with sucrose-induced metabolic syndrome. J Nutr Biochem 15, 350-7 (2004). 12. Lopez-Huertas, E. The effect of EPA and DHA on metabolic syndrome patients: a systematic review of randomised controlled trials. Br J Nutr 107 Suppl 2, S185-94 (2012). 13. Browning, L.M. n-3 Polyunsaturated fatty acids, inflammation and obesity-related disease. Proc Nutr Soc 62, 447-53 (2003). 14. Xu, H. et al. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 112, 1821-30 (2003). 15. Perreault, M. & Marette, A. Targeted disruption of inducible nitric oxide synthase protects against obesity-linked insulin resistance in muscle. Nat Med 7, 1138-43 (2001). 16. Sartipy, P. & Loskutoff, D.J. Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proc Natl Acad Sci U S A 100, 7265-70 (2003). 17. Weisberg, S.P. et al. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112, 1796-808 (2003). 18. Todoric, J. et al. Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids. Diabetologia 49, 2109-19 (2006). 19. Tai, C.C. & Ding, S.T. N-3 polyunsaturated fatty acids regulate lipid metabolism through several inflammation mediators: mechanisms and implications for obesity prevention. J Nutr Biochem 21, 357-63 (2010). 20. Jump, D.B. N-3 polyunsaturated fatty acid regulation of hepatic gene transcription. Curr Opin Lipidol 19, 242-7 (2008). 21. Kunesova, M. et al. The influence of n-3 polyunsaturated fatty acids and very low calorie diet during a short-term weight reducing regimen on weight loss and serum fatty acid composition in severely obese women. Physiol Res 55, 63-72 (2006). 22. Lefils, J., Geloen, A., Vidal, H., Lagarde, M. & Bernoud-Hubac, N. Dietary DHA: time course of tissue uptake and effects on cytokine secretion in mice. Br J Nutr 104, 1304-12 (2010). 23. Hsu, J.M. & Ding, S.T. Effect of polyunsaturated fatty acids on the expression of transcription factor adipocyte determination and differentiation-dependent factor 1 and of lipogenic and fatty acid oxidation enzymes in porcine differentiating adipocytes. Br J Nutr 90, 507-13 (2003). 24. Hsu, J.M., Wang, P.H., Liu, B.H. & Ding, S.T. The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes. J Anim Sci 82, 683-9 (2004). 25. Liu, B.H., Kuo, C.F., Wang, Y.C. & Ding, S.T. Effect of docosahexaenoic acid and arachidonic acid on the expression of adipocyte determination and differentiation-dependent factor 1 in differentiating porcine adipocytes. J Anim Sci 83, 1516-25 (2005). 26. Benatti, P., Peluso, G., Nicolai, R. & Calvani, M. Polyunsaturated fatty acids: biochemical, nutritional and epigenetic properties. J Am Coll Nutr 23, 281-302 (2004). 27. Yu, Y.H. et al. The function of porcine PPARgamma and dietary fish oil effect on the expression of lipid and glucose metabolism related genes. J Nutr Biochem 22, 179-86 (2011). 28. Kralisch, S. et al. Therapeutic perspectives of adipocytokines. Expert Opin Pharmacother 6, 863-72 (2005). 29. Wellen, K.E. & Hotamisligil, G.S. Inflammation, stress, and diabetes. J Clin Invest 115, 1111-9 (2005). 30. Mies, F., Shlyonsky, V., Goolaerts, A. & Sariban-Sohraby, S. Modulation of epithelial Na+ channel activity by long-chain n-3 fatty acids. Am J Physiol Renal Physiol 287, F850-5 (2004). 31. Chang, W.J., Chen, C.H., Cheng, W.T.K. & Ding, S.T. The effects of dietary docosahexaenoic acid enrichment on the expression of porcine genes. Asian-Aust. J. Anim. Sci. 20, 768-774 (2007). 32. Poitou, C. et al. Serum amyloid A: production by human white adipocyte and regulation by obesity and nutrition. Diabetologia 48, 519-28 (2005). 33. Chen, C.H. et al. Serum amyloid A protein regulates the expression of porcine genes related to lipid metabolism. J Nutr 138, 674-9 (2008). 34. Graversen, J.H. et al. The plasminogen binding site of the C-type lectin tetranectin is located in the carbohydrate recognition domain, and binding is sensitive to both calcium and lysine. J Biol Chem 273, 29241-6 (1998). 35. Kluft, C., Los, P. & Clemmensen, I. Calcium-dependent binding of tetranectin to fibrin. Thromb Res 55, 233-8 (1989). 36. Westergaard, U.B., Andersen, M.H., Heegaard, C.W., Fedosov, S.N. & Petersen, T.E. Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator. Eur J Biochem 270, 1850-4 (2003). 37. Sugg, R.M. et al. Argatroban tPA stroke study: study design and results in the first treated cohort. Arch Neurol 63, 1057-62 (2006). 38. Kamper, E.F. et al. Tetranectin levels in patients with acute myocardial infarction and their alterations during thrombolytic treatment. Ann Clin Biochem 35 ( Pt 3), 400-7 (1998). 39. Fuhlendorff, J., Clemmensen, I. & Magnusson, S. Primary structure of tetranectin, a plasminogen kringle 4 binding plasma protein: homology with asialoglycoprotein receptors and cartilage proteoglycan core protein. Biochemistry 26, 6757-64 (1987). 40. Jaquinod, M. et al. Mass spectrometric characterisation of post-translational modification and genetic variation in human tetranectin. Biol Chem 380, 1307-14 (1999). 41. Hogdall, C.K., Christensen, L. & Clemmensen, I. The prognostic value of tetranectin immunoreactivity and plasma tetranectin in patients with ovarian cancer. Cancer 72, 2415-22 (1993). 42. Obrist, P. et al. Aberrant tetranectin expression in human breast carcinomas as a predictor of survival. J Clin Pathol 57, 417-21 (2004). 43. Pfaffl, M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45 (2001). 44. Chen, C.H., Wang, P.H., Liu, B.H., Mersmann, H.J. & Ding, S.T. Serum amyloid A protein regulates the expression of porcine genes related to lipid metabolism. J Nutr. (2008 ). 45. Seo, J.B. et al. Activated liver X receptors stimulate adipocyte differentiation through induction of peroxisome proliferator-activated receptor gamma expression. Mol Cell Biol 24, 3430-44 (2004). 46. Diraison, F. et al. Over-expression of sterol-regulatory-element-binding protein-1c (SREBP1c) in rat pancreatic islets induces lipogenesis and decreases glucose-stimulated insulin release: modulation by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR). Biochem J 378, 769-78 (2004). 47. Knight, B.L. et al. A role for PPARalpha in the control of SREBP activity and lipid synthesis in the liver. Biochem J 389, 413-21 (2005). 48. Marcus, S.L. et al. Diverse peroxisome proliferator-activated receptors bind to the peroxisome proliferator-responsive elements of the rat hydratase/dehydrogenase and fatty acyl-CoA oxidase genes but differentially induce expression. Proc Natl Acad Sci U S A 90, 5723-7 (1993). 49. Poirier, H. et al. Differential involvement of peroxisome-proliferator-activated receptors alpha and delta in fibrate and fatty-acid-mediated inductions of the gene encoding liver fatty-acid-binding protein in the liver and the small intestine. Biochem J 355, 481-8 (2001). 50. Tecles, F. et al. Analytical validation of commercially available methods for acute phase proteins quantification in pigs. Res Vet Sci 83, 133-9 (2007). 51. Yang, R.Z. et al. Acute-phase serum amyloid A: an inflammatory adipokine and potential link between obesity and its metabolic complications. PLoS Med. 3, e287 (2006). 52. Parra, M.D. et al. Porcine acute phase protein concentrations in different diseases in field conditions. J Vet Med B Infect Dis Vet Public Health 53, 488-93 (2006). 53. Stauffer, F., Petrow, E.P., Burgmann, H., Graninger, W. & Georgopoulos, A. Release of TNF alpha and IL6 from human monocytes infected with Mycobacterium kansasii: a comparison to Mycobacterium avium. Infection 22, 326-9 (1994). 54. Ruzek, M.C., Miller, A.H., Opal, S.M., Pearce, B.D. & Biron, C.A. Characterization of early cytokine responses and an interleukin (IL)-6-dependent pathway of endogenous glucocorticoid induction during murine cytomegalovirus infection. J Exp Med 185, 1185-92 (1997). 55. Petersen, A.M. & Pedersen, B.K. The anti-inflammatory effect of exercise. J Appl Physiol 98, 1154-62 (2005). 56. Schindler, R. et al. Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF. Blood 75, 40-7 (1990). 57. Tilg, H., Trehu, E., Atkins, M.B., Dinarello, C.A. & Mier, J.W. Interleukin-6 (IL-6) as an anti-inflammatory cytokine: induction of circulating IL-1 receptor antagonist and soluble tumor necrosis factor receptor p55. Blood 83, 113-8 (1994). 58. Bethin, K.E., Vogt, S.K. & Muglia, L.J. Interleukin-6 is an essential, corticotropin-releasing hormone-independent stimulator of the adrenal axis during immune system activation. Proc Natl Acad Sci U S A 97, 9317-22 (2000). 59. Steensberg, A., Fischer, C.P., Keller, C., Moller, K. & Pedersen, B.K. IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans. Am J Physiol Endocrinol Metab 285, E433-7 (2003). 60. Xing, Z. et al. IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute inflammatory responses. J Clin Invest 101, 311-20 (1998). 61. Verlengia, R. et al. Comparative effects of eicosapentaenoic acid and docosahexaenoic acid on proliferation, cytokine production, and pleiotropic gene expression in Jurkat cells. J Nutr Biochem 15, 657-65 (2004). 62. Fritsche, K.L., Anderson, M. & Feng, C. Consumption of eicosapentaenoic acid and docosahexaenoic acid impair murine interleukin-12 and interferon-gamma production in vivo. J Infect Dis 182 Suppl 1, S54-61 (2000). 63. Schadinger, S.E., Bucher, N.L., Schreiber, B.M. & Farmer, S.R. PPARgamma2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes. Am J Physiol Endocrinol Metab 288, E1195-205 (2005). 64. Ding, S.T., McNeel, R.L. & Mersmann, H.J. Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo. Comp Biochem Physiol B Biochem Mol Biol 123, 307-18 (1999). 65. Festuccia, W.T., Laplante, M., Berthiaume, M., Gelinas, Y. & Deshaies, Y. PPARgamma agonism increases rat adipose tissue lipolysis, expression of glyceride lipases, and the response of lipolysis to hormonal control. Diabetologia 49, 2427-36 (2006). 66. Holm, C. Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Biochem Soc Trans 31, 1120-4 (2003). 67. Path, G. et al. Human breast adipocytes express interleukin-6 (IL-6) and its receptor system: increased IL-6 production by beta-adrenergic activation and effects of IL-6 on adipocyte function. J Clin Endocrinol Metab 86, 2281-8 (2001). 68. van Hall, G. et al. Interleukin-6 stimulates lipolysis and fat oxidation in humans. J Clin Endocrinol Metab 88, 3005-10 (2003). 69. Rahn Landstrom, T., Mei, J., Karlsson, M., Manganiello, V. & Degerman, E. Down-regulation of cyclic-nucleotide phosphodiesterase 3B in 3T3-L1 adipocytes induced by tumour necrosis factor alpha and cAMP. Biochem J 346 Pt 2, 337-43 (2000). 70. Ryden, M. et al. Targets for TNF-alpha-induced lipolysis in human adipocytes. Biochem Biophys Res Commun 318, 168-75 (2004). 71. Fricke, K., Heitland, A. & Maronde, E. Cooperative activation of lipolysis by protein kinase A and protein kinase C pathways in 3T3-L1 adipocytes. Endocrinology 145, 4940-7 (2004). 72. Dalen, K.T. et al. Adipose tissue expression of the lipid droplet-associating proteins S3-12 and perilipin is controlled by peroxisome proliferator-activated receptor-gamma. Diabetes 53, 1243-52 (2004). 73. Gorjao, R. et al. Effect of docosahexaenoic acid-rich fish oil supplementation on human leukocyte function. Clin Nutr 25, 923-38 (2006). 74. Tappia, P.S., Man, W.J. & Grimble, R.F. Influence of unsaturated fatty acids on the production of tumour necrosis factor and interleukin-6 by rat peritoneal macrophages. Mol Cell Biochem 143, 89-98 (1995). 75. Bradley, R.L., Fisher, F.M. & Maratos-Flier, E. Dietary Fatty Acids Differentially Regulate Production of TNF-alpha and IL-10 by Murine 3T3-L1 Adipocytes. Obesity (Silver Spring) (2008). 76. Trebble, T. et al. Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation. Br J Nutr 90, 405-12 (2003). 77. Delarue, J., LeFoll, C., Corporeau, C. & Lucas, D. N-3 long chain polyunsaturated fatty acids: a nutritional tool to prevent insulin resistance associated to type 2 diabetes and obesity? Reprod Nutr Dev 44, 289-99 (2004). 78. Diep, Q.N., Touyz, R.M. & Schiffrin, E.L. Docosahexaenoic acid, a peroxisome proliferator-activated receptor-alpha ligand, induces apoptosis in vascular smooth muscle cells by stimulation of p38 mitogen-activated protein kinase. Hypertension 36, 851-5 (2000). 79. Larter, C.Z. et al. Activation of peroxisome proliferator-activated receptor alpha by dietary fish oil attenuates steatosis, but does not prevent experimental steatohepatitis because of hepatic lipoperoxide accumulation. J Gastroenterol Hepatol 23, 267-75 (2008). 80. Li, J.J., Huang, C.J. & Xie, D. Anti-obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid. Mol Nutr Food Res 52, 631-45 (2008). 81. LaRosa, P.C. et al. Trans-10, cis-12 conjugated linoleic acid causes inflammation and delipidation of white adipose tissue in mice: a microarray and histological analysis. Physiol Genomics 27, 282-94 (2006). 82. Park, Y., Storkson, J.M., Albright, K.J., Liu, W. & Pariza, M.W. Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34, 235-41 (1999). 83. House, R.L., Cassady, J.P., Eisen, E.J., McIntosh, M.K. & Odle, J. Conjugated linoleic acid evokes de-lipidation through the regulation of genes controlling lipid metabolism in adipose and liver tissue. Obes Rev 6, 247-58 (2005). 84. Thorsdottir, I. et al. Randomized trial of weight-loss-diets for young adults varying in fish and fish oil content. Int J Obes (Lond) 31, 1560-6 (2007). 85. Graham, T.E. et al. Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. N Engl J Med 354, 2552-63 (2006). 86. Skoldberg, F. et al. Analysis of antibody reactivity against cysteine sulfinic acid decarboxylase, a pyridoxal phosphate-dependent enzyme, in endocrine autoimmune disease. J Clin Endocrinol Metab 89, 1636-40 (2004). 87. Yang, Q. et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature 436, 356-62 (2005). 88. Vane, J. & Corin, R.E. Prostacyclin: a vascular mediator. Eur J Vasc Endovasc Surg 26, 571-8 (2003). 89. Chavey, C. et al. Matrix metalloproteinases are differentially expressed in adipose tissue during obesity and modulate adipocyte differentiation. J Biol Chem 278, 11888-96 (2003). 90. Tanaka, A.R. et al. ATP binding/hydrolysis by and phosphorylation of peroxisomal ATP-binding cassette proteins PMP70 (ABCD3) and adrenoleukodystrophy protein (ABCD1). J Biol Chem 277, 40142-7 (2002). 91. Kraemer, F.B. & Shen, W.J. Hormone-sensitive lipase: control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis. J Lipid Res 43, 1585-94 (2002). 92. Kim, S.H. et al. Berberine activates GLUT1-mediated glucose uptake in 3T3-L1 adipocytes. Biol Pharm Bull 30, 2120-5 (2007). 93. Clemmensen, I., Petersen, L.C. & Kluft, C. Purification and characterization of a novel, oligomeric, plasminogen kringle 4 binding protein from human plasma: tetranectin. Eur J Biochem 156, 327-33 (1986). 94. Seki, T. et al. Reciprocal regulation of tissue-type and urokinase-type plasminogen activators in the differentiation of murine preadipocyte line 3T3-L1 and the hormonal regulation of fibrinolytic factors in the mature adipocytes. J Cell Physiol 189, 72-8 (2001). 95. Morange, P.E. et al. Influence of t-pA and u-PA on adipose tissue development in a murine model of diet-induced obesity. Thromb Haemost 87, 306-10 (2002). 96. Alessi, M.C., Poggi, M. & Juhan-Vague, I. Plasminogen activator inhibitor-1, adipose tissue and insulin resistance. Curr Opin Lipidol 18, 240-5 (2007). 97. Morange, P.E. et al. Influence of PAI-1 on adipose tissue growth and metabolic parameters in a murine model of diet-induced obesity. Arterioscler Thromb Vasc Biol 20, 1150-4 (2000). 98. Nagase, H. & Woessner, J.F., Jr. Matrix metalloproteinases. J Biol Chem 274, 21491-4 (1999). 99. Vu, T.H. & Werb, Z. Matrix metalloproteinases: effectors of development and normal physiology. Genes Dev 14, 2123-33 (2000). 100. Croissandeau, G., Chretien, M. & Mbikay, M. Involvement of matrix metalloproteinases in the adipose conversion of 3T3-L1 preadipocytes. Biochem J 364, 739-46 (2002). 101. Kubo, Y., Kaidzu, S., Nakajima, I., Takenouchi, K. & Nakamura, F. Organization of extracellular matrix components during differentiation of adipocytes in long-term culture. In Vitro Cell Dev Biol Anim 36, 38-44 (2000). 102. Traurig, M.T. et al. Differential expression of matrix metalloproteinase 3 (MMP3) in preadipocytes/stromal vascular cells from nonobese nondiabetic versus obese nondiabetic Pima Indians. Diabetes 55, 3160-5 (2006). 103. Gomez, D.E., Alonso, D.F., Yoshiji, H. & Thorgeirsson, U.P. Tissue inhibitors of metalloproteinases: structure, regulation and biological functions. Eur J Cell Biol 74, 111-22 (1997). 104. Maquoi, E., Demeulemeester, D., Voros, G., Collen, D. & Lijnen, H.R. Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation. Thromb Haemost 89, 696-704 (2003). 105. Alexander, C.M., Selvarajan, S., Mudgett, J. & Werb, Z. Stromelysin-1 regulates adipogenesis during mammary gland involution. J Cell Biol 152, 693-703 (2001). 106. Kralisch, S. et al. Tissue inhibitor of metalloproteinase-1 predicts adiposity in humans. Eur J Endocrinol 156, 257-61 (2007). 107. O'Hara, A., Lim, F.L., Mazzatti, D.J. & Trayhurn, P. Microarray analysis identifies matrix metalloproteinases (MMPs) as key genes whose expression is up-regulated in human adipocytes by macrophage-conditioned medium. Pflugers Arch 458, 1103-14 (2009). 108. Unoki, H. et al. Macrophages regulate tumor necrosis factor-alpha expression in adipocytes through the secretion of matrix metalloproteinase-3. Int J Obes (Lond) 32, 902-11 (2008). 109. Kopecky, J. et al. n-3 PUFA: bioavailability and modulation of adipose tissue function. Proc Nutr Soc 68, 361-9 (2009). 110. Flachs, P. et al. Polyunsaturated fatty acids of marine origin upregulate mitochondrial biogenesis and induce beta-oxidation in white fat. Diabetologia 48, 2365-75 (2005). 111. Paton, C.M. & Ntambi, J.M. Biochemical and physiological function of stearoyl-CoA desaturase. Am J Physiol Endocrinol Metab 297, E28-37 (2009). 112. Steppan, C.M. et al. The hormone resistin links obesity to diabetes. Nature 409, 307-12 (2001). 113. Spranger, J. et al. Adiponectin and protection against type 2 diabetes mellitus. Lancet 361, 226-8 (2003). 114. El-Wakkad, A., Hassan, N.E., Sibaii, H. & El-Zayat, S.R. Proinflammatory, anti-inflammatory cytokines and adiponkines in students with central obesity. Cytokine (2013). 115. Liu, L.R. et al. Serum amyloid A induces lipolysis by downregulating perilipin through ERK1/2 and PKA signaling pathways. Obesity (Silver Spring) 19, 2301-9 (2011). 116. Tai, C.C. et al. Docosahexaenoic acid enhances hepatic serum amyloid A expression via protein kinase A-dependent mechanism. J Biol Chem 284, 32239-47 (2009). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6310 | - |
dc.description.abstract | 脂肪代謝相關疾病,例如:胰島素阻抗、糖尿病以及心血管疾病嚴重威脅人類健康,而N-3多不飽合脂肪酸,特別是docosahexaenoic acid (DHA)被發現對這些疾病有療效,但其中的作用機制尚未被發覺。先前的研究顯示血液澱粉酶A (SAA)參與DHA在之下脂質代謝的調控,於是我們率先先設計實驗闡明其中的機制。結果顯示,在初代培養的人類脂肪細胞(PHA), DHA處理則增加SAA基因及其它相關脂質代謝基因的表現。另外,SAA重組蛋白的處理,抑制PPARγ及脂質生成基因表現,並且提升脂質分解基因的表現,例如hormone sensitive lipase (HSL),同時伴隨甘油自脂肪細胞釋出的提高。我們更進一步發現,SAA的處理,降低覆膜在油滴表面的perilipin基因表現。此結果說明,DHA和SAA部份增加HSL的基因表現並透過降低perilipin來影響脂解作用。在另一個研究中,利用二維電泳的技術,我們發現一個會與plasminogen結合的蛋白;四締素(tetranectin ;TN),為一種自脂肪細胞外泌的脂肪細胞激素。基因表現分析中顯示,在人類脂肪組織中,TN的表現量與身體質量指數(BMI)和脂質生合成呈正比。利用腺病毒大量表現TN在PHA中,可提高脂質生成相關基因表現及油滴在脂肪細胞中堆積。同樣利用腺病毒感染PHA,降低TN表現的實驗中,則無以上的現象。活體實驗中顯示,相較於對照組,高脂餵飼的小鼠脂肪組織中有大量TN表現。小鼠在DHA的餵飼後,TN表現則顯著受到抑制。綜合以上結果,顯示DHA可調控脂解因子SAA表現上升及並降低促進脂質生成的TN表現,進而調整脂肪組織中的脂質代謝,有益於改善肥胖。從此成果中,可做為未來發展治療肥胖及相關疾病的方向。 | zh_TW |
dc.description.abstract | Obesity is highly associated with insulin resistance, diaabetes and cardiovascular diseases, and thereby greatly impacts human health. n-3 polyunsaturated fatty acids, specifically docosahexaenoic acid (DHA), has beneficial effects on human health, but the underlying mechanisms remain elusive. Based on our previous studies that serum amyloid A protein (SAA) is involved in DHA’s regulation of lipid metabolisms, we then conducted a serious of studies to elucidate the underlying mechanism. Promoted SAA and lipid metabolism-associated gene expression in human adipocytes results showed that treating the primary human adipocytes (PHA) with DHA increases the gene expression of SAA and those genes associated with lipid metabolism. Moreover, addition of recombinant SAA inhibited the expression of peroxisome proliferator-activated receptor γ and lipogenic genes, but enhanced lipolytic gene expression such as hormone sensitive lipase (HSL) in PHA, in association with increased glycerol release into the medium. We also found that expression of perilipin, a lipid droplet-coating protein, was decreased by hSAA1 treatment. These results suggest that the lipolytic effects of DHA and SAA are, at least partially, mediated by upregulation of HSL and perilipin downregulation. Using 2D PAGE technique, a plasminogen -kind protein, tetranectin (TN) was identified as a secretory adipocytokin from PHA. In human adipose tissue, expression of TN is positively correlated with body mass index, as well as adipogenesis. Lentiviral overexpression of TN in PHA increased the expression of lipogenic genes and lipid accumulation, which were blocked by lentiviral knockdown of TN. Finally, we found that TN expression was elevated in the adipose tissues of mice fed with high-fat diet fed, but was diminished by DHA treatment. Taken together, our results suggest that DHA exerts beneficial effects on obesity by up-regulating a lipolytic factor, SAA expression, yet down-regulating lipogenic TN, to modulate lipid metabolism in the adipose tissue. The results gained from the study may shed lights for future development of better therapeutic approaches in obesity and related diseases. | en |
dc.description.provenance | Made available in DSpace on 2021-05-16T16:25:42Z (GMT). No. of bitstreams: 1 ntu-102-F94626003-1.pdf: 3140282 bytes, checksum: a9ae48fd9b2c5313275e8ae054f00f63 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iv List of figures viii Introduction 1 Chapter 1 Literature review 2 Chapter 2 Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin 9 2.1 Aims of research plan 9 2.2 Introduction 9 2.3 Materials and methods 10 2.4 Results 15 2.4.1 Effect of recombinant hSAA1 on the expression of genes related to lipid metabolism in HepG2 cells 15 2.5 Discussion 24 Chapter 3 Tetranectin promotes adipogenesis and lipogenesis and is negatively regulated by polyunsaturated fatty acids in human adipocytes 29 3.1 Aims of research plan 29 3.2 Introduction 29 3.3 Material and methods 30 3.4 Result 36 3.5 Discussion 58 Appendix........................ 64 REFERENCES 65 | |
dc.language.iso | en | |
dc.title | 二十二碳六烯酸藉由血漿澱粉酶A蛋白與四締素調控脂肪細胞脂質分解與堆積 | zh_TW |
dc.title | Docosahexaenoic acid regulates lipolysis and lipogenesis through serum amyloid A protein and tetranectin in adipocytes | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 陳洵一,陳朱亮,葉秀慧,朱有田 | |
dc.subject.keyword | 二十二碳六烯酸,血漿澱粉?A,四締素,脂解作用,脂質生成,脂肪細胞激素, | zh_TW |
dc.subject.keyword | docosahexaenoic acid,serum amyloid A protein,tetranectin,lipolysis,lipogenesis,adipocytokines, | en |
dc.relation.page | 71 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2013-02-21 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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