請用此 Handle URI 來引用此文件：
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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫璐西 | |
| dc.contributor.author | Shih-Ying Pan | en |
| dc.contributor.author | 潘思穎 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:58:40Z | - |
| dc.date.available | 2013-08-14 | |
| dc.date.copyright | 2013-08-14 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-08 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61292 | - |
| dc.description.abstract | 糖尿病是由於缺乏胰島素、胰島素作用不良或是兩者皆有缺陷，而導致高血糖的一種代謝性疾病。約90%的病患屬於第二型糖尿病，其主要的病因為胰島素阻抗，胰島素阻抗是由於周邊組織對於胰島素的反應不良，而使血液中的葡萄糖無法被攝取進入細胞中。台灣藜為藜科草本植物，是台灣的特有種，若不將其外殼去除，則味道苦澀，因此較少被人食用，近年來研究發現台灣藜的營養及機能性成分含量相當豐富，其可能的生理活性也日漸受到重視。本研究使用3T3-L1脂肪細胞進行葡萄糖攝取試驗，探討台灣藜中具調節血糖之活性成分。初步以六種溶劑進行台灣藜脫穀粒及外殼之萃取，結果顯示，外殼之水萃物可以顯著增加細胞的葡萄糖攝取量，因此進一步利用不同極性之溶劑進行區分，發現水層在低濃度就可以展現良好的降血糖活性。接著以XAD-7管柱層析後，分離出兩個主要的活性成分P1及P2。這兩個樣品在5~10 ppm下，就可以展現其降血糖活性。當在10 nM胰島素刺激下，P1具有協同胰島素的作用，而P2則會降低脂肪細胞之葡萄糖攝取量，推測P2可能具有類似胰島素的活性。 | zh_TW |
| dc.description.abstract | Diabetes mellitus is a group of metabolic diseases characterized by high blood sugar resulting from defects in insulin secretion, insulin action or both. Most patients with type 2 diabetes are deficient in glucose uptake by muscle and fat cells due to peripheral insulin resistance. Chenopodium formosanum Koidz. (djulis) is a native pseudocereal plant grown in Taiwan, it possesses numerous nutrients and bioactive components. However, it tastes bitter if the husk is not removed. Mature 3T3-L1 adipocyte was used in this investigation as cell model to study the active hypoglycemic components of djulis. Initially, six solvents with different polarities were used to extract the whole seed or the husk of djulis. Djulis husk water crude extract (DHW) was found to significantly increase glucose uptake of 3T3-L1 cell. After solvent partition of the crude extract, the water layer showed the best glucose uptake activity at low concentrations. There were two components (P1and P2) isolated from the water layer by column chromatography. They showed hypoglycemic activity at 5~10 ppm. Co-treatment with 10 nM insulin, P1 showed synergistic effect with insulin, however, P2 reduced insulin-stimulated glucose uptake. P2 might act as an insulin-mimetic substance. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:58:40Z (GMT). No. of bitstreams: 1 ntu-102-R00641025-1.pdf: 3036567 bytes, checksum: 6fce60d9b028b772ed9b27e9d3130110 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 中文摘要 I
英文摘要 II 縮寫表 III 目錄 IV 圖次 VII 表次 IX 附圖次 X 附表次 XI 壹、前言 1 貳、文獻整理 2 第一節、 材料介紹 2 一、 台灣藜簡介 2 二、 台灣藜成分與生理活性 2 三、 台灣藜之降血糖功效 5 (一) 抗氧化 6 (二) 促進周邊組織之葡萄糖攝取 6 (三) 抑制腸道水解酵素活性 7 四、 藜麥簡介 8 第二節、 糖尿病簡介 9 一、 血糖恆定 9 二、 胰島素 10 三、 葡萄糖轉運蛋白 11 (一) PI3K pathway 11 (二) c-Cbl-CAP pathway 12 四、 糖尿病定義及分類 15 第三節、 3T3-L1脂肪細胞模式 17 一、 脂肪組織與血糖恆定 17 二、 3T3-L1脂肪細胞簡介 19 第四節、 葡萄糖攝取試驗 21 一、 葡萄糖攝取試驗簡介 21 二、 3T3-L1脂肪細胞之葡萄糖攝取試驗 22 參、研究目的與實驗架構 28 第一節、研究目的 28 第二節、實驗架構 29 肆、材料與方法 32 第一節、 實驗材料與儀器設備 32 一、 實驗材料 32 二、 實驗細胞株 32 三、 化學藥品與試劑 32 四、 細胞實驗各培養溶液 34 五、 細胞實驗各種溶液配方 35 六、 層析材料 37 七、 化學分析儀器設備 37 八、 細胞實驗儀器設備 39 九、 實驗耗材 40 第二節、 實驗方法 41 一、 台灣藜外殼之一般組成分分析 41 二、 台灣藜之分離純化 44 (一) 樣品前處理 44 (二) 台灣藜粗萃物之製備 44 1. 水粗萃物之製備 44 2. 其它溶劑粗萃物之製備 44 (三) 台灣藜外殼水粗萃物 (DHW) 之溶劑區分 45 (四) 台灣藜外殼水粗萃物水層(DHW-W)之去除多醣 45 (五) 台灣藜外殼水粗萃物水層之酒精可溶層(W-Et)XAD-7 gel管柱層析 45 1. 管柱充填 (Packing) 45 2. 樣品注入 (Loading) 46 3. 沖提條件 (Eluting) 46 4. 薄層層析法 (Thin layer chromatography, TLC) 46 (六) 台灣藜DHW-W-Et-F2之分析型高效能液相層析 47 (七) 台灣藜DHW-W-Et-F2-2之製備型高效能液相層析 48 (八) 台灣藜DHW-W-Et-F2-2-Ⅱ peak 1(P1)& peak 2(P2)之製備型高效能液相層析 50 三、葡萄糖攝取試驗 50 (一) 前脂肪細胞3T3-L1之培養 50 (二) 細胞繼代及冷凍保存 50 (三) 前脂肪細胞3T3-L1之分化培養 51 (四) 葡萄糖攝取試驗 52 第三節、統計分析 54 伍、結果與討論 55 第一節、台灣藜外殼之一般組成分分析 55 第二節、台灣藜之分離純化 56 一、台灣藜粗萃物之製備 56 二、台灣藜外殼水粗萃物(DHW)之溶劑區分 57 三、台灣藜外殼水粗萃物水層(DHW-W)之酒精沉澱區分 58 四、台灣藜外殼水粗萃物水層之酒精可溶層(DHW-W-Et)之XAD-7管柱層析 59 五、台灣藜外殼DHW-W-Et-F2之高效能液相層析(HPLC) 61 六、台灣藜外殼DHW-W-Et-F2-2之高效能液相層析(HPLC) 64 七、台灣藜外殼DHW-W-Et-F2-2-Ⅱ之高效能液相層析(HPLC) 68 第三節、葡萄糖攝取試驗 71 一、脂肪細胞之分化培養 71 二、台灣藜之調節血糖活性評估 74 陸、結論 96 柒、參考文獻 97 捌、附錄 103 | |
| dc.language.iso | zh-TW | |
| dc.title | 以脂肪細胞探討台灣藜中具調節血糖之活性成分 | zh_TW |
| dc.title | Investigation of the Hypoglycemic Components of Chenopodium formosanum Koidz. by Glucose Uptake Assay in 3T3-L1 Adipocytes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡碧仁,吳亮宜,魏國晉,何其儻,呂廷璋 | |
| dc.subject.keyword | 調節血糖,台灣藜,糖尿病,葡萄糖攝取試驗,3T3-L1脂肪細胞, | zh_TW |
| dc.subject.keyword | Anti-diabetic,Chenopodium formosanum Koidz.,diabetes mellitus,glucose uptake assay,3T3-L1 adipocytes, | en |
| dc.relation.page | 113 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-08 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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