請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6127
標題: | 快速寬頻三倍頻顯微影像系統 High-speed Broadband Third Harmonic Generation Microscopy System |
作者: | Yu-Hsiang Cheng 鄭宇翔 |
指導教授: | 孫啟光 |
關鍵字: | 鉻貴橄欖石雷射,脈衝壓縮,非線性顯微術,倍頻顯微術,活體影像, Cr:Forsterite laser,Pulse compression,Nonlinear microscopy,Harmonic generation microscopy,In vivo imaging, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 以鉻貴橄欖石鎖模雷射(中心波長一千兩百六十奈米)作為激發光源,吾人建立起一套高速非線性顯微影像系統用以協助生醫研究,橫向解析度可達五百奈米,縱向解析度約為兩微米。根據觀測物光學特性不同,可以利用適當的非線性效應例如雙光子激發螢光、二倍頻或三倍頻等來產生影像對比。本影像系統可以二十八幀率紀錄四通道影像(單張影像五百一十二像素見方),並即時套以偽色呈現於電腦螢幕上。此高速系統適用於活體觀測,目前已成功取得活體人體皮膚影像及活體人體口腔黏膜影像。
除了基本的顯微術成像,為了增強非線性信號與激發更寬頻非線性響應,在雷射光進入顯微鏡之前,會經由光纖展寬雷射頻譜,再由稜鏡對進行色散補償及脈衝壓縮。壓縮後之雷射脈衝寬度約為二十四飛秒,波長寬度約為八十二奈米。經此雷射脈衝激發出的寬頻的三倍頻信號會依據波長分割成三通道紀錄下來,藉著分析每一像素在不同波長之信號強度,可以研究觀測物各部位產生非線性極化之頻率響應。 Using a Cr:forsterite mode-locked laser (centered at 1260 nm) as the excitation source, I set up a high-speed nonlinear microscope with sub-micron lateral resolution for biomedical imaging applications. Contrast can come from two-photon excitation fluorescence, second harmonic generation, or third harmonic generation. 4 channel images (512 pixels x 512 pixels) could be recorded at a frame rate of 28 fps and shown with pseudo colors on screen in realtime. This microscopy system was successfully applied to human skin imaging and human oral mucosa imaging in vivo. In order to enhance nonlinear signals and excite broadband harmonic responses, the laser pulses were spectrally broadened by a single-mode fiber and then temporally compressed by a prism pair before entering the microscope. The compressed pulse width was 24 fs and the spectrum bandwidth was 82 nm. The broadband third harmonic signals, excited by the compressed pulses, were divided into 3 channels based on different wavelength sections. By analyzing 3 color images pixel by pixel, the frequency response of the nonlinear susceptibility of the specimen can be studied. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6127 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 光電工程學研究所 |
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ntu-102-1.pdf | 50.57 MB | Adobe PDF | 檢視/開啟 |
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