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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60836
Title: | 氣壓操控液珠3D微流道晶片用於齒舌蘭輪斑病毒樣品輸送、混合與檢測 Pressure-controlled Droplet-based 3D Microfluidic Device for ORSV Sample Deliver, Mixing, and Detection |
Authors: | Huang-Min Chu 朱晃民 |
Advisor: | 沈弘俊(Horn-Jiunn Sheen) |
Keyword: | 實驗室晶片,微流道,齒舌蘭輪斑病毒,多樣本檢測,布朗運動,微粒子追蹤測速儀, Lab-on-a-chip,microfluidic device,ORSV,Multiple sample detection,Brownian motion,μ-PTV, |
Publication Year : | 2013 |
Degree: | 碩士 |
Abstract: | 從1980年代以來生物晶片迅速發展,世界各國大學、研究機構致力於相關技術與生物晶片應用開發,實驗室晶片(Lab-on-a-chip)為一微型化晶片,可以在短時間內同步完成大量分析研究,本研究成功開發氣壓操控液珠3D微流道晶片,以應用於蘭花病毒檢測為例,氣壓操控液珠3D微流道晶片可對齒舌蘭輪斑病毒(ORSV)樣品輸送、混合與檢測,檢測機制是以螢光粒子布朗運動為理論基礎,有別於以往只能檢測單一樣本,使用氣壓操控液珠3D微流道晶片在顯微鏡下以CCD擷取畫面,再以微粒子追蹤測速儀(μ-PTV)針對螢光粒子布朗運動做速度分析,可進行多樣本檢測(Multi-sample detection)縮減檢測時間,判別樣本內是否存在齒舌蘭輪斑病毒外亦能分析出病毒濃度區間,檢驗流程簡單且成本低廉,有機會成為國際上常態使用的蘭花病毒檢驗晶片。 We have successfully developed a pressure-controlled droplet-based 3D microfluidic device on simultaneous multiple sample detection. The device includes sample mixing, delivering and loading and is applied to detecting the ORSV of the samples by measuring nano-particle's Brownian velocities. As the functionalized nanoparticles react with different samples, the motions of these nanoparticles are recorded and then analyzed by micro-PTV. This lab-on-a-chip device owning the capability of multiple sample detection can shrink the investigating time when working on multiple sample detection. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60836 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 應用力學研究所 |
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ntu-102-1.pdf Restricted Access | 4.91 MB | Adobe PDF |
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