請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5938
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫啟光(Chi-Kuang Sun) | |
dc.contributor.author | Hsiang-Yu Chung | en |
dc.contributor.author | 鍾向宇 | zh_TW |
dc.date.accessioned | 2021-05-16T16:18:33Z | - |
dc.date.available | 2015-08-20 | |
dc.date.available | 2021-05-16T16:18:33Z | - |
dc.date.copyright | 2013-08-20 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-14 | |
dc.identifier.citation | [1.1] T. Mulvey, “The electron microscope: The British contribution,” Journal of Microscopy 155 (3), pp. 327-338 (1989).
[1.2] L. Reimer, “Scanning Electron Microscopy: Physics of Image Formation and Microanalysis,” Meas. Sci. Technol. 11, pp. 1826 (2000). [1.3] G. Binnig, H. Rohrer, Ch. Gerber, and E. Weibel, “Surface Studies by Scanning Tunneling Microscopy,” Phys. Rev. Lett. 49 (1), pp. 57–61 (1982). [1.4] M. Minsky, “Microscopy Apparatus,” U.S. Patent no. 3013467 (1957). [1.5] C. J. R. Sheppard and D. M. Shotton, Confocal Laser Scanning Microscopy (Oxford, 1997). [1.6] T.H. Maiman, “Stimulated Optical Radiation in Ruby,” Nature 187, pp. 493-494 (1960). [1.7] W. J. Alford, R. D. Vanderneut, and V. J. Zaleckas, “Laser scanning microscopy,” Proceedings of IEEE 70 (6), pp. 641-651 (1982). [1.8] W. Denk, J.H. Strickler, and W.W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, pp. 73-76 (1990). [1.9] R. Hellwarth, and P. Christensen, “Nonlinear optical microscopic examination of structure in polycrystalline ZnSe,” Optics Communications 12 (3), pp. 318-322 (1974). [1.10] M. D. Duncan, J. Reintjes, and T. J. Manuccia, “Scanning coherent anti-Stokes Raman microscope,” Optics Letters 7 (8), pp. 350-352 (1982). [1.11] K. Konig, P. T. C. So, W. W. Mantulin, and E. Gratton, “Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes,” Optics Letters 22 (2), pp. 135-136 (1997). [1.12] P. A. Franken, A. E. Hill, C. W. Peters, and G. Weinreich, “Generation of Optical Harmonics,” Phys. Rev. Lett. 7, pp. 118–119 (1961). [1.13] N. Bloembergen, R. K. Chang, S. S. Jha, and C. H. Lee, “Optical Second-Harmonic Generation in Reflection from Media with Inversion Symmetry,” Phys. Rev. 174, pp. 813–822 (1968). [1.14] I. Freund, M. Deutsch, and A. Sprecher, “Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon,” Biophysical Journal 50 (4), pp. 693-712 (1986). [1.15] G. Peleg, A. Lewis, M. Linial, and L. M. Loew,” Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites,” Proceedings of the National Academy of Sciences of the United States of America 96, pp. 6700-6704 (1999). [1.16] L. Moreaux, O. Sandre, and J. Mertz, “Membrane imaging by second-harmonic generation microscopy,” J. Opt. Soc. Am. B 17 (10), pp. 1685-1694 (2000). [1.17] A. C. Millard, L. Jin, A. Lewis, and L. M. Loew, “Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells,” Optics Letters 28 (14), pp. 1221-1223 (2003). [1.18] D. A. Dombeck, M. Blanchard-Desce, and W. W. Webb, “Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy,” The Journal of Neuronscience 24, pp. 999-1003 (2004). [1.19] Y.-C. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano,” Second-harmonic tomography of tissues,” Optics Letters 22 (17), pp. 1323-1325 (1997). [1.20] D. A. Dombeck, K. A. Kasischke, H. D. Vishwasrao, M. Ingelsson, B. T. Hyman, and W. W. Webb, “Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy,” Proceedings of the National Academy of Sciences of the United States of America 100, pp. 7081-7086 (2003). [1.21] A. Zoumi, A. Yeh, and B. J. Tromberg, “Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence,” Proceedings of the National Academy of Sciences of the United States of America 99, pp. 11014-11019 (2002). [1.22] W. Mohlera, A. C. Millardb, and P. J. Campagnola, “Secondharmonicgeneration imaging of endogenous structural proteins,” Methods 29, pp. 97-109 (2003). [1.23] W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proceedings of the National Academy of Sciences of the United States of America 100, pp. 7075-7080 (2003). [1.24] P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, “Three-Dimensional High-Resolution Second-Harmonic Generation Imaging of Endogenous Structural Proteins in Biological Tissues,” Biophysical Journal 82, pp. 493-508 (2002). [1.25] S.-W. Chu, I.-H. Chen, T.-M. Liu, C.-K. Sun, S.-P. Lee, B.-L. Lin, P.-C. Cheng, M.-X. Kuo, D.-J. Lin, and H.-L. Liu, “Nonlinear bio-photonic crystal effects revealed with multimodal nonlinear microscopy,” Journal of Microscopy 208 (3), pp. 190-200 (2002). [1.26] S.-W. Chu, S.-Y. Chen, G.-W. Chern, T.-H. Tsai, Y.-C. Chen, B.-L. Lin, and C.-K. Sun, “Studies of χ(2)/χ(3)Tensors in Submicron-Scaled Bio-Tissues by Polarization Harmonics Optical Microscopy,” Biophysical Journal 86 (6), pp. 3914-3922 (2004). [1.27] C.-K. Sun, S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, and H.-J. Tsai, “Higherharmonicgeneration microscopy for developmental biology,” Journal of Structural Biology 147 (1), pp. 19-30 (2004). [1.28] T.-M. Liu, Y.-W. Lee, C.-F. Chang, S.-C. Yeh, C.-H. Wang, S.-W. Chu, and C.-K. Sun.” Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy,” Optics Express 16 (8), pp. 5602-5608 (2008). [1.29] T. Y. F. Tsang, “Optical third-harmonic generation at interfaces,” Phys. Rev. A 52, pp. 4116–4125 (1995). [1.30] Y. Guo, P.-P. Ho, A. Tirksliunas, F. Liu, and R. R. Alfano, “Optical harmonic generation from animal tissues by the use of picosecond and femtosecond laser pulses,” Applied Optics 35 (34), pp. 6810-6813 (1996). [1.31] Y. Barad, H. Eizenberg, M. Horowitz and Y. Silberberg, “Nonlinear scanning laser microscopy by thirdharmonic generation,” Appl. Phys. Lett. 70, pp. 922-924 (1997). [1.32] M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff,” 3D microscopy of transparent objects using third-harmonic generation,” Journal of Microscopy 191 (3), pp. 266–274 (1998). [1.33] J. A. Squier, M. Muller, G. J. Brakenhoff, and K. R. Wilson, “Third harmonic generation microscopy,” Optics Express 3 (9), pp. 315-324 (1998). [1.34] D. Yelin and Y. Silberberg, “Laser scanning third-harmonic-generation microscopy in biology,” Opt. Express 5, pp. 169-175 (1999). [1.35] S.-W. Chu, I-H. Chen, T.-M. Liu, P.-C. Chen, C.-K. Sun, and B.-L. Lin, “Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser,” Optics Letters 26 (23), pp. 1909-1911 (2001). [1.36] C.-K. Sun, S.-W. Chu, S.-P. Tai, S. Keller, U. K. Mishra, and S. P. DenBaars, “Scanning second-harmonicOthird-harmonic generation microscopy of gallium nitride,” Appl. Phys. Lett. 77, pp. 2331-2333 (2000). [2.1] P. N. Prasad, Introduction to Biophotonics (Wiley, 2003). [2.2] M. Born and E. Wolf, Principles of Optics (Pergamon,1975). [2.3] E. Hecht, Optics (Addison Wesley, 2002) [2.4] R. Guenter, Modern Optics (Wiley,1990) [2.5] R. F. Fischer, B. Tadic, Optical system design (McGtaw-Hill, 2000). [2.6] H. A. Haus, Wave and fields in optoelectronics (Prentice-Hall, 1984) [2.7] J. A. Armstrong, N. Bloembergen, J. Ducuing, P. S. Pershan, “Interactions between Light Waves in a Nonlinear Dielectric,” Phys. Rev. 127, pp. 1918–1939 (1962). [2.8] R. W. Boyd, Nonlinear optics (Academic, 1992) [2.9] Y.-R. Shen, The principles of nonlinear optics (Wiley,1984) [2.10] P. Weinberger, 'John Kerr and his Effects Found in 1877 and 1878,' Philosophical Magazine Letters 88 (12), pp. 897–907 [2.11] S. Feng, H. G. Winful, “Physical origin of the Gouy phase shift,” Optics Letters 26 (8), pp. 485-487 (2001). [2.12] D. Debarre, N. Olivier, and E. Beaurepaire, “Signal epidetection in third-harmonic generation microscopy of turbid media,” Optics Express 15 (14), pp. 8913-8924 (2007). [2.13] E. G. Sauter, Nonlinear Optics (John Wiley & Sons). [2.14] G. P. Agrawal, Nonlinear fiber optics (Academic, 2007). [2.15] D. Derickson, Fiber optic test and measurement (Prentice Hall, 1998). [2.16] J. Schutz, W. Hodel, and H.P. Weber, “Nonlinearpulse distortion at the zero dispersion wavelength of an optical fibre,” Optics Communications 95 (4–6), pp. 357–365 (1993). [2.17] V. P. Yanovsky and F. W. Wise, “Nonlinear propagation of high-power, sub-100-fs pulses near the zero-dispersion wavelength of an optical fiber,” Optics Letters. 19 (19), pp. 1547-1549 (1994). [3.1] T. H. Maiman, “Stimulated Optical Radiation in Ruby,” Nature 187, pp. 493-494 (1960). [3.2] N. Taylor, LASER: The inventor, the Nobel laureate, and the thirty-year patent war (Simon & Schuster, 2000). [3.3] W. E. Lamb, Jr., 'Theory of an optical laser,' Phys. Rev. 134, pp. 1429-1450 (1964). [3.4] F. J. McClung and R. W. Hellwarth, “Giant Optical Pulsations from Ruby,” Applied Optics 1 (1), pp. 103-105 (1962). [3.5] L. E. Hargrove, R. L. Fork, and M. A. Pollack, “Locking of He-Ne laser modes induced by synchronous intracavity modulation,” Appl. Phys. Letters 5, pp. 4-5 (1964). [3.6] H. W. Mocker and R. J. Collins, “Mode competition and self-locking effects in a Q-switched ruby laser,” Appl. Phys. Lett. 7, pp. 270–273 (1965). [3.7] D. J. Kuizenga and A. E. Siegman, “FM and AM mode locking of the homogeneous laser – Part I: theory,” IEEE J. Quantum Electron. 6, pp. 694-708 (1970). [3.8] A. J. DeMaria, D. A. Stetser, and H. Heynau, “Self mode-locking of lasers with saturable absorbers,” Appl. Phys. Lett. 8, pp. 174 (1966). [3.9] D. E. Spence, P. N. Kean, and W. Sibbett, “60-fsec pulse generation from a self-mode-locked Ti:sapphire laser,” Optics Letters 16 (1), pp. 42-44 (1991). [3.10] P. C. Cheng, S. J. Pan, A. Shih, K. S. Kim, W. S. Liou and M. S. Park, “Highly efficient upconverters for multiphoton fluorescence microscopy,” Journal of Microscopy 189: 199–212 (1998). [3.11] M.-C. Chan, T.-M. Liu, S.-P. Tai, and C.-K. Sun, “Compact fiber-delivered Cr:forsterite laser for nonlinear light microscopy,” Journal of Biomedical Optics 10 (4) 054006 (2005). [3.12] S.-W. Chu, I.-H. Chen, T.-M. Liu, C.-K. Sun, S.-P. Lee, B.-L. Lin, P.-C. Cheng, M.-X. Kuo, D.-J. Lin, and H.-L. Liu, “Nonlinear bio-photonic crystal effects revealed with multimodal nonlinear microscopy,” Journal of Microscopy 208 (3), pp. 190–200 (2002). [3.13] R. R. Anderson and J. A. Parrish, “The Optics of Human Skin,” Journal of Investigative Dermatology 77, pp. 13–19 (1981). [3.14] T. Vo-Dinh, Biomedical photonics handbook (CRC Press, 2003). [3.15] P. F. Moulton, “Spectroscopic and laser characteristics of Ti:Al2O3,” JOSA B 3 (1), pp. 125-133 (1986). [3.16] J. A. Giordmaine and Robert C. Miller, 'Tunable Coherent Parametric Oscillation in LiNbO3 at Optical Frequencies,” Phys. Rev. Lett. 14 (24), pp. 973–976 (1965). [3.17] V. Petricevic, “Laser action in chromium-doped forsterite,” Appl. Phys. Lett. 52 (13), pp. 1040-1042 (1988). [3.18] A. Agnesi, E. Piccinini, G.C. Reali, “Influence of thermaleffects in Kerr-lens mode-locked femtosecond Cr4+:forsterite lasers,” Optics Communications 135, Issues (1–3), pp. 77–82 (1997). [3.19] C. Honninger, R. Paschotta, M. Graf, F. Morier-Genoud, G. Zhang, M. Moser, S. Biswal, J. Nees, A. Braun, G.A. Mourou, I. Johannsen, A. Giesen, W. Seeber, and U. Keller, “Ultrafast ytterbium-doped bulk lasers and laser amplifiers,” Applied Physics B: Lasers and Optics 69 (1), pp. 3-17 (1999). [3.20] W. F. Krupke, “Ytterbium solid-state lasers. The first decade,” IEEE J. Sel. Top. Quantum Electron. 6 (6), pp.1287-1296 (2000). [3.21] Y.-W. Tzeng, Y.-Y. Lin, C.-H. Huang, J.-M. Liu, H.-C. Chui, H.-L. Liu, J. M. Stone, J. C. Knight, and S.-W. Chu, “Broadband tunable optical parametric amplification from a single 50 MHz ultrafast fiber laser,” Optics Express 17 (9), pp. 7304-7309 (2009). [3.22] C. L. Hoy, N. J. Durr, P. Chen, W. Piyawattanametha, H. Ra, O. Solgaard, and A. Ben-Yakar, “Miniaturized probe for femtosecond laser microsurgery and two-photon imaging,” Optics Express 16 (13), pp. 9996-10005 (2008). [3.23] C. L. Hoy, O. Ferhanoğlu, M. Yildirim, W. Piyawattanametha, H. Ra, O. Solgaard, and A. Ben-Yakar, “Optical design and imaging performance testing of a 9.6-mm diameter femtosecond laser microsurgery probe,” Optics Express 19 (11), pp. 10536-10552 (2011). [3.24] T.-M. Liu, M.-C. Chan, I.-H. Chen, S.-H. Chia, and C.-K. Sun, “Miniaturized multiphoton microscope with a 24Hz frame-rate,” Optics Express 16 (14), pp. 10501-10506 (2008). [3.25] S.-H. Chia, C.-H. Yu, C.-H. Lin, N.-C. Cheng, T.-M. Liu, M.-C. Chan, I-H. Chen, and C.-K. Sun, “Miniaturized video-rate epi-third-harmonic- generation fiber-microscope,” Optics Express 18 (16), pp. 17382-17391 (2010). [3.26] M. Chen, C. Xu, and W. W. Webb, “Endoscope Lens with Dual Field of View and Resolution for Multiphoton Imaging,” Optics Letters 35 (16), pp. 2735-2737 (2010). [3.27] D. R. Rivera, C. M. Brown, D. G. Ouzounov, I. Pavlova, D. Kobat, W. W. Webb, and C. Xu, “Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue,” Proceedings of the National Academy of Sciences of the United States of America 108 (43), pp. 17598-17603 (2011). [3.28] C. J. Engelbrecht, R. S. Johnston, E. J. Seibel, and F. Helmchen, “Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo,” Optics Expressl 16 (8), pp. 5556-5564 (2008). [3.29] F. Bortoletto1, C. Bonoli1, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, “Multiphoton Fluorescence Microscopy with GRIN Objective Aberration Correction by Low Order Adaptive Optics,” PLoS ONE 6 (7): e22321 (2011). [3.30] F. Helmchen, M. S. Fee1, D. W. Tank, and W. Denk, “A Miniature Head-Mounted Two-Photon Microscope: High-Resolution Brain Imaging in Freely Moving Animals,” Neuron 31 (6), pp. 903–912 (2001). [3.31] F. Helmchena, and W. Denk, “New developments in multiphoton microscopy,” Current Opinion in Neurobiology 12 (5), pp. 593-601 (2002). [3.32] W. Gobel, J. N. D. Kerr, A. Nimmerjahn, and F. Helmchen, “Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective,” Optics Letters 29 (21), pp. 2521-2523 (2004). [3.33] J. Knittel, L. Schnieder, G. Buess, B. Messerschmidt, and T. Possner, “Endoscope-compatible confocal microscope using a gradient index-lens system,” Optics Communications 188 (5–6), pp. 267–273 (2001). [3.34] R. Le Harzic, M. Weinigel, I. Riemann, K. Konig, and B. Messerschmidt, “Nonlinear optical endoscope based on a compact two axes piezo scanner and a miniature objective lens,” Optics Express 16 (25), pp. 20588-20596 (2008). [3.35] R. Le Harzic, I. Riemann, M. Weinigel, K. Konig, and B. Messerschmidt, “Rigid and high-numerical-aperture two-photon fluorescence endoscope,” Applied Optics 48 (18), pp. 3396-3400 (2009). [3.36] Juergen C. Jung and Mark J. Schnitzer, “Multiphoton endoscopy,” Optics Letters 28 (11), pp. 902-904 (2003). [3.37] J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, “In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence Microendoscopy,” Journal of Neurophysiology 92 (5), pp. 3121-3133 (2004). [3.38] B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, “In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope,” Optics Letters 30 (17), pp. 2272-2274 (2005). [3.39] E. D. Cocker, R. P. J. Barretto, J. C. Jung, B. A. Flusberg, H. Ra, O. Solgaard, and M. J. Schnitzer, “A Portable Two-photon Fluorescence Microendoscope Based on a Two-dimensional Scanning Mirror,” Optical MEMS and Nanophotonics, 2007 IEEE/LEOS International Conference, pp. 6-7 (2007). [3.40] W. Piyawattanametha, E. D. Cocker, L. D. Burns, R. P. J. Barretto, J. C. Jung, H. Ra, O. Solgaard, and M. J. Schnitzer, “In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror,” Opt Lett. 34 (15), pp. 2309–2311 (2009). [3.41] R. P. J. Barretto, B. Messerschmidt, and M. J. Schnitzer, “In vivo fluorescence imaging with high-resolution microlenses,” Nature Methods 6, pp. 511 - 512 (2009). [3.42] R.P. Barretto, and M.J. Schnitzer, “In Vivo Optical Microendoscopy for Imaging Cells Lying Deep within Live Tissue,' Imaging: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 2011). [3.43] T. A. Murray, and M. J. Levene, “Singlet gradient index lens for deep in vivo multiphoton microscopy,” J. Biomed. Opt. 17 (2), 021106 (2012). [3.44] L. Fu, A. Jain, H. Xie, C. Cranfield, and M. Gu, “Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror,” Optics Express 14 (3), pp. 1027-1032 (2006). [3.45] L. Fu, A. Jain, C, Cranfield, H. Xie, and M. Gu, “Three-dimensional nonlinear optical endoscopy,” J. Biomed. Opt. 12 (4), 040501 (2007). [3.46] H. Bao, J. Allen, R. Pattie, R. Vance, and M. Gu, “Fast handheld two-photon fluorescence microendoscope with a 475 μm × 475 μm field of view for in vivo imaging,” Optics Letters 33 (12), pp. 1333-1335 (2008). [3.47] Y. Zhao, H. Nakamura, and R. J. Gordon, “Development of a versatile two-photon endoscope for biological imaging,” Biomedical Optics Express 1 (4), pp. 1159-1172 (2010). [3.48] W. Jung, S. Tang, D. T. McCormic, T. Xie, Y.-C. Ahn, J. Su, I. V. Tomov, T. B. Krasieva, B. J. Tromberg, and Z. Chen, “Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy,” Optics Letter 33 (12), pp. 1324-1326 (2008). [3.49] S. Tang, W. Jung, D. McCormick, T. Xie, J. Su, Y.-C. Ahn, B. J. Tromberg and Z. Chen, “Design and implementation of fiber-based multiphoton endoscopy with microelectromechanical systems scanning,” J. Biomed. Opt. 14 (3), 034005 (2009). [3.50] J. Sawinskia and W. Denk, “Miniature random-access fiber scanner for in vivo multiphoton imaging,” Journal of Applied Physics 102 (3), 034701 (2007). [3.51] M. T. Myaing, D. J. MacDonald, and X. Li, “Fiber-optic scanning two-photon fluorescence endoscope,” Optics Letters 31 (8), pp. 1076-1078 (2006). [3.52] Y. Wu, Y. Leng, J. Xi, and X. Li, “Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues,” Optics Express 17 (10), pp. 7907-7915 (2009). [3.53] Y. Wu, J. Xi, M. J. Cobb, and X. Li, “Scanning fiber-optic nonlinear endomicroscopy with miniature aspherical compound lens and multimode fiber collector,” Optics Letters 34 (7), pp. 953-955 (2009). [3.54] Y. Wu, Y. Zhang, J. Xi, M.-J. Li, and X. Li, “Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation,” J. Biomed. Opt. 15 (6), 060506 (2010). [3.55] K. Murari, Y. Zhang, S. Li, Y. Chen, M.-J. Li, and X. Li, “Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm,” Optics Letters 36 (7), pp. 1299-1301 (2011). [3.56] W. Liang, K. Murari, Y. Zhang, Y. Chen, M.-J Li, and X. Li, “Increased illumination uniformity and reduced photodamage offered by the Lissajous scanning in fiber-optic two-photon endomicroscopy,” J. Biomed. Opt. 17 (2), 021108 (2012). [3.57] J. Xi, Y. Chen, Y. Zhang, K. Murari, M.-J. Li, and X. Li, “Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging,” Optics Letters 37 (3), pp. 362-364 (2012). [3.58] Y. Zhang, K. Murari, W. Liang, J. Xi, Y. Chen, M.-J. Li, Z. Bhujwalla, K.Glunte, and X. Li, “Scanning Nonlinear Endomicroscopy Technology for Intrinsic Imaging of Biological Tissues,” CLEO: Applications and Technology, paper: ATh5A.1 (2012). [3.59] G. P. Agrawal, Nonlinear fiber optics (Academic, 2007). [3.60] M. E. Fermann, “Single-mode excitation of multimode fibers with ultrashort pulses,” Optics Letters 23 (1), pp. 52-54 (1998). [3.61] D. G. Ouzounov, K. D. Moll, M. A. Foster, W. R. Zipfel, W. W. Webb, and A. L. Gaeta, “Delivery of nanojoule femtosecond pulses through large-core microstructured fibers,” Optics Letters 27 (17), pp. 1513-1515 (2002). [3.62] F. Helmchen, D. W. Tank, and W. Denk, “Enhanced Two-Photon Excitation Through Optical Fiber by Single-Mode Propagation in a Large Core,” Applied Optics 41 (15), pp. 2930-2934 (2002). [3.63] W. Gobel, A. Nimmerjahn, and F. Helmchen, “Distortion-free delivery of nanojoule femtosecond pulses from a Ti:sapphire laser through a hollow-core photonic crystal fiber,” Optics Letters 29 (11), pp. 1285-1287 (2004). [3.64] R.E. Fischer, B. Tadic-Galeb, Optical system design (McGraw-Hill, 2000) [3.65] J. Squier, M. Muller, “High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging,” Rev. Sci. Instrum. 72, pp. 2855-2867 (2001). [4.1] C. Pruss, E. Garbusi, and W. Osten, “Testing aspheres,” Optics and Photonics News 19 (4), pp. 24-29 (2008). [4.2] S. W. Smith, The Scientist & Engineer's Guide to Digital Signal Processing (California Technical Pub, 1997). [4.3] M. Offroya, Y. Roggob, and L. Duponchel, “Increasing the spatial resolution of near infrared chemical images (NIR-CI): The super-resolution paradigm applied to pharmaceutical products,” Chemometrics and Intelligent Laboratory Systems 117, pp. 183-188 (2012). [4.4] J. Squier, and M. Muller, “High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging,” Rev. Sci. Instrum. 72, pp. 2855-2867 (2001). [4.5] D. C. Brown, “Decentering distortion of lenses,” Photogrammetric Engineering 32 (3), pp. 444-462 (1966). [4.6] J. P. de Villiers, F. W. Leuschner, and R. Geldenhuys, “Centi-pixel accurate real-time inverse distortion correction,” International Symposium on Optomechatronic Technologies. SPIE (2008). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5938 | - |
dc.description.abstract | 非線性光學顯微術的發展已經有許多年了。其相對於傳統顯微鏡較為優秀的光學虛擬切片能力,來自於其光學機制-非線性訊號僅限於物鏡焦點的附近才能產生。該顯微術所使用的光源通常為近紅外光,相對於一般可見光不只對於生物體擁有較低的破壞性,更有較佳的穿透深度。典型非線性光學顯微系統的架設是直接將一般的顯微鏡與雷射光源和額外的掃瞄機制整合在一起,然而這將使的整個系統顯得笨重許多。
為使非線性光學顯微術在臨床上能更方便應用,則原本笨重的系統就必須被縮小、重新設計。在本論文中,我們研究的重心將會擺在微小化的鏡頭上。在我們的藍圖中,一個微小化的觀測鏡頭是必要的,因為一個手持大小的系統在觀測過程中可以擁有更多方便性及彈性。再者,我們希望這個鏡頭能提供寬廣的觀測視野,這樣在觀察的過程中我們便可同時揭露更多資訊;系統的掃描機制如果能夠提供夠高的畫面更新率,則在觀察過程中微小震動造成影像模糊的問題可以獲得改善,甚至可以進行動態影像觀察的應用。 非球面鏡名稱的由來在於其表面並非剛好是正球型的一部份。相對於一般簡易生產的球面鏡,其較複雜的表面是專門設計用來減少像差或是取代多重鏡組。一些非球面鏡被設計的非常小,並被應用在手機相機鏡頭、光碟機讀取頭或是雷射二極體準直鏡上。這些鏡子的尺寸都不大而且價錢也相當便宜,或許可以用來作為傳統顯微物鏡的替代品並發揮類似的功能。 在本論文中,我們將呈現我們對於高數值孔徑微小非球面鏡(0.85 NA 藍光光碟機鏡頭、0.8 NA 雷射二極體準直鏡)作為微型化非線性光學顯微系統物鏡的潛力之研究。本系統的構造非常簡單,只有5片鏡子。微小非球面鏡與不同放大倍率之筒鏡被組合起來當作系統的物鏡,微機電鏡擔任系統掃描機制的角色,一片二色的分光鏡則用來區隔入射的紅外光與反射的可見光訊號。我們研究了該系統在不同非線性光學訊號上之表現,諸如:雙光子螢光、二倍頻、三倍頻,並使用綠螢光斑馬魚來評估生物活體實驗的可行性,以及動態影像的觀察。在論文中,亦涵蓋了本設計與其他不同微小化系統設計的比較。 | zh_TW |
dc.description.abstract | Nonlinear optical microscopy has been developed for many years. Due to the mechanism of nonlinear optics, the nonlinear signal can only be generated around the focus of the objective. Thus, this kind of microscopy is known for its optical virtual biopsy ability in comparison with the traditional wide field microscopy. The excitation light is infrared, which is not only less invasive but also provides better depth of penetration. Typical way to apply nonlinear optical microscopy is directly integrating a conventional microscope with a laser light source and some additional scanning mechanisms. However, it will make the whole system bulkier.
In order to apply nonlinear optical microscopy in clinical applications, the system must be miniaturized and redesigned for more flexibility. In this thesis, the investigation is focused on the miniaturized imaging head. An optical imaging head with a miniaturized size, a larger field of view (FOV), and a video frame-rate is highly desired because a miniaturized system is more convenient to be manipulated during the observation and allows intravital applications. Larger field of view means we can reveal more information once simultaneously. Higher frame rate can not only deal with the image blurring problem resulted from vibrations but also allow one to reduce the imaging acquisition time, thus dynamic observation may be realized. Aspheric lenses, which are known for their complex lens surface profile designed for aberration reduction or replacement for a multi-lens system, are used in 3C products, such as cell phone cameras, optical disk drives, or laser diode collimators. With its smaller size and cheaper price, it could be an alternative to traditional objectives in miniaturized nonlinear microscopy systems. In this thesis, we present our investigation on the potential to use high numerical aperture mini aspheric lens (a blu-ray disk lens with 0.85 NA and a laser diode collimating lens with 0.8 NA) as the objective of the miniaturized nonlinear microscopy system. The structure of the system is very simple, and only five mirrors or lenses are used. The mini aspheric lens is integrated with a tube lens pair for beam size magnification. A MEMS mirror acts as a scanner, and a dichroic beam splitter separates the excitation light and the epi-collected signal. We investigate its performance for 2PF (two-photon fluorescence), SHG (second harmonic generation), and THG (third harmonic generation) microscopies. Live GFP (green fluorescence protein) zebrafish is used to estimate the feasibility of in-vivo experiment and the ability of dynamic observation. Comparison among different systems from other groups is also listed. | en |
dc.description.provenance | Made available in DSpace on 2021-05-16T16:18:33Z (GMT). No. of bitstreams: 1 ntu-102-R99941075-1.pdf: 3731858 bytes, checksum: 3a1893dcc207f3c945c89a6a585f3181 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 誌謝 i
摘要 ii ABSTRACT iv CONTENTS vi LIST OF FIGURES ix LIST OF TABLES xv Chapter 1 Introduction 1 1.1 Historical overview of microscopy 1 1.2 Structure of this thesis 4 Reference 6 Chapter 2 Basic principles 11 2.1 Basic conceptions of microscopy 11 2.1.1 Resolution 11 2.1.2 Aberration 12 2.2 Nonlinear optics 14 2.2.1 Second harmonic generation (SHG) 14 2.2.2 Third harmonic generation (THG) 16 2.2.3 Two-photon fluorescence (2PF) 17 2.3 Phenomena in optical fibers 20 2.3.1 Dispersion 20 2.3.2 Nonlinearity and higher order dispersion 23 Reference 27 Chapter 3 Nonlinear optical microscopy 29 3.1 Basic components of a nonlinear optical microscope 29 3.1.1 Pulsed laser 29 3.2 Selection of the light source 31 3.3 Common light source 32 3.3.1 Ti:sapphire laser 32 3.3.2 Cr:forsterite laser 33 3.3.3 Yb fiber laser 33 3.4 Miniaturization of the nonlinear optical microscope 34 3.4.1 GRIN lens 35 3.4.2 Optical fiber 36 3.4.3 Mini scanner 37 3.5 Comparison between different miniaturized systems 37 Reference 44 Chapter 4 Mini aspheric lens applied in the nonlinear optical microscope 52 4.1 Method of miniaturization 52 4.1.1 Tube lens pair design 53 4.1.2 Aspheric lens 55 4.2 Package of the system and experimental setup 57 4.2.1 Repackage of the system 58 4.3 Electronic control and instant data processing 62 4.4 Performance 63 4.4.1 Field of view (FOV) estimation 64 4.4.2 Resolution estimation 67 4.4.3 Performance discussion 71 4.4.4 Simulation of the tube lens 74 4.4.5 Distortion aberration 81 4.5 Application 84 4.5.1 Nonlinear optical sample image 85 4.5.2 Real-time zebrafish heartbeat observation 91 Reference 95 Chapter 5 Summary & future work 96 | |
dc.language.iso | en | |
dc.title | 微小非球面鏡於微型化視頻非線性光學顯微鏡之應用 | zh_TW |
dc.title | The Application of Mini Aspheric Lens in Miniaturized Video-rate Nonlinear Optical Microscope | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 高甫仁(Fu-Jen Kao),宋孔彬(Kung-Bin Sung) | |
dc.subject.keyword | 雷射,非球面鏡,微機電鏡,非線性光學顯微術, | zh_TW |
dc.subject.keyword | Laser,Aspheric lens,MEMS mirror,Nonlinear optical microscopy, | en |
dc.relation.page | 95 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2013-08-15 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
顯示於系所單位: | 光電工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf | 3.64 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。