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標題: | 阿拉伯芥中FIN219和FIN219-interacting Protein 2 (FIP2)的交互作用在茉莉酸、離層酸和光訊息傳遞中的功能性研究 Functional Studies of FIN219 and FIN219-interacting Protein 2 (FIP2) Interaction in the Integration of Jasmonate, Abscisic Acid and Light Signaling in Arabidopsis |
作者: | Chin-Wen Chang 張槿玟 |
指導教授: | 謝旭亮(Hsu-Liang Hsieh) |
關鍵字: | 光,離層酸,茉莉酸, Jasmonate,Abscisic Acid,Light, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 光是一個重要的環境因子,影響植物整個生長與發育。而茉莉酸與離層酸為重要的植物荷爾蒙,在植物生命週期中及對抗環境逆境都扮演著重要的角色。植物要如何將外部的光源與內源的離層酸與茉莉酸訊息調控機制做整合,至今為止尚未明瞭。在阿拉伯芥中,已知FIN219為遠紅光訊息傳遞下的正調控者,並可促進JA-Ile的生合成,進而影響茉莉酸訊息調控。實驗室前人利用酵母菌雙雜交法(yeast two-hybrid)找出與FIN219具有交互作用的蛋白質,命名為FIN219-interacting protein 2(FIP2),即為glutamyl-tRNA synthetase(GluRS)。在前人的研究當中已知FIP2/GluRS會與ABA INSENSITIVE 1 (ABI1)以及Homeodomain protein 6(AtHB6)有交互作用。在本篇研究裡,我們利用探討FIN219與FIP2在遠紅光下如何共同調節茉莉酸與離層酸的訊息,幫助了解植物如何整合協調外部光源與內生荷爾蒙的訊息傳遞。我們首先證實FIN219與FIP2在菸草與阿拉伯芥中都有交互作用,而FIN219是利用C端與FIP2進行結合。而在遠紅光中處理MeJA的情況下,FIP2與FIN219之間有相互抑制的現象。另外,FIP2基因表現量下降的轉殖株在遠紅光下同時處理茉莉酸與離層酸,與野生型相比,會呈現較長的下胚軸之外表型。利用電子顯微鏡還觀察到FIP2缺失或大量表現的植株,其原生質體及葉綠體的發育均受到影響。當FIP2大量表現時,會使植株不同部位的組織對荷爾蒙反應出現差異:其氣孔對茉莉酸與離層酸的處理較不敏感,但根部對離層酸卻是非常敏感。而大量表現FIN219則使植株對茉莉酸和離層酸的處理變得較為敏感,氣孔大小較野生型來的小;綜合上述結果顯示FIP2不但可與FIN219互相調節參與遠紅光的訊息路徑,還協調茉莉酸和離層酸的訊息傳遞,整合了光、茉莉酸和離層酸信號傳導。 Light is one of the most important environmental factors regulating multiple aspects of plant growth and development. Abscisic acid (ABA) and jasmonate (JA) are plant hormones that play important roles during many phases of the plant life cycle and in plant responses to various environmental stresses. How plants integrate the external light signal and endogenous ABA and JA pathways for better adaptation to environmental stresses remains unknown. Far-red insensitive 219 (FIN219/JAR1) has been shown to be a positive signal component in FR signaling pathway. To further understand the function of FIN219 in light signaling during Arabidopsis development, a yeast two-hybrid approach has been used to isolate FIN219-interacting partners. A gene FIP2 (FIN219-interacting protein 2) encoding a glutamyl-tRNA synthetase (GluRS) was isolated. However, its function is not completely understood although previous studies showed that GluRS interacted with ABA INSENSITIVE 1 (ABI1) and Homeodomain protein 6 (AtHB6). By Co-IP Assays, we demonstrate a physical interaction between the full-length FIN219 and FIP2 in Nicotiana benthamiana, and this interaction occurred via the C terminus of FIN219 and the FIP2 in Arabidopsis thaliana. Besides, under FR and MeJA treatments, FIN219 and FIP2 can negatively regulate each other. Furthermore, the fip2 mutant shows insensitivity in hypocotyl growth to MeJA and ABA under FR light. Moreover, FIP2 overexpression results in the insensitivity of stomatal opening to MeJA and ABA. Interestingly, both FIP2 knocked-down mutant and overexpressor lines showed a defect in the number and the development of chloroplasts. Taken together, these data indicate that FIP2 may function via different mechanisms from shoot to root in ABA signaling and participate in the integration of FR light, JA and ABA signaling in Arabidopsis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56700 |
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顯示於系所單位: | 植物科學研究所 |
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