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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮(Hsu-Liang Hsieh) | |
dc.contributor.author | Chin-Wen Chang | en |
dc.contributor.author | 張槿玟 | zh_TW |
dc.date.accessioned | 2021-06-16T05:43:00Z | - |
dc.date.available | 2019-08-16 | |
dc.date.copyright | 2014-08-16 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-11 | |
dc.identifier.citation | Abe, H., Urao, T., Ito, T., Seki, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2003). Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15: 63-78.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56700 | - |
dc.description.abstract | 光是一個重要的環境因子,影響植物整個生長與發育。而茉莉酸與離層酸為重要的植物荷爾蒙,在植物生命週期中及對抗環境逆境都扮演著重要的角色。植物要如何將外部的光源與內源的離層酸與茉莉酸訊息調控機制做整合,至今為止尚未明瞭。在阿拉伯芥中,已知FIN219為遠紅光訊息傳遞下的正調控者,並可促進JA-Ile的生合成,進而影響茉莉酸訊息調控。實驗室前人利用酵母菌雙雜交法(yeast two-hybrid)找出與FIN219具有交互作用的蛋白質,命名為FIN219-interacting protein 2(FIP2),即為glutamyl-tRNA synthetase(GluRS)。在前人的研究當中已知FIP2/GluRS會與ABA INSENSITIVE 1 (ABI1)以及Homeodomain protein 6(AtHB6)有交互作用。在本篇研究裡,我們利用探討FIN219與FIP2在遠紅光下如何共同調節茉莉酸與離層酸的訊息,幫助了解植物如何整合協調外部光源與內生荷爾蒙的訊息傳遞。我們首先證實FIN219與FIP2在菸草與阿拉伯芥中都有交互作用,而FIN219是利用C端與FIP2進行結合。而在遠紅光中處理MeJA的情況下,FIP2與FIN219之間有相互抑制的現象。另外,FIP2基因表現量下降的轉殖株在遠紅光下同時處理茉莉酸與離層酸,與野生型相比,會呈現較長的下胚軸之外表型。利用電子顯微鏡還觀察到FIP2缺失或大量表現的植株,其原生質體及葉綠體的發育均受到影響。當FIP2大量表現時,會使植株不同部位的組織對荷爾蒙反應出現差異:其氣孔對茉莉酸與離層酸的處理較不敏感,但根部對離層酸卻是非常敏感。而大量表現FIN219則使植株對茉莉酸和離層酸的處理變得較為敏感,氣孔大小較野生型來的小;綜合上述結果顯示FIP2不但可與FIN219互相調節參與遠紅光的訊息路徑,還協調茉莉酸和離層酸的訊息傳遞,整合了光、茉莉酸和離層酸信號傳導。 | zh_TW |
dc.description.abstract | Light is one of the most important environmental factors regulating multiple aspects of plant growth and development. Abscisic acid (ABA) and jasmonate (JA) are plant hormones that play important roles during many phases of the plant life cycle and in plant responses to various environmental stresses. How plants integrate the external light signal and endogenous ABA and JA pathways for better adaptation to environmental stresses remains unknown. Far-red insensitive 219 (FIN219/JAR1) has been shown to be a positive signal component in FR signaling pathway. To further understand the function of FIN219 in light signaling during Arabidopsis development, a yeast two-hybrid approach has been used to isolate FIN219-interacting partners. A gene FIP2 (FIN219-interacting protein 2) encoding a glutamyl-tRNA synthetase (GluRS) was isolated. However, its function is not completely understood although previous studies showed that GluRS interacted with ABA INSENSITIVE 1 (ABI1) and Homeodomain protein 6 (AtHB6). By Co-IP Assays, we demonstrate a physical interaction between the full-length FIN219 and FIP2 in Nicotiana benthamiana, and this interaction occurred via the C terminus of FIN219 and the FIP2 in Arabidopsis thaliana. Besides, under FR and MeJA treatments, FIN219 and FIP2 can negatively regulate each other. Furthermore, the fip2 mutant shows insensitivity in hypocotyl growth to MeJA and ABA under FR light. Moreover, FIP2 overexpression results in the insensitivity of stomatal opening to MeJA and ABA. Interestingly, both FIP2 knocked-down mutant and overexpressor lines showed a defect in the number and the development of chloroplasts. Taken together, these data indicate that FIP2 may function via different mechanisms from shoot to root in ABA signaling and participate in the integration of FR light, JA and ABA signaling in Arabidopsis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:43:00Z (GMT). No. of bitstreams: 1 ntu-103-R00B42008-1.pdf: 17140544 bytes, checksum: e556cde3cfbfc8bd814b3e11fa1af5a4 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 目錄 I
圖與附圖目錄 IV 縮寫對照表 V 中文摘要 VI 英文摘要 VII 前言 1. 緒論 1 2. 植物的光訊息傳遞 2 2.1. 光敏素 2 2.2. 光訊息傳遞下游因子 3 3. 茉莉酸 3 3.1. 茉莉酸對植物生長發育的影響 4 3.2. 茉莉酸生合成途徑 4 3.3. 茉莉酸之訊息傳遞 5 3.4. 茉莉酸與光的調控機制 6 4. 離層酸 6 4.1. 離層酸對植物生長發育的影響 6 4.2. 離層酸生合成途徑 7 4.3. 離層酸與光調控機制 8 5. FIN219/JAR1 9 6. FIP2 10 7. 研究目標 10 材料與方法 1. 植物材料與生長條件 12 2. 下胚軸檢測 12 3. 根長檢測 12 4. 葉柄及葉型的檢測 13 5. 開花時間的檢測 13 6. 氣孔的檢測 13 7. 花青素檢測 14 8. 菸草系統中共免疫沈澱法 14 8.1. 質體之建構 14 8.2. 共免疫沈澱法(Coimmunoprecipitation Assay,Co-IP) 14 9. 基因表現分析 15 9.1. 總RNA萃取 15 9.2. 即時反轉錄聚合酶連鎖反應(Real-time RT-PCR) 15 10. 蛋白質表現分析 16 10.1. 總蛋白質的萃取 16 10.2. 西方墨點分析(Western blot) 16 11. 阿拉伯芥原生質體(PROTOPLAST)的分離 16 12. 切片製作 17 13. MeJA抑制百分比公試 18 結果 1. FIP2與FIN219蛋白質在植物體內的交互作用 19 2. 在不同光源下茉莉酸對於植物所產生的生理反應 20 3. 在遠紅光下,荷爾蒙對於植物所產生的生理反應 21 3.1. 照射不同光強度的遠紅光,並探討處理不同濃度的MeJA及ABA對阿拉伯芥下胚軸生長之影響 21 3.2. 不同光強度的遠紅光與MeJA、ABA對阿拉伯芥下胚軸之影響 22 3.3. 檢測弱遠紅光中MeJA對植物花青素累積含量之影響 23 3.4. 弱遠紅光下,處理MeJA對植物基因表現量之分析 24 3.5. 弱遠紅光與MeJA對植物蛋白質表現量之影響 25 4. 在白光中觀察FIP2突變體各種不同的生理反應 26 4.1. 原生質體以及葉綠體的形態 26 4.2. 葉片組織中葉綠體的結構和數量 27 4.3. 植物葉片生長發育的形態 27 4.4. FIP2影響阿拉伯芥的開花時間 28 5. 白光中生長的阿拉伯芥FIP2突變體與轉殖株對荷爾蒙的生理反應 30 5.1. 茉莉酸和離層酸的處理對fip2突變體與轉殖株氣孔開閉之影響 30 5.2. 茉莉酸和離層酸的處理對fip2突變體與轉殖株根部生長之影響 30 討論 1. FIP2與FIN219蛋白質在植物體內的交互作用 32 2. 在遠紅光中植物荷爾蒙對於FIP2及FIN219之影響 32 3. FIP2對於原生質體以及葉綠體的影響 34 4. FIP2對於植物葉片生長發育形態之影響 35 5. 在白光中植物荷爾蒙對FIP2及FIN219所產生的影響 35 6. 總結 36 參考文獻 37 結果圖片 46 附錄一、附圖 63 附錄二、實驗操作詳細流程與藥品配方 66 1. 阿拉伯芥RNA抽取(RNA藥品皆須滅菌40分鐘) 66 2. 植物總蛋白質萃取 67 3. 西方墨點分析 68 4. 阿拉伯芥原生質體分離 70 附錄三、引子表列 72 | |
dc.language.iso | zh-TW | |
dc.title | 阿拉伯芥中FIN219和FIN219-interacting Protein 2 (FIP2)的交互作用在茉莉酸、離層酸和光訊息傳遞中的功能性研究 | zh_TW |
dc.title | Functional Studies of FIN219 and FIN219-interacting Protein 2 (FIP2) Interaction in the Integration of Jasmonate, Abscisic Acid and Light Signaling in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳素幸(Shu-Hsing Wu,),鄭秋萍(Chiu-Ping Cheng),張英?(Ing-Feng Chang),張孟基(Men-Chi Chang) | |
dc.subject.keyword | 光,離層酸,茉莉酸, | zh_TW |
dc.subject.keyword | Jasmonate,Abscisic Acid,Light, | en |
dc.relation.page | 72 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-12 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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