含光敏感分子之奈米電紡絲纖維應用於癌症之熱治療

Abstract

溫熱療法，將癌細胞組織加熱到 41~45 oC 可以明顯增加使用放射或是化學 性治療法的效果。本論文目的是為了發展出含有光敏感分子之電紡絲應用於溫熱 至療法來治療表面癌症腫瘤，例如皮膚癌。聚左乳酸與銅葉綠素鈉所噴成的電紡 絲，可以經由照射 532 nm 的綠光雷射來加熱，達到殺死細胞的目的。 在第二部分我們將苯乙烯與銅葉綠素鈉聚合以後，得到接枝有銅葉綠素鈉單 體的聚苯乙烯，並且將此產物噴成電紡絲後來應用，之後經由 MTT assay、染 trypan blue、live/dead 三種實驗來觀察綠光雷射強度與照射時間對細胞的影響，最後達到可以控制照射綠光雷射的強度與照射時間，來達到殺死癌細胞的目的，並且不會對周遭正常細胞造成明顯損害，我們可以將此結果應用於溫熱治療法來治療大範圍表面癌症腫瘤。

Hyperthermia, the heating of cancerous tissues to between 41 and 45 oC, has been shown to improve the efficacy of cancer therapy when used in conjunction with irradiation and/or chemotherapy. The objective of this study was to develop an incorporation of electrospun sheet with photosensitizer for photodynamic therapy in surface cancer tumor, such as skin cancer. The blend of poly-L-lactic acid (PLLA) and chlorophyllin sodium copper (CSC) was electrospinning a sheet was developed. The electrospun sheets could be remotely heated upon exposure to an external wave length 532 nm green laser to kill cells. In the second part of the study, for the purpose to produce high percentage chlorophyllin sodium copper contain polystyrene , we used chlorophyllin sodium copper and styrene with ATRP, and electrospinning to sheet for the hyperthermia purpose. Then we used MTT assay、trypan blue stain and live/dead stain to observe the effect of green laser intensity and irradiation time to the cell viability. Finally we can achieve the purpose of killing cancer cell by control the green laser intensity and irradiation time without hurting the normal cell around. We can use this result on the hyperthermia of in surface cancer tumor, such as skin cancer.